An orthotopic model of anaplastic thyroid carcinoma in athymic nude mice

Seungwon Kim, Young Wook Park, Bradley A. Schiff, Dao D. Doan, Yasemin Yazici, Samar A. Jasser, Maher Younes, Mahitosh Mandal, Benjamin N. Bekele, Jeffrey N. Myers

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Purpose: To develop an orthotopic model of anaplastic thyroid carcinoma (ATC) in athymic nude mice. Experimental Design: Various thyroid carcinoma cell lines were injected into the thyroid gland of athymic nude mice to determine whether such injection was technically feasible. ATC cells were then injected into the thyroid gland or the subcutis of nude mice at various concentrations, and the mice were then followed for tumor development. The tumors were examined histopathologically for local invasion or regional or distant metastasis. Results: Injection of tumor cells into the thyroid glands of nude mice was technically feasible and resulted in the formation of thyroid tumors. The ATC cell line DRO showed significantly higher tumorigenicity in the thyroid gland than in the subcutis. In contrast, oral squamous cell carcinoma cell line TU167 shows no significantly higher tumorigenicity in the thyroid gland than in the subcutis. ATC tumors established in the thyroid gland also produced symptomatic compression of the esophagus and the trachea. Local invasion of the larynx and trachea was as well as high rates of pulmonary metastasis were also observed. Immunohistochemical staining showed higher microvessel density as well as higher expression of vascular endothelial growth factor and interleukin-8 in the orthotopic thyroid tumors than in ectopic tumors. Conclusion: An orthotopic model of ATC in athymic nude mice was developed that closely recapitulates the clinical findings of human ATC. This model should facilitate the understanding of the pathogenesis of ATC and aid in the development of novel therapies against ATC.

Original languageEnglish (US)
Pages (from-to)1713-1721
Number of pages9
JournalClinical Cancer Research
Volume11
Issue number5
DOIs
StatePublished - Mar 1 2005
Externally publishedYes

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Nude Mice
Thyroid Gland
Neoplasms
Trachea
Cell Line
Neoplasm Metastasis
Injections
Anaplastic Thyroid Carcinoma
Larynx
Microvessels
Interleukin-8
Thyroid Neoplasms
Vascular Endothelial Growth Factor A
Esophagus
Squamous Cell Carcinoma
Research Design
Staining and Labeling
Lung

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Kim, S., Park, Y. W., Schiff, B. A., Doan, D. D., Yazici, Y., Jasser, S. A., ... Myers, J. N. (2005). An orthotopic model of anaplastic thyroid carcinoma in athymic nude mice. Clinical Cancer Research, 11(5), 1713-1721. https://doi.org/10.1158/1078-0432.CCR-04-1908

An orthotopic model of anaplastic thyroid carcinoma in athymic nude mice. / Kim, Seungwon; Park, Young Wook; Schiff, Bradley A.; Doan, Dao D.; Yazici, Yasemin; Jasser, Samar A.; Younes, Maher; Mandal, Mahitosh; Bekele, Benjamin N.; Myers, Jeffrey N.

In: Clinical Cancer Research, Vol. 11, No. 5, 01.03.2005, p. 1713-1721.

Research output: Contribution to journalArticle

Kim, S, Park, YW, Schiff, BA, Doan, DD, Yazici, Y, Jasser, SA, Younes, M, Mandal, M, Bekele, BN & Myers, JN 2005, 'An orthotopic model of anaplastic thyroid carcinoma in athymic nude mice', Clinical Cancer Research, vol. 11, no. 5, pp. 1713-1721. https://doi.org/10.1158/1078-0432.CCR-04-1908
Kim, Seungwon ; Park, Young Wook ; Schiff, Bradley A. ; Doan, Dao D. ; Yazici, Yasemin ; Jasser, Samar A. ; Younes, Maher ; Mandal, Mahitosh ; Bekele, Benjamin N. ; Myers, Jeffrey N. / An orthotopic model of anaplastic thyroid carcinoma in athymic nude mice. In: Clinical Cancer Research. 2005 ; Vol. 11, No. 5. pp. 1713-1721.
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