An organizing region in metamorphosing hydrozoan planula larvae - Stimulation of axis formation in both larval and in adult tissue

Melanie Stumpf, Britta Will, Karola Wittig, Jennifer Kasper, Benjamin Fischer, Jürgen Schmich, Stefanie Seipp, Thomas Leitz

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A novel wingless gene was isolated from the marine colonial hydroid Hydractinia echinata. Alignments and Bayesian inference analysis clearly assign the gene to the Wnt5A group. In line with data found for the brachyury ortholog of Hydractinia, He-wnt5A is expressed during metamorphosis in the posterior tip of the spindle-shaped planula larva, suggesting that the tip functions as a putative organizer during metamorphosis. Additionally, the outermost cells of the posterior tip are omitted from apoptosis during metamorphosis. In order to investigate this putative organizer function, we transplanted the posterior tip of metamorphosing animals into non-induced larvae and into primary polyps 24 h and 48 h of age. In larvae, the tip induced formation of a secondary axis. In polyps the building of ectopic head structures was induced. Based on our data on axis formation, on gene expression similar to the organizers of other species, and the absence of regular apoptosis, we conclude that the posterior tip of the Hydractinia larva has organizing activity during metamorphosis.

Original languageEnglish (US)
Pages (from-to)795-802
Number of pages8
JournalInternational Journal of Developmental Biology
Volume54
Issue number5
DOIs
StatePublished - 2010
Externally publishedYes

Fingerprint

Larva
Polyps
Apoptosis
Bayes Theorem
Genes
Head
Gene Expression

Keywords

  • Brachyury
  • Hydractinia echinata
  • Organizer
  • Planula larva
  • Wnt

ASJC Scopus subject areas

  • Developmental Biology
  • Embryology
  • Medicine(all)

Cite this

An organizing region in metamorphosing hydrozoan planula larvae - Stimulation of axis formation in both larval and in adult tissue. / Stumpf, Melanie; Will, Britta; Wittig, Karola; Kasper, Jennifer; Fischer, Benjamin; Schmich, Jürgen; Seipp, Stefanie; Leitz, Thomas.

In: International Journal of Developmental Biology, Vol. 54, No. 5, 2010, p. 795-802.

Research output: Contribution to journalArticle

Stumpf, Melanie ; Will, Britta ; Wittig, Karola ; Kasper, Jennifer ; Fischer, Benjamin ; Schmich, Jürgen ; Seipp, Stefanie ; Leitz, Thomas. / An organizing region in metamorphosing hydrozoan planula larvae - Stimulation of axis formation in both larval and in adult tissue. In: International Journal of Developmental Biology. 2010 ; Vol. 54, No. 5. pp. 795-802.
@article{1049780e4b3548758bb354784e613212,
title = "An organizing region in metamorphosing hydrozoan planula larvae - Stimulation of axis formation in both larval and in adult tissue",
abstract = "A novel wingless gene was isolated from the marine colonial hydroid Hydractinia echinata. Alignments and Bayesian inference analysis clearly assign the gene to the Wnt5A group. In line with data found for the brachyury ortholog of Hydractinia, He-wnt5A is expressed during metamorphosis in the posterior tip of the spindle-shaped planula larva, suggesting that the tip functions as a putative organizer during metamorphosis. Additionally, the outermost cells of the posterior tip are omitted from apoptosis during metamorphosis. In order to investigate this putative organizer function, we transplanted the posterior tip of metamorphosing animals into non-induced larvae and into primary polyps 24 h and 48 h of age. In larvae, the tip induced formation of a secondary axis. In polyps the building of ectopic head structures was induced. Based on our data on axis formation, on gene expression similar to the organizers of other species, and the absence of regular apoptosis, we conclude that the posterior tip of the Hydractinia larva has organizing activity during metamorphosis.",
keywords = "Brachyury, Hydractinia echinata, Organizer, Planula larva, Wnt",
author = "Melanie Stumpf and Britta Will and Karola Wittig and Jennifer Kasper and Benjamin Fischer and J{\"u}rgen Schmich and Stefanie Seipp and Thomas Leitz",
year = "2010",
doi = "10.1387/ijdb.082738ms",
language = "English (US)",
volume = "54",
pages = "795--802",
journal = "International Journal of Developmental Biology",
issn = "0214-6282",
publisher = "University of the Basque Country Press",
number = "5",

}

TY - JOUR

T1 - An organizing region in metamorphosing hydrozoan planula larvae - Stimulation of axis formation in both larval and in adult tissue

AU - Stumpf, Melanie

AU - Will, Britta

AU - Wittig, Karola

AU - Kasper, Jennifer

AU - Fischer, Benjamin

AU - Schmich, Jürgen

AU - Seipp, Stefanie

AU - Leitz, Thomas

PY - 2010

Y1 - 2010

N2 - A novel wingless gene was isolated from the marine colonial hydroid Hydractinia echinata. Alignments and Bayesian inference analysis clearly assign the gene to the Wnt5A group. In line with data found for the brachyury ortholog of Hydractinia, He-wnt5A is expressed during metamorphosis in the posterior tip of the spindle-shaped planula larva, suggesting that the tip functions as a putative organizer during metamorphosis. Additionally, the outermost cells of the posterior tip are omitted from apoptosis during metamorphosis. In order to investigate this putative organizer function, we transplanted the posterior tip of metamorphosing animals into non-induced larvae and into primary polyps 24 h and 48 h of age. In larvae, the tip induced formation of a secondary axis. In polyps the building of ectopic head structures was induced. Based on our data on axis formation, on gene expression similar to the organizers of other species, and the absence of regular apoptosis, we conclude that the posterior tip of the Hydractinia larva has organizing activity during metamorphosis.

AB - A novel wingless gene was isolated from the marine colonial hydroid Hydractinia echinata. Alignments and Bayesian inference analysis clearly assign the gene to the Wnt5A group. In line with data found for the brachyury ortholog of Hydractinia, He-wnt5A is expressed during metamorphosis in the posterior tip of the spindle-shaped planula larva, suggesting that the tip functions as a putative organizer during metamorphosis. Additionally, the outermost cells of the posterior tip are omitted from apoptosis during metamorphosis. In order to investigate this putative organizer function, we transplanted the posterior tip of metamorphosing animals into non-induced larvae and into primary polyps 24 h and 48 h of age. In larvae, the tip induced formation of a secondary axis. In polyps the building of ectopic head structures was induced. Based on our data on axis formation, on gene expression similar to the organizers of other species, and the absence of regular apoptosis, we conclude that the posterior tip of the Hydractinia larva has organizing activity during metamorphosis.

KW - Brachyury

KW - Hydractinia echinata

KW - Organizer

KW - Planula larva

KW - Wnt

UR - http://www.scopus.com/inward/record.url?scp=77956045885&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956045885&partnerID=8YFLogxK

U2 - 10.1387/ijdb.082738ms

DO - 10.1387/ijdb.082738ms

M3 - Article

C2 - 20336614

AN - SCOPUS:77956045885

VL - 54

SP - 795

EP - 802

JO - International Journal of Developmental Biology

JF - International Journal of Developmental Biology

SN - 0214-6282

IS - 5

ER -