An Msh2 Point Mutation Uncouples DNA Mismatch Repair and Apoptosis

Diana P. Lin, Yuxun Wang, Stefan J. Scherer, Alan B. Clark, Kan Yang, Elena Avdievich, Bo Jin, Uwe Werling, Tchaiko Parris, Naoto Kurihara, Asad Umar, Raju Kucherlapati, Martin Lipkin, Thomas A. Kunkel, Winfried Edelmann

Research output: Contribution to journalArticle

140 Scopus citations

Abstract

Mutations in the human DNA mismatch repair gene MSH2 are associated with hereditary nonpolyposis colorectal cancer as well as a significant proportion of sporadic colorectal cancer. The inactivation of MSH2 results in the accumulation of somatic mutations in the genome of tumor cells and resistance to the genotoxic effects of a variety of chemotherapeutic agents. Here we show that the DNA repair and DNA damage-induced apoptosis functions of Msh2 can be uncoupled using mice that carry the G674A missense mutation in the conserved ATPase domain. As a consequence, although Msh2G674A homozygous mutant mice are highly tumor prone, the onset of tumorigenesis is delayed as compared with Msh2-null mice. In addition, tumors that carry the mutant allele remain responsive to treatment with a chemotherapeutic agent. Our results indicate that Msh2-mediated apoptosis is an important component of tumor suppression and that certain MSH2 missense mutations can cause mismatch repair deficiency while retaining the signaling functions that confer sensitivity to chemotherapeutic agents.

Original languageEnglish (US)
Pages (from-to)517-522
Number of pages6
JournalCancer Research
Volume64
Issue number2
DOIs
StatePublished - Jan 15 2004

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Lin, D. P., Wang, Y., Scherer, S. J., Clark, A. B., Yang, K., Avdievich, E., Jin, B., Werling, U., Parris, T., Kurihara, N., Umar, A., Kucherlapati, R., Lipkin, M., Kunkel, T. A., & Edelmann, W. (2004). An Msh2 Point Mutation Uncouples DNA Mismatch Repair and Apoptosis. Cancer Research, 64(2), 517-522. https://doi.org/10.1158/0008-5472.CAN-03-2957