An in vitro method for comparing biocompatibility of materials for extracorporeal circulation

Giles J. Peek, Richard Scott, Hilliary M. Killer, Richard K. Firmin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We measured the response of fresh heparinized human blood to recirculation through circuits made of LVA (Portex Industries, Hythe, Kent, UK), SRT (Rehau UK, Langley, Slough, UK) and Tygon® S-65-HL (Norton Performance Plastics, Corby, Northants, UK), as control. Circuit construction: 1/2 in. tubing, heat exchanger (Dideco D-720P), Stockert roller pump, just underoccluded, Cincinnati Sub Zero heater, circuit volume of 500 ml. Flow 3.45 l/min, 37°C. Samples: at 10 min, 1, 2, 4 and 6 h. n=5 in each group; 2/5 SRT experiments were stopped at 45 and 60 min due to overpressurization. Results: Baseline activated clotting time (ACT) of 300 s, increasing in all groups as fibrinogen fell to zero with SRT and LVA. Minimum fibrinogen was 1 g/l for Tygon. Absolute thrombocytopenia occurred (SRT and LVA 60 min and Tygon 240 min). International normalized ratio (INR) in both the SRT and LVA circuits increased, but mean increase for Tygon (0.56) was smaller than the other two materials. Plasma free haemoglobin increased in all three materials; the increase was greater in the LVA circuits compared to the control. C5b9 levels increased equally in all groups. Lactoferrin levels rose equally in all groups to a maximum at 150 min. The neutrophil counts fell, mirroring the lactoferrin. The total white cell counts also fell in all groups; in the LVA circuits, the fall was significantly lower than in the control. Rapid disappearance of platelets and fibrinogen from the blood in the SRT and LVA circuits excludes them both from extracorporeal use. Paradoxically, SRT caused the least complement activation of the three materials. This method can be used to compare biocompatibility.

Original languageEnglish (US)
Pages (from-to)125-132
Number of pages8
JournalPerfusion
Volume17
Issue number2
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Extracorporeal Circulation
Biocompatibility
Fibrinogen
Lactoferrin
Networks (circuits)
International Normalized Ratio
Group
Complement Activation
Thrombocytopenia
Plastics
Industry
Hemoglobins
Neutrophils
Blood Platelets
Cell Count
Hot Temperature
Blood
activation
heat
Hemoglobin

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

An in vitro method for comparing biocompatibility of materials for extracorporeal circulation. / Peek, Giles J.; Scott, Richard; Killer, Hilliary M.; Firmin, Richard K.

In: Perfusion, Vol. 17, No. 2, 2002, p. 125-132.

Research output: Contribution to journalArticle

Peek, Giles J. ; Scott, Richard ; Killer, Hilliary M. ; Firmin, Richard K. / An in vitro method for comparing biocompatibility of materials for extracorporeal circulation. In: Perfusion. 2002 ; Vol. 17, No. 2. pp. 125-132.
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