An in vitro autogene

Eric A. Davidson; Adam J. Meyer; Jared W. Ellefson; Matthew Levy; Andrew D. Ellington

doi:10.1021/sb3000113

An in vitro autogene

Eric A. Davidson, Adam J. Meyer, Jared W. Ellefson, Matthew Levy, Andrew D. Ellington

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Recent technological advances have allowed development of increasingly complex systems for in vitro evolution. Here, we describe an in vitro autogene composed of a self-amplifying T7 RNA polymerase system. Functional autogene templates in cell-free lysate produce T7 RNA polymerase, which amplifies the autogene genetic information through a positive feedback architecture. Compartmentalization of individual templates within a water-inoil emulsion links genotype and phenotype, allowing evolution.

Original language	English (US)
Pages (from-to)	190-196
Number of pages	7
Journal	ACS Synthetic Biology
Volume	1
Issue number	5
DOIs	https://doi.org/10.1021/sb3000113
State	Published - May 18 2012
Externally published	Yes

Keywords

Autogene
In vitro compartmentalization
In vitro selection
Self-amplifying genetic system
T7 RNA polymerase

ASJC Scopus subject areas

Biomedical Engineering
Biochemistry, Genetics and Molecular Biology (miscellaneous)

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10.1021/sb3000113

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AU - Davidson, Eric A.

AU - Meyer, Adam J.

AU - Ellefson, Jared W.

AU - Levy, Matthew

AU - Ellington, Andrew D.

PY - 2012/5/18

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AB - Recent technological advances have allowed development of increasingly complex systems for in vitro evolution. Here, we describe an in vitro autogene composed of a self-amplifying T7 RNA polymerase system. Functional autogene templates in cell-free lysate produce T7 RNA polymerase, which amplifies the autogene genetic information through a positive feedback architecture. Compartmentalization of individual templates within a water-inoil emulsion links genotype and phenotype, allowing evolution.

KW - Autogene

KW - In vitro compartmentalization

KW - In vitro selection

KW - Self-amplifying genetic system

KW - T7 RNA polymerase

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An in vitro autogene

Abstract

Keywords

ASJC Scopus subject areas

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