An in vitro autogene

Eric A. Davidson, Adam J. Meyer, Jared W. Ellefson, Matthew Levy, Andrew D. Ellington

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Recent technological advances have allowed development of increasingly complex systems for in vitro evolution. Here, we describe an in vitro autogene composed of a self-amplifying T7 RNA polymerase system. Functional autogene templates in cell-free lysate produce T7 RNA polymerase, which amplifies the autogene genetic information through a positive feedback architecture. Compartmentalization of individual templates within a water-inoil emulsion links genotype and phenotype, allowing evolution.

Original languageEnglish (US)
Pages (from-to)190-196
Number of pages7
JournalACS Synthetic Biology
Volume1
Issue number5
DOIs
StatePublished - May 18 2012

Keywords

  • Autogene
  • In vitro compartmentalization
  • In vitro selection
  • Self-amplifying genetic system
  • T7 RNA polymerase

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

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