An in vitro autogene

Eric A. Davidson, Adam J. Meyer, Jared W. Ellefson, Matthew Levy, Andrew D. Ellington

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Recent technological advances have allowed development of increasingly complex systems for in vitro evolution. Here, we describe an in vitro autogene composed of a self-amplifying T7 RNA polymerase system. Functional autogene templates in cell-free lysate produce T7 RNA polymerase, which amplifies the autogene genetic information through a positive feedback architecture. Compartmentalization of individual templates within a water-inoil emulsion links genotype and phenotype, allowing evolution.

Original languageEnglish (US)
Pages (from-to)190-196
Number of pages7
JournalACS Synthetic Biology
Volume1
Issue number5
DOIs
Publication statusPublished - May 18 2012

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Keywords

  • Autogene
  • In vitro compartmentalization
  • In vitro selection
  • Self-amplifying genetic system
  • T7 RNA polymerase

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Davidson, E. A., Meyer, A. J., Ellefson, J. W., Levy, M., & Ellington, A. D. (2012). An in vitro autogene. ACS Synthetic Biology, 1(5), 190-196. https://doi.org/10.1021/sb3000113