An improved method for generating BAC DNA suitable for FISH

J. Roohi, M. Cammer, Cristina Montagna, E. Hatchwell

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Fluorescence in situ hybridization (FISH) is commonly used to identify chromosomal aberrations such as translocations, deletions, duplications, gene fusions, and aneuploidies. It relies on the hybridization of fluorescently labeled DNA probes onto denatured metaphase chromosomes or interphase nuclei. These probes are often generated from DNA sequences cloned within bacterial artificial chromosomes (BACs). Growing these BACs in adequate amounts for FISH can be demanding. We describe FISH performed with bacteriophage Phi29 DNA polymerase amplified BAC DNA. Generating this material required significantly smaller cultures and less time than standard methods. The FISH results obtained were comparable with those obtained from standard BAC DNA. We believe this method of BAC DNA generation is useful for the entire FISH community as it improves considerably on prior methods.

Original languageEnglish (US)
Pages (from-to)7-9
Number of pages3
JournalCytogenetic and Genome Research
Volume121
Issue number1
DOIs
StatePublished - Jun 2008

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Bacterial Artificial Chromosomes
Fluorescence In Situ Hybridization
DNA
Gene Fusion
Interphase
DNA Probes
Aneuploidy
DNA-Directed DNA Polymerase
Metaphase
Chromosome Aberrations
Bacteriophages
Chromosomes

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

An improved method for generating BAC DNA suitable for FISH. / Roohi, J.; Cammer, M.; Montagna, Cristina; Hatchwell, E.

In: Cytogenetic and Genome Research, Vol. 121, No. 1, 06.2008, p. 7-9.

Research output: Contribution to journalArticle

Roohi, J. ; Cammer, M. ; Montagna, Cristina ; Hatchwell, E. / An improved method for generating BAC DNA suitable for FISH. In: Cytogenetic and Genome Research. 2008 ; Vol. 121, No. 1. pp. 7-9.
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