An immunohistochemical method for detecting bradyzoite antigen (BAG5) in toxoplasma gondii-infected tissues cross-reacts with a Neospora caninum bradyzoite antigen

Milton M. McAllister, Stephen F. Parmley, Louis M. Weiss, Valerie J. Welch, Angela M. McGuire

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

The previously cloned gene of a bradyzoite-specific antigen (BAG5) of Toxoplasma gondii was used to express a fusion protein for subsequent antiserum production in rabbits. The BAG5 antiserum was used in an immunohistochemical procedure to look for reactive epitopes in bradyzoites and tachyzoites of T. gondii within animal tissues. Encysted bradyzoites in brain were stained deeply and diffusely. Although most unencysted organisms in brain were not stained, occasional free organisms had mild to deep staining. There was no staining of tachyzoites in liver where cysts were not observed. Neospora caninum organisms within animal tissues were also examined using the BAG5 immunohistochemical procedure. The BAG5 antiserum cross- reacted with N. caninum bradyzoites but had no affinity for tachyzoites.

Original languageEnglish (US)
Pages (from-to)354-355
Number of pages2
JournalJournal of Parasitology
Volume82
Issue number2
DOIs
StatePublished - Apr 1996

Fingerprint

bradyzoites
Neospora
tachyzoites
Neospora caninum
Toxoplasma
Toxoplasma gondii
antigen
antiserum
Immune Sera
animal tissues
antigens
Antigens
brain
organisms
Staining and Labeling
animal
Brain
cyst
epitopes
Cysts

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

An immunohistochemical method for detecting bradyzoite antigen (BAG5) in toxoplasma gondii-infected tissues cross-reacts with a Neospora caninum bradyzoite antigen. / McAllister, Milton M.; Parmley, Stephen F.; Weiss, Louis M.; Welch, Valerie J.; McGuire, Angela M.

In: Journal of Parasitology, Vol. 82, No. 2, 04.1996, p. 354-355.

Research output: Contribution to journalArticle

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