An immunochemical approach to the identification of the MBTA binding site of the nicotinic acetylcholine receptor of Torpedo californica

Sean M. Cahill, Jakob Schmidt

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Monospecific anti-[4-(N-maleimidobenzyl)trimethylammonium] (MBTA) antibodies were prepared from sera of rabbits immunized with an albumin-MBTA conjugate and used to synthesize an MBTA-specific immunosorbent. Torpedo californica acetylcholine receptor was affinity labeled with [3H]-MBTA and proteolyzed extensively with pronase, and the peptide fraction of the digest chromatographed on the anti-MBTA resin. The amino acid composition of the purified MBTA-peptide fraction was compared with the sequences flanking the seven cysteinyl residues of the α-subunit. The best fit was observed with the segment containing cysteine 142.

Original languageEnglish (US)
Pages (from-to)602-608
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume122
Issue number2
DOIs
StatePublished - Jul 31 1984
Externally publishedYes

Fingerprint

Torpedo
Nicotinic Receptors
Binding Sites
Immunosorbents
Pronase
Peptides
Cholinergic Receptors
Cysteine
Albumins
Resins
Rabbits
Amino Acids
Antibodies
Serum
Chemical analysis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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