An immunochemical approach to the identification of the MBTA binding site of the nicotinic acetylcholine receptor of Torpedo californica

Sean Cahill, Jakob Schmidt

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Monospecific anti-[4-(N-maleimidobenzyl)trimethylammonium] (MBTA) antibodies were prepared from sera of rabbits immunized with an albumin-MBTA conjugate and used to synthesize an MBTA-specific immunosorbent. Torpedo californica acetylcholine receptor was affinity labeled with [3H]-MBTA and proteolyzed extensively with pronase, and the peptide fraction of the digest chromatographed on the anti-MBTA resin. The amino acid composition of the purified MBTA-peptide fraction was compared with the sequences flanking the seven cysteinyl residues of the α-subunit. The best fit was observed with the segment containing cysteine 142.

Original languageEnglish (US)
Pages (from-to)602-608
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume122
Issue number2
DOIs
StatePublished - Jul 31 1984
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'An immunochemical approach to the identification of the MBTA binding site of the nicotinic acetylcholine receptor of Torpedo californica'. Together they form a unique fingerprint.

Cite this