An HDAC3-PROX1 corepressor module acts on HNF4α to control hepatic triglycerides

Sean M. Armour, Jarrett R. Remsberg, Manashree Damle, Simone Sidoli, Wesley Y. Ho, Zhenghui Li, Benjamin A. Garcia, Mitchell A. Lazar

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The histone deacetylase HDAC3 is a critical mediator of hepatic lipid metabolism, and liver-specific deletion of HDAC3 leads to fatty liver. To elucidate the underlying mechanism, here we report a method of cross-linking followed by mass spectrometry to define a high-confidence HDAC3 interactome in vivo that includes the canonical NCoR-HDAC3 complex as well as Prospero-related homeobox 1 protein (PROX1). HDAC3 and PROX1 co-localize extensively on the mouse liver genome, and are co-recruited by hepatocyte nuclear factor 4α (HNF4α). The HDAC3-PROX1 module controls the expression of a gene program regulating lipid homeostasis, and hepatic-specific ablation of either component increases triglyceride content in liver. These findings underscore the importance of specific combinations of transcription factors and coregulators in the fine tuning of organismal metabolism.

Original languageEnglish (US)
Article number54
JournalNature communications
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2017

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ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Armour, S. M., Remsberg, J. R., Damle, M., Sidoli, S., Ho, W. Y., Li, Z., Garcia, B. A., & Lazar, M. A. (2017). An HDAC3-PROX1 corepressor module acts on HNF4α to control hepatic triglycerides. Nature communications, 8(1), [54]. https://doi.org/10.1038/s41467-017-00772-5