TY - JOUR
T1 - An enhancer at the 3′ end of the mouse immunoglobulin heavy chain locus
AU - Lieberson, Rebecca
AU - Giannini, Sandra L.
AU - Birshtein, Barbara K.
AU - Eckhardt, Laurel A.
N1 - Funding Information:
The authors would like to thank Hanna Radomska for expert technical assistance and Maryellen Kurkulos for technical advice. We also thank Michael Anderson and Sherie Morrison for CAT vectors and helpful advice on transient transfections of J558L. This work was supported by grants from the National Institutes of Health (AI3O653 to L.A.E and AI13509, AI10702, and PC 3OCA13333O to B.K.B.). Additional support came from the Irvington House Institute for Medical Research (B.K.B.) and from an N.I.H. predoctoral training grant (CA09173 to S.L.G.).
PY - 1991/2/25
Y1 - 1991/2/25
N2 - A tissue-specific enhancer (Eμ) lies between the joining (JH) and μ constant region (Cμ) gene segments of the immunogiobulin heavy chain (igH) locus. Since mouse endogenous igH genes are efficiently transcribed in its absence, the normal function of this enhancer remains ill-defined. Recently, another lymphold-specific enhancer of equal strength has been identified 3′ of the rat IgH locus. We have isolated an analogous sequence from mouse and have mapped it 12.5kb 3′ of the 3′-most constant region gene (Cα-membrane) of the BALB/c mouse locus. The mouse and rat sequences are 82% homologous and share with other enhancers several DNA sequence motifs capable of binding protein. However, In transient transfection assays, the mouse sequence behaves as a weaker enhancer. The role of this distant element in the expression of endogenous IgH genes, both In Eμ-deficient, ig producing cell lines and during normal B cell development, is discussed.
AB - A tissue-specific enhancer (Eμ) lies between the joining (JH) and μ constant region (Cμ) gene segments of the immunogiobulin heavy chain (igH) locus. Since mouse endogenous igH genes are efficiently transcribed in its absence, the normal function of this enhancer remains ill-defined. Recently, another lymphold-specific enhancer of equal strength has been identified 3′ of the rat IgH locus. We have isolated an analogous sequence from mouse and have mapped it 12.5kb 3′ of the 3′-most constant region gene (Cα-membrane) of the BALB/c mouse locus. The mouse and rat sequences are 82% homologous and share with other enhancers several DNA sequence motifs capable of binding protein. However, In transient transfection assays, the mouse sequence behaves as a weaker enhancer. The role of this distant element in the expression of endogenous IgH genes, both In Eμ-deficient, ig producing cell lines and during normal B cell development, is discussed.
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U2 - 10.1093/nar/19.4.933
DO - 10.1093/nar/19.4.933
M3 - Article
C2 - 1901991
AN - SCOPUS:0025969990
SN - 0305-1048
VL - 19
SP - 933
EP - 937
JO - Nucleic acids research
JF - Nucleic acids research
IS - 4
ER -