An endoplasmic reticulum-retained herpes simplex virus glycoprotein H is absent from secreted virions: Evidence for reenvelopment during egress

Helena Browne, Susanne Bell, Tony Minson, Duncan W. Wilson

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

Although it is generally accepted that one of the first steps of herpesvirus egress is the acquisition of an envelope by nucleocapsids budding into the inner nuclear membrane, later events in the pathway are not well understood. We tested the hypothesis that the virus then undergoes de- envelopment, followed by reenvelopment at membranes outside the endoplasmic reticulum (ER), by constructing a recombinant virus in which the expression of an essential glycoprotein, gH, is restricted to the inner nuclear membrane-ER by means of the ER retention motif, KKXX. This targeting signal conferred the predicted ER localization properties on gH in recombinant virus-infected cells, and gH and gL polypeptides failed to become processed to their mature forms. Cells infected with the recombinant virus released particles with 100-fold less infectivity than those released by cells infected with the wild-type parent virus, yet the number of enveloped virus particles released into the medium was unaltered. These particles contained normal amounts of gD and VP16 but did not contain detectable amounts of gH, and these data are consistent with a model of virus exit whereby naked nucleocapsids in the cytoplasm acquire their final envelope from a subcellular compartment other than the ER-inner nuclear membrane.

Original languageEnglish (US)
Pages (from-to)4311-4316
Number of pages6
JournalJournal of Virology
Volume70
Issue number7
StatePublished - 1996

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herpes simplex
Simplexvirus
virion
Endoplasmic Reticulum
Virion
endoplasmic reticulum
glycoproteins
Glycoproteins
Viruses
Nuclear Envelope
nuclear membrane
viruses
Nucleocapsid
nucleocapsid
Herpesviridae
cells
polypeptides
Cytoplasm
pathogenicity
cytoplasm

ASJC Scopus subject areas

  • Immunology

Cite this

An endoplasmic reticulum-retained herpes simplex virus glycoprotein H is absent from secreted virions : Evidence for reenvelopment during egress. / Browne, Helena; Bell, Susanne; Minson, Tony; Wilson, Duncan W.

In: Journal of Virology, Vol. 70, No. 7, 1996, p. 4311-4316.

Research output: Contribution to journalArticle

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