An embryonic enhancer determines the temporal activation of a sea urchin late H1 gene.

Z. C. Lai, D. J. DeAngelo, M. DiLiberto, Geoffrey J. Childs

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Normal development requires that individual genes be expressed in their correct temporal patterns, but the mechanisms regulating this process during early embryogenesis are poorly understood. We have studied the early and late sea urchin histone genes during embryogenesis to address the molecular mechanisms controlling temporal gene expression. By measuring the changes in expression of cloned H1-beta DNA constructs after microinjection into fertilized one-cell zygotes, we demonstrated that a highly conserved 30-base-pair segment of DNA between positions -288 and -317 (USE IV) is responsible for the transcriptional activation of this late histone gene at the late blastula stage. In this report, we demonstrate that an oligonucleotide corresponding to USE IV acts as an embryonic enhancer element capable of activating the simian virus 40 early promoter in a stage-specific manner. Using an in vivo competition assay and in vitro DNase I footprinting and mobility shift assays, we also identified a protein(s) that interacts with this enhancer. Results of the competition assay suggested that this factor acts to stimulate transcription of the H1-beta gene. The factor was found to be stored in mature eggs as well as in all embryonic stages examined. The mobility of the factor found in eggs, however, differed from that of the embryonic form, which suggested that posttranslational modification occurs after fertilization.

Original languageEnglish (US)
Pages (from-to)2315-2321
Number of pages7
JournalMolecular and Cellular Biology
Volume9
Issue number6
StatePublished - Jun 1989

Fingerprint

Sea Urchins
Histones
Eggs
Genes
Embryonic Development
Blastula
Simian virus 40
Zygote
Deoxyribonuclease I
DNA
Microinjections
Electrophoretic Mobility Shift Assay
Post Translational Protein Processing
Fertilization
Oligonucleotides
Base Pairing
Transcriptional Activation
Gene Expression
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

An embryonic enhancer determines the temporal activation of a sea urchin late H1 gene. / Lai, Z. C.; DeAngelo, D. J.; DiLiberto, M.; Childs, Geoffrey J.

In: Molecular and Cellular Biology, Vol. 9, No. 6, 06.1989, p. 2315-2321.

Research output: Contribution to journalArticle

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