TY - JOUR
T1 - An efficient rice transformation system utilizing mature seed-derived explants and a portable, inexpensive particle bombardment device
AU - Sudhakar, Durailagaraja
AU - Duc, Le Tan
AU - Bong, Bui Ba
AU - Tinjuangjun, Porntip
AU - Maqbool, Shahina Bano
AU - Valdez, Marta
AU - Jefferson, Richard
AU - Christou, Paul
PY - 1998/1/1
Y1 - 1998/1/1
N2 - We developed a practical and efficient gene transfer system for indica rice utilizing mature-seed derived explants and a simple bombardment device which uses compressed helium for accelerating DNA-coated metal particles. Unlike instruments which have been described in the literature previously, this new bombardment device, which is an improvement of the particle inflow concept, does not require vacuum. This attribute simplifies the transformation procedure significantly and it makes rice transformation technology accessible to laboratories which may not have the resources to invest in more expensive particle bombardment instruments. We determined experimentally that we could recover transgenic rice plants utilizing three different particle bombardment instruments at comparable frequencies.
AB - We developed a practical and efficient gene transfer system for indica rice utilizing mature-seed derived explants and a simple bombardment device which uses compressed helium for accelerating DNA-coated metal particles. Unlike instruments which have been described in the literature previously, this new bombardment device, which is an improvement of the particle inflow concept, does not require vacuum. This attribute simplifies the transformation procedure significantly and it makes rice transformation technology accessible to laboratories which may not have the resources to invest in more expensive particle bombardment instruments. We determined experimentally that we could recover transgenic rice plants utilizing three different particle bombardment instruments at comparable frequencies.
KW - Oryza saliva, particle bombardment, transformation, transgenic rice
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U2 - 10.1023/A:1008870012568
DO - 10.1023/A:1008870012568
M3 - Article
AN - SCOPUS:0031741635
VL - 7
SP - 289
EP - 294
JO - Transgenic Research
JF - Transgenic Research
SN - 0962-8819
IS - 4
ER -