An efficient rice transformation system utilizing mature seed-derived explants and a portable, inexpensive particle bombardment device

Durailagaraja Sudhakar, Le Tan Duc, Bui Ba Bong, Porntip Tinjuangjun, Shahina B. Maqbool, Marta Valdez, Richard Jefferson, Paul Christou

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

We developed a practical and efficient gene transfer system for indica rice utilizing mature-seed derived explants and a simple bombardment device which uses compressed helium for accelerating DNA-coated metal particles. Unlike instruments which have been described in the literature previously, this new bombardment device, which is an improvement of the particle inflow concept, does not require vacuum. This attribute simplifies the transformation procedure significantly and it makes rice transformation technology accessible to laboratories which may not have the resources to invest in more expensive particle bombardment instruments. We determined experimentally that we could recover transgenic rice plants utilizing three different particle bombardment instruments at comparable frequencies.

Original languageEnglish (US)
Pages (from-to)289-294
Number of pages6
JournalTransgenic Research
Volume7
Issue number4
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

Seeds
explants
rice
Equipment and Supplies
seeds
Helium
Genetically Modified Plants
Vacuum
helium
Metals
gene transfer
Technology
DNA
metals
genetically modified organisms
Genes
Oryza

Keywords

  • Oryza saliva, particle bombardment, transformation, transgenic rice

ASJC Scopus subject areas

  • Food Science
  • Plant Science
  • Biotechnology
  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology

Cite this

An efficient rice transformation system utilizing mature seed-derived explants and a portable, inexpensive particle bombardment device. / Sudhakar, Durailagaraja; Duc, Le Tan; Bong, Bui Ba; Tinjuangjun, Porntip; Maqbool, Shahina B.; Valdez, Marta; Jefferson, Richard; Christou, Paul.

In: Transgenic Research, Vol. 7, No. 4, 1998, p. 289-294.

Research output: Contribution to journalArticle

Sudhakar, Durailagaraja ; Duc, Le Tan ; Bong, Bui Ba ; Tinjuangjun, Porntip ; Maqbool, Shahina B. ; Valdez, Marta ; Jefferson, Richard ; Christou, Paul. / An efficient rice transformation system utilizing mature seed-derived explants and a portable, inexpensive particle bombardment device. In: Transgenic Research. 1998 ; Vol. 7, No. 4. pp. 289-294.
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