An efficient rice transformation system utilizing mature seed-derived explants and a portable, inexpensive particle bombardment device

Durailagaraja Sudhakar; Le Tan Duc; Bui Ba Bong; Porntip Tinjuangjun; Shahina Bano Maqbool; Marta Valdez; Richard Jefferson; Paul Christou

doi:10.1023/A:1008870012568

An efficient rice transformation system utilizing mature seed-derived explants and a portable, inexpensive particle bombardment device

Durailagaraja Sudhakar, Le Tan Duc, Bui Ba Bong, Porntip Tinjuangjun, Shahina Bano Maqbool, Marta Valdez, Richard Jefferson, Paul Christou

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

We developed a practical and efficient gene transfer system for indica rice utilizing mature-seed derived explants and a simple bombardment device which uses compressed helium for accelerating DNA-coated metal particles. Unlike instruments which have been described in the literature previously, this new bombardment device, which is an improvement of the particle inflow concept, does not require vacuum. This attribute simplifies the transformation procedure significantly and it makes rice transformation technology accessible to laboratories which may not have the resources to invest in more expensive particle bombardment instruments. We determined experimentally that we could recover transgenic rice plants utilizing three different particle bombardment instruments at comparable frequencies.

Original language	English (US)
Pages (from-to)	289-294
Number of pages	6
Journal	Transgenic Research
Volume	7
Issue number	4
DOIs	https://doi.org/10.1023/A:1008870012568
State	Published - Jan 1 1998
Externally published	Yes

Keywords

Oryza saliva, particle bombardment, transformation, transgenic rice

ASJC Scopus subject areas

Biotechnology
Animal Science and Zoology
Agronomy and Crop Science
Genetics

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An efficient rice transformation system utilizing mature seed-derived explants and a portable, inexpensive particle bombardment device. / Sudhakar, Durailagaraja; Duc, Le Tan; Bong, Bui Ba; Tinjuangjun, Porntip; Maqbool, Shahina Bano; Valdez, Marta; Jefferson, Richard; Christou, Paul.

In: Transgenic Research, Vol. 7, No. 4, 01.01.1998, p. 289-294.

Research output: Contribution to journal › Article › peer-review

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