An efficient rice transformation system utilizing mature seed-derived explants and a portable, inexpensive particle bombardment device

Durailagaraja Sudhakar, Le Tan Duc, Bui Ba Bong, Porntip Tinjuangjun, Shahina Bano Maqbool, Marta Valdez, Richard Jefferson, Paul Christou

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

We developed a practical and efficient gene transfer system for indica rice utilizing mature-seed derived explants and a simple bombardment device which uses compressed helium for accelerating DNA-coated metal particles. Unlike instruments which have been described in the literature previously, this new bombardment device, which is an improvement of the particle inflow concept, does not require vacuum. This attribute simplifies the transformation procedure significantly and it makes rice transformation technology accessible to laboratories which may not have the resources to invest in more expensive particle bombardment instruments. We determined experimentally that we could recover transgenic rice plants utilizing three different particle bombardment instruments at comparable frequencies.

Original languageEnglish (US)
Pages (from-to)289-294
Number of pages6
JournalTransgenic Research
Volume7
Issue number4
DOIs
Publication statusPublished - Jan 1 1998
Externally publishedYes

    Fingerprint

Keywords

  • Oryza saliva, particle bombardment, transformation, transgenic rice

ASJC Scopus subject areas

  • Biotechnology
  • Animal Science and Zoology
  • Agronomy and Crop Science
  • Genetics

Cite this