Abstract
We developed a practical and efficient gene transfer system for indica rice utilizing mature-seed derived explants and a simple bombardment device which uses compressed helium for accelerating DNA-coated metal particles. Unlike instruments which have been described in the literature previously, this new bombardment device, which is an improvement of the particle inflow concept, does not require vacuum. This attribute simplifies the transformation procedure significantly and it makes rice transformation technology accessible to laboratories which may not have the resources to invest in more expensive particle bombardment instruments. We determined experimentally that we could recover transgenic rice plants utilizing three different particle bombardment instruments at comparable frequencies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 289-294 |
| Number of pages | 6 |
| Journal | Transgenic Research |
| Volume | 7 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1998 |
| Externally published | Yes |
Fingerprint
Keywords
- Oryza saliva, particle bombardment, transformation, transgenic rice
ASJC Scopus subject areas
- Food Science
- Plant Science
- Biotechnology
- Genetics
- Molecular Biology
- Applied Microbiology and Biotechnology
Cite this
An efficient rice transformation system utilizing mature seed-derived explants and a portable, inexpensive particle bombardment device. / Sudhakar, Durailagaraja; Duc, Le Tan; Bong, Bui Ba; Tinjuangjun, Porntip; Maqbool, Shahina B.; Valdez, Marta; Jefferson, Richard; Christou, Paul.
In: Transgenic Research, Vol. 7, No. 4, 1998, p. 289-294.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An efficient rice transformation system utilizing mature seed-derived explants and a portable, inexpensive particle bombardment device
AU - Sudhakar, Durailagaraja
AU - Duc, Le Tan
AU - Bong, Bui Ba
AU - Tinjuangjun, Porntip
AU - Maqbool, Shahina B.
AU - Valdez, Marta
AU - Jefferson, Richard
AU - Christou, Paul
PY - 1998
Y1 - 1998
N2 - We developed a practical and efficient gene transfer system for indica rice utilizing mature-seed derived explants and a simple bombardment device which uses compressed helium for accelerating DNA-coated metal particles. Unlike instruments which have been described in the literature previously, this new bombardment device, which is an improvement of the particle inflow concept, does not require vacuum. This attribute simplifies the transformation procedure significantly and it makes rice transformation technology accessible to laboratories which may not have the resources to invest in more expensive particle bombardment instruments. We determined experimentally that we could recover transgenic rice plants utilizing three different particle bombardment instruments at comparable frequencies.
AB - We developed a practical and efficient gene transfer system for indica rice utilizing mature-seed derived explants and a simple bombardment device which uses compressed helium for accelerating DNA-coated metal particles. Unlike instruments which have been described in the literature previously, this new bombardment device, which is an improvement of the particle inflow concept, does not require vacuum. This attribute simplifies the transformation procedure significantly and it makes rice transformation technology accessible to laboratories which may not have the resources to invest in more expensive particle bombardment instruments. We determined experimentally that we could recover transgenic rice plants utilizing three different particle bombardment instruments at comparable frequencies.
KW - Oryza saliva, particle bombardment, transformation, transgenic rice
UR - http://www.scopus.com/inward/record.url?scp=0031741635&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031741635&partnerID=8YFLogxK
U2 - 10.1023/A:1008870012568
DO - 10.1023/A:1008870012568
M3 - Article
VL - 7
SP - 289
EP - 294
JO - Transgenic Research
JF - Transgenic Research
SN - 0962-8819
IS - 4
ER -