An efficient rice transformation system utilizing mature seed-derived explants and a portable, inexpensive particle bombardment device

Durailagaraja Sudhakar; Le Tan Duc; Bui Ba Bong; Porntip Tinjuangjun; Shahina Bano Maqbool; Marta Valdez; Richard Jefferson; Paul Christou

doi:10.1023/A:1008870012568

An efficient rice transformation system utilizing mature seed-derived explants and a portable, inexpensive particle bombardment device

Durailagaraja Sudhakar, Le Tan Duc, Bui Ba Bong, Porntip Tinjuangjun, Shahina Bano Maqbool, Marta Valdez, Richard Jefferson, Paul Christou

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

We developed a practical and efficient gene transfer system for indica rice utilizing mature-seed derived explants and a simple bombardment device which uses compressed helium for accelerating DNA-coated metal particles. Unlike instruments which have been described in the literature previously, this new bombardment device, which is an improvement of the particle inflow concept, does not require vacuum. This attribute simplifies the transformation procedure significantly and it makes rice transformation technology accessible to laboratories which may not have the resources to invest in more expensive particle bombardment instruments. We determined experimentally that we could recover transgenic rice plants utilizing three different particle bombardment instruments at comparable frequencies.

Original language	English (US)
Pages (from-to)	289-294
Number of pages	6
Journal	Transgenic Research
Volume	7
Issue number	4
DOIs	https://doi.org/10.1023/A:1008870012568
State	Published - 1998
Externally published	Yes

Keywords

Oryza saliva, particle bombardment, transformation, transgenic rice

ASJC Scopus subject areas

Biotechnology
Animal Science and Zoology
Agronomy and Crop Science
Genetics

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10.1023/A:1008870012568

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An efficient rice transformation system utilizing mature seed-derived explants and a portable, inexpensive particle bombardment device. / Sudhakar, Durailagaraja; Duc, Le Tan; Bong, Bui Ba; Tinjuangjun, Porntip; Maqbool, Shahina Bano; Valdez, Marta; Jefferson, Richard; Christou, Paul.

In: Transgenic Research, Vol. 7, No. 4, 1998, p. 289-294.

Research output: Contribution to journal › Article › peer-review

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abstract = "We developed a practical and efficient gene transfer system for indica rice utilizing mature-seed derived explants and a simple bombardment device which uses compressed helium for accelerating DNA-coated metal particles. Unlike instruments which have been described in the literature previously, this new bombardment device, which is an improvement of the particle inflow concept, does not require vacuum. This attribute simplifies the transformation procedure significantly and it makes rice transformation technology accessible to laboratories which may not have the resources to invest in more expensive particle bombardment instruments. We determined experimentally that we could recover transgenic rice plants utilizing three different particle bombardment instruments at comparable frequencies.",

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author = "Durailagaraja Sudhakar and Duc, {Le Tan} and Bong, {Bui Ba} and Porntip Tinjuangjun and Maqbool, {Shahina Bano} and Marta Valdez and Richard Jefferson and Paul Christou",

note = "Funding Information: We thank our colleagues at the JIC for useful discussions and support, in particular Dr Teresa Capell for advise with molecular analysis. This work was supported in part by a grant from the Rockefeller Foundation to P.C. M.V. is grateful to UNESCO for a visiting scientist fellowships at the JIC. D.S. and B.B.B. were the recipients of postdoctoral fellowships from the Rockefeller Foundation. L.T. Duc and S. Bano were supported through Dissertation fellowships from the Rockefeller Foundation. D. Sudhakar thanks Tamil Nadu Agric. Univ., Coimbatore, India, for Funding Information: support and cooperation. The JIC is supported by a grant in aid from the BBSRC, UK.",

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AU - Tinjuangjun, Porntip

AU - Maqbool, Shahina Bano

AU - Valdez, Marta

AU - Jefferson, Richard

AU - Christou, Paul

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PY - 1998

Y1 - 1998

N2 - We developed a practical and efficient gene transfer system for indica rice utilizing mature-seed derived explants and a simple bombardment device which uses compressed helium for accelerating DNA-coated metal particles. Unlike instruments which have been described in the literature previously, this new bombardment device, which is an improvement of the particle inflow concept, does not require vacuum. This attribute simplifies the transformation procedure significantly and it makes rice transformation technology accessible to laboratories which may not have the resources to invest in more expensive particle bombardment instruments. We determined experimentally that we could recover transgenic rice plants utilizing three different particle bombardment instruments at comparable frequencies.

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An efficient rice transformation system utilizing mature seed-derived explants and a portable, inexpensive particle bombardment device

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