An asymmetric NFAT1 dimer on a pseudo-palindromic κB-like DNA site

Lei Jin, Piotr Sliz, Lin Chen, Fernando Macian-Juan, Anjana Rao, Patrick G. Hogan, Stephen C. Harrison

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The crystal structure of the NFAT1 Rel homology region (RHR) bound to a pseudo-palindromic DNA site reveals an asymmetric dimer interaction between the RHR-C domains, unrelated to the contact seen in Rel dimers such as NFκB. Binding studies with a form of the NFAT1 RHR defective in the dimer contact show loss of cooperativity and demonstrate that the same interaction is present in solution. The structure we have determined may correspond to a functional NFAT binding mode at palindromic sites of genes induced during the anergic response to weak TCR signaling.

Original languageEnglish (US)
Pages (from-to)807-811
Number of pages5
JournalNature Structural Biology
Volume10
Issue number10
DOIs
StatePublished - Oct 1 2003

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Dimers
DNA
Genes
Crystal structure

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Genetics

Cite this

Jin, L., Sliz, P., Chen, L., Macian-Juan, F., Rao, A., Hogan, P. G., & Harrison, S. C. (2003). An asymmetric NFAT1 dimer on a pseudo-palindromic κB-like DNA site. Nature Structural Biology, 10(10), 807-811. https://doi.org/10.1038/nsb975

An asymmetric NFAT1 dimer on a pseudo-palindromic κB-like DNA site. / Jin, Lei; Sliz, Piotr; Chen, Lin; Macian-Juan, Fernando; Rao, Anjana; Hogan, Patrick G.; Harrison, Stephen C.

In: Nature Structural Biology, Vol. 10, No. 10, 01.10.2003, p. 807-811.

Research output: Contribution to journalArticle

Jin, L, Sliz, P, Chen, L, Macian-Juan, F, Rao, A, Hogan, PG & Harrison, SC 2003, 'An asymmetric NFAT1 dimer on a pseudo-palindromic κB-like DNA site', Nature Structural Biology, vol. 10, no. 10, pp. 807-811. https://doi.org/10.1038/nsb975
Jin, Lei ; Sliz, Piotr ; Chen, Lin ; Macian-Juan, Fernando ; Rao, Anjana ; Hogan, Patrick G. ; Harrison, Stephen C. / An asymmetric NFAT1 dimer on a pseudo-palindromic κB-like DNA site. In: Nature Structural Biology. 2003 ; Vol. 10, No. 10. pp. 807-811.
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