Amplification of the metallothionein-1 and metallothionein-2 genes in copper-resistant hepatoma cells

Mark J. Czaja, Francis R. Weiner, Jonathan H. Freedman

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The molecular basis tor increased metallothionein concentrations in copper-resistant hepatoma cells was examined. The copper-resistant cell line HAC600, which is maintained in 600 μm copper, had increased steady-state mRNA levels for both the metallothionein-1 (MT-1) and the metallothionein-2 (MT-2) genes. Levels of mRNA were increased 11-fold for MT-1 and 15-fold for MT-2, with no significant change in α-tubulin mRNA content. HAC600NM cells, which are copper-resistant cells kept in a normal copper concentration for over 1 year, also had eight- and tenfold increases in MT-1 and MT-2 mRNA levels. Nuclear run-on assays showed that MT-1 and MT-2 gene transcription was increased nine- and eightfold in HAC600 cells and seven- and tenfold in HAC600NM cells, respectively. Southern blot analysis showed amplification of both metallothionein genes in HAC600 and HAC600NM cells. Thus the molecular basis of increased metallothionein in these hepatoma cells involved a stable gene amplification of both MT genes. The greater increase in metallothionein mRNA levels in HAC600 cells relative to the changes in transcription suggests that posttranscriptional mechanisms of gene regulation may also be acting in these cells.

Original languageEnglish (US)
Pages (from-to)434-438
Number of pages5
JournalJournal of Cellular Physiology
Volume147
Issue number3
StatePublished - Jun 1991

Fingerprint

Metallothionein
Amplification
Copper
Hepatocellular Carcinoma
Genes
Messenger RNA
Transcription
Gene Amplification
Tubulin
Southern Blotting
Gene expression
Assays
Cells

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Amplification of the metallothionein-1 and metallothionein-2 genes in copper-resistant hepatoma cells. / Czaja, Mark J.; Weiner, Francis R.; Freedman, Jonathan H.

In: Journal of Cellular Physiology, Vol. 147, No. 3, 06.1991, p. 434-438.

Research output: Contribution to journalArticle

Czaja, Mark J. ; Weiner, Francis R. ; Freedman, Jonathan H. / Amplification of the metallothionein-1 and metallothionein-2 genes in copper-resistant hepatoma cells. In: Journal of Cellular Physiology. 1991 ; Vol. 147, No. 3. pp. 434-438.
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