Amplification of the biotin-avidin immunofluorescence technique

Joan W. Berman; Ross S. Basch

doi:10.1016/0022-1759(80)90138-6

Amplification of the biotin-avidin immunofluorescence technique

Joan W. Berman, Ross S. Basch

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

An amplification of the immunofluorescence technique which uses biotinylated antibody and fluoresceinated avidin is described. By introducing a sandwich technique using fluorescein-conjuated goat ant-avidin, a 5-fold enhancement of staining over the conventional immunofluorescence method is achieved, and the brightness is more than twice that achieved with the simple biotin-fluoresceinated avidin reaction.

Original language	English (US)
Pages (from-to)	335-338
Number of pages	4
Journal	Journal of Immunological Methods
Volume	36
Issue number	3-4
DOIs	https://doi.org/10.1016/0022-1759(80)90138-6
State	Published - 1980
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0022-1759(80)90138-6

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title = "Amplification of the biotin-avidin immunofluorescence technique",

abstract = "An amplification of the immunofluorescence technique which uses biotinylated antibody and fluoresceinated avidin is described. By introducing a sandwich technique using fluorescein-conjuated goat ant-avidin, a 5-fold enhancement of staining over the conventional immunofluorescence method is achieved, and the brightness is more than twice that achieved with the simple biotin-fluoresceinated avidin reaction.",

author = "Berman, {Joan W.} and Basch, {Ross S.}",

year = "1980",

doi = "10.1016/0022-1759(80)90138-6",

language = "English (US)",

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pages = "335--338",

journal = "Journal of Immunological Methods",

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T1 - Amplification of the biotin-avidin immunofluorescence technique

AU - Berman, Joan W.

AU - Basch, Ross S.

PY - 1980

Y1 - 1980

N2 - An amplification of the immunofluorescence technique which uses biotinylated antibody and fluoresceinated avidin is described. By introducing a sandwich technique using fluorescein-conjuated goat ant-avidin, a 5-fold enhancement of staining over the conventional immunofluorescence method is achieved, and the brightness is more than twice that achieved with the simple biotin-fluoresceinated avidin reaction.

AB - An amplification of the immunofluorescence technique which uses biotinylated antibody and fluoresceinated avidin is described. By introducing a sandwich technique using fluorescein-conjuated goat ant-avidin, a 5-fold enhancement of staining over the conventional immunofluorescence method is achieved, and the brightness is more than twice that achieved with the simple biotin-fluoresceinated avidin reaction.

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U2 - 10.1016/0022-1759(80)90138-6

DO - 10.1016/0022-1759(80)90138-6

M3 - Article

C2 - 7000912

AN - SCOPUS:0018904499

SN - 0022-1759

VL - 36

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EP - 338

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 3-4

ER -

Amplification of the biotin-avidin immunofluorescence technique

Abstract

ASJC Scopus subject areas

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