Amplification of the biotin-avidin immunofluorescence technique

Joan W. Berman, R. S. Basch

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

An amplification of the immunofluorescence technique which uses biotinylated antibody and fluoresceinated avidin is described. By introducing a sandwich technique using fluorescein-conjugated goat anti-avidin, a 5-fold enhancement of staining over the conventional immunofluorescence method is achieved, and the brightness is more than twice that achieved with the simple biotin-fluoresceinated avidin reaction.

Original languageEnglish (US)
Pages (from-to)335-338
Number of pages4
JournalJournal of Immunological Methods
Volume36
Issue number3-4
StatePublished - 1980
Externally publishedYes

Fingerprint

Avidin
Biotin
Fluorescent Antibody Technique
Fluorescein
Goats
Staining and Labeling
Antibodies

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Amplification of the biotin-avidin immunofluorescence technique. / Berman, Joan W.; Basch, R. S.

In: Journal of Immunological Methods, Vol. 36, No. 3-4, 1980, p. 335-338.

Research output: Contribution to journalArticle

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