Amniotic fluid concentrations of Δ 5 and Δ 4 steroids in fetuses with congenital adrenal hyperplasia due to 21-hydroxylase deficiency and in anencephalic fetuses

S. Pang, L. S. Levine, L. L. Cederqvist, M. Fuentes, V. M. Riccardi, J. H. Holcombe, H. M. Nitowsky, G. Sachs, C. E. Anderson, M. A. Duchon, R. Owens, Irwin R. Merkatz, M. I. New

Research output: Contribution to journalArticle

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Abstract

Amniotic fluid concentrations of the Δ 5 steroids [17-hydroxypregnenolone (Δ 5-17P) and dehydroepiandrosterone] and the Δ 4 steroids [17-hydroxyprogesterone (17-OHP), androstenedione (Δ 4-A), and testosterone (T)] of 77 normal pregnancies with gestational ages of 12 weeks to term were compared with those of 8 pregnancies at risk for congenital adrenal hyperplasia due to 21-hydroxylase deficiency (CAH) and 5 pregnancies with anencephalic fetuses. At midgestation (14, 14.5, and 17 weeks) in 3 fetuses at risk for CAH (1 male and 2 females), all of whom were later proven to be affected with CAH, the amniotic fluid hormonal concentrations compared to controls of the same sex showed elevated 17-OHP and Δ 4-A in all fetuses, elevated Δ 5-17P in the 2 females and upper normal level in the 1 male fetus, and elevated T in both females and upper normal level in the male. At term, concentrations of 17-OHP, Δ 4-A, Δ 5-17P, and T in a follow-up amniotic fluid sample in 1 female fetus with CAH were clearly elevated. In 5 other pregnancies at risk for CAH, all amniotic fluid steroids were completely within the normal range for their sex at both midgestation and term, and these fetuses were proven to be normal at birth (3 males and 2 females). In the four midgestational anencephalic fetuses (14.5, 16, 17, and 18 weeks), amniotic fluid concentrations of 17-OHP, and Δ 5-17P in both male and female fetuses and Δ 4-A in one female were below or in the lowest range of normal, while Δ 4-A and T in the male anencephalic fetuses did not clearly differ from normal. At term, the steroid concentrations in one female anencephalic amniotic fluid sample were all undetectable. However, these could not be distinguished from normal levels. The concentrations of dehydroepiandrosterone in midgestational or term amniotic fluid samples of CAH and anencephalic fetuses did not differ from normal values for the same sex. In conclusion, the decreased Δ 5 and Δ 4 17-hydroxylated steroids (Δ 5-17P and 17-OHP) in the amniotic fluid of anencephalic fetuses whose ACTH secretion is presumably decreased suggests that adrenal steroidogenesis is under ACTH regulation during midgestation. Further, the increased Δ 5 and Δ 4 17-hydroxylated steroid concentrations (Δ 5-17P, 17-OHP, and Δ 4-A) in the amniotic fluid of CAH fetuses suggests that ACTH secretion may be increased due to decreased cortisol synthesis in these fetuses and supports the concept that there is a functional feedback in the hypothalamic-pituitary-adrenocortical axis in the midgestational human fetus.

Original languageEnglish (US)
Pages (from-to)223-229
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume51
Issue number2
StatePublished - 1980
Externally publishedYes

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Steroid 21-Hydroxylase
Amniotic Fluid
Fetus
Steroids
Fluids
Adrenocorticotropic Hormone
Dehydroepiandrosterone
Pregnancy
Reference Values
17-alpha-Hydroxypregnenolone
17-alpha-Hydroxyprogesterone
Congenital adrenal hyperplasia due to 21 hydroxylase deficiency
Androstenedione
Hydrocortisone
Testosterone
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ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Amniotic fluid concentrations of Δ 5 and Δ 4 steroids in fetuses with congenital adrenal hyperplasia due to 21-hydroxylase deficiency and in anencephalic fetuses. / Pang, S.; Levine, L. S.; Cederqvist, L. L.; Fuentes, M.; Riccardi, V. M.; Holcombe, J. H.; Nitowsky, H. M.; Sachs, G.; Anderson, C. E.; Duchon, M. A.; Owens, R.; Merkatz, Irwin R.; New, M. I.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 51, No. 2, 1980, p. 223-229.

Research output: Contribution to journalArticle

Pang, S, Levine, LS, Cederqvist, LL, Fuentes, M, Riccardi, VM, Holcombe, JH, Nitowsky, HM, Sachs, G, Anderson, CE, Duchon, MA, Owens, R, Merkatz, IR & New, MI 1980, 'Amniotic fluid concentrations of Δ 5 and Δ 4 steroids in fetuses with congenital adrenal hyperplasia due to 21-hydroxylase deficiency and in anencephalic fetuses', Journal of Clinical Endocrinology and Metabolism, vol. 51, no. 2, pp. 223-229.
Pang, S. ; Levine, L. S. ; Cederqvist, L. L. ; Fuentes, M. ; Riccardi, V. M. ; Holcombe, J. H. ; Nitowsky, H. M. ; Sachs, G. ; Anderson, C. E. ; Duchon, M. A. ; Owens, R. ; Merkatz, Irwin R. ; New, M. I. / Amniotic fluid concentrations of Δ 5 and Δ 4 steroids in fetuses with congenital adrenal hyperplasia due to 21-hydroxylase deficiency and in anencephalic fetuses. In: Journal of Clinical Endocrinology and Metabolism. 1980 ; Vol. 51, No. 2. pp. 223-229.
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T1 - Amniotic fluid concentrations of Δ 5 and Δ 4 steroids in fetuses with congenital adrenal hyperplasia due to 21-hydroxylase deficiency and in anencephalic fetuses

AU - Pang, S.

AU - Levine, L. S.

AU - Cederqvist, L. L.

AU - Fuentes, M.

AU - Riccardi, V. M.

AU - Holcombe, J. H.

AU - Nitowsky, H. M.

AU - Sachs, G.

AU - Anderson, C. E.

AU - Duchon, M. A.

AU - Owens, R.

AU - Merkatz, Irwin R.

AU - New, M. I.

PY - 1980

Y1 - 1980

N2 - Amniotic fluid concentrations of the Δ 5 steroids [17-hydroxypregnenolone (Δ 5-17P) and dehydroepiandrosterone] and the Δ 4 steroids [17-hydroxyprogesterone (17-OHP), androstenedione (Δ 4-A), and testosterone (T)] of 77 normal pregnancies with gestational ages of 12 weeks to term were compared with those of 8 pregnancies at risk for congenital adrenal hyperplasia due to 21-hydroxylase deficiency (CAH) and 5 pregnancies with anencephalic fetuses. At midgestation (14, 14.5, and 17 weeks) in 3 fetuses at risk for CAH (1 male and 2 females), all of whom were later proven to be affected with CAH, the amniotic fluid hormonal concentrations compared to controls of the same sex showed elevated 17-OHP and Δ 4-A in all fetuses, elevated Δ 5-17P in the 2 females and upper normal level in the 1 male fetus, and elevated T in both females and upper normal level in the male. At term, concentrations of 17-OHP, Δ 4-A, Δ 5-17P, and T in a follow-up amniotic fluid sample in 1 female fetus with CAH were clearly elevated. In 5 other pregnancies at risk for CAH, all amniotic fluid steroids were completely within the normal range for their sex at both midgestation and term, and these fetuses were proven to be normal at birth (3 males and 2 females). In the four midgestational anencephalic fetuses (14.5, 16, 17, and 18 weeks), amniotic fluid concentrations of 17-OHP, and Δ 5-17P in both male and female fetuses and Δ 4-A in one female were below or in the lowest range of normal, while Δ 4-A and T in the male anencephalic fetuses did not clearly differ from normal. At term, the steroid concentrations in one female anencephalic amniotic fluid sample were all undetectable. However, these could not be distinguished from normal levels. The concentrations of dehydroepiandrosterone in midgestational or term amniotic fluid samples of CAH and anencephalic fetuses did not differ from normal values for the same sex. In conclusion, the decreased Δ 5 and Δ 4 17-hydroxylated steroids (Δ 5-17P and 17-OHP) in the amniotic fluid of anencephalic fetuses whose ACTH secretion is presumably decreased suggests that adrenal steroidogenesis is under ACTH regulation during midgestation. Further, the increased Δ 5 and Δ 4 17-hydroxylated steroid concentrations (Δ 5-17P, 17-OHP, and Δ 4-A) in the amniotic fluid of CAH fetuses suggests that ACTH secretion may be increased due to decreased cortisol synthesis in these fetuses and supports the concept that there is a functional feedback in the hypothalamic-pituitary-adrenocortical axis in the midgestational human fetus.

AB - Amniotic fluid concentrations of the Δ 5 steroids [17-hydroxypregnenolone (Δ 5-17P) and dehydroepiandrosterone] and the Δ 4 steroids [17-hydroxyprogesterone (17-OHP), androstenedione (Δ 4-A), and testosterone (T)] of 77 normal pregnancies with gestational ages of 12 weeks to term were compared with those of 8 pregnancies at risk for congenital adrenal hyperplasia due to 21-hydroxylase deficiency (CAH) and 5 pregnancies with anencephalic fetuses. At midgestation (14, 14.5, and 17 weeks) in 3 fetuses at risk for CAH (1 male and 2 females), all of whom were later proven to be affected with CAH, the amniotic fluid hormonal concentrations compared to controls of the same sex showed elevated 17-OHP and Δ 4-A in all fetuses, elevated Δ 5-17P in the 2 females and upper normal level in the 1 male fetus, and elevated T in both females and upper normal level in the male. At term, concentrations of 17-OHP, Δ 4-A, Δ 5-17P, and T in a follow-up amniotic fluid sample in 1 female fetus with CAH were clearly elevated. In 5 other pregnancies at risk for CAH, all amniotic fluid steroids were completely within the normal range for their sex at both midgestation and term, and these fetuses were proven to be normal at birth (3 males and 2 females). In the four midgestational anencephalic fetuses (14.5, 16, 17, and 18 weeks), amniotic fluid concentrations of 17-OHP, and Δ 5-17P in both male and female fetuses and Δ 4-A in one female were below or in the lowest range of normal, while Δ 4-A and T in the male anencephalic fetuses did not clearly differ from normal. At term, the steroid concentrations in one female anencephalic amniotic fluid sample were all undetectable. However, these could not be distinguished from normal levels. The concentrations of dehydroepiandrosterone in midgestational or term amniotic fluid samples of CAH and anencephalic fetuses did not differ from normal values for the same sex. In conclusion, the decreased Δ 5 and Δ 4 17-hydroxylated steroids (Δ 5-17P and 17-OHP) in the amniotic fluid of anencephalic fetuses whose ACTH secretion is presumably decreased suggests that adrenal steroidogenesis is under ACTH regulation during midgestation. Further, the increased Δ 5 and Δ 4 17-hydroxylated steroid concentrations (Δ 5-17P, 17-OHP, and Δ 4-A) in the amniotic fluid of CAH fetuses suggests that ACTH secretion may be increased due to decreased cortisol synthesis in these fetuses and supports the concept that there is a functional feedback in the hypothalamic-pituitary-adrenocortical axis in the midgestational human fetus.

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