Amino acid sequences of mouse 2.5S nerve growth factor. I. Isolation and characterization of the soluble tryptic and chymotryptic peptides

Ruth Hogue Angeletti, Delio Mercanti, Ralph A. Bradshaw

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The determination of the amino acid sequence of nerve growth factor (NGF), prepared as the 2.5S derivative from mouse submandibular glands, has been initiated. Tryptic digestion of S-carboxymethyl- and S-aminoethyl-NGF, followed by purification and characterization of the resulting peptides, was performed. These peptides accounted for 87 residues in the NGF molecule but contained only 5 of the 6 half-cystinyl residues and were missing the amino-terminal fragment. Digestion of the S-carboxymethyl derivative with chymotrypsin yielded 19 peptides covering 108 unique residues. By combined Edman degradation and carboxypeptidase A and B digestion, 96 of the 108 residues, accounted for by peptides from the three separate digests were placed in sequence.

Original languageEnglish (US)
Pages (from-to)90-100
Number of pages11
JournalBiochemistry
Volume12
Issue number1
StatePublished - 1973
Externally publishedYes

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Amino Acid Sequence
Nerve Growth Factor
Amino Acids
Digestion
Peptides
Carboxypeptidase B
Carboxypeptidases A
Derivatives
Submandibular Gland
Protein Sequence Analysis
Chymotrypsin
Purification
Degradation
Molecules
2.5S nerve growth factor

ASJC Scopus subject areas

  • Biochemistry

Cite this

Amino acid sequences of mouse 2.5S nerve growth factor. I. Isolation and characterization of the soluble tryptic and chymotryptic peptides. / Angeletti, Ruth Hogue; Mercanti, Delio; Bradshaw, Ralph A.

In: Biochemistry, Vol. 12, No. 1, 1973, p. 90-100.

Research output: Contribution to journalArticle

Angeletti, Ruth Hogue ; Mercanti, Delio ; Bradshaw, Ralph A. / Amino acid sequences of mouse 2.5S nerve growth factor. I. Isolation and characterization of the soluble tryptic and chymotryptic peptides. In: Biochemistry. 1973 ; Vol. 12, No. 1. pp. 90-100.
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