TY - JOUR
T1 - American tegumentary leishmaniasis
T2 - Antigen-gene polymorphism, taxonomy and clinical pleomorphism
AU - Garcia, A. L.
AU - Kindt, A.
AU - Quispe-Tintaya, K. W.
AU - Bermudez, H.
AU - Llanos, A.
AU - Arevalo, J.
AU - Bañuls, A. L.
AU - De Doncker, S.
AU - Le Ray, D.
AU - Dujardin, J. C.
N1 - Funding Information:
This study received financial support from TDR (Grant 00476), EC (Grant IC18-CT96-0123), Belgian technical cooperation (02-0001-BOL/00/003), the Belgian Agency for Cooperation and Development (DGOS) and FWO (Flemish fund for Scientific research 1.5.047.02). We thank Mrs. Vanessa Yardley for revising this article.
PY - 2005/3
Y1 - 2005/3
N2 - Multi-locus enzyme electrophoresis is the current gold standard for the genetic characterisation of Leishmania. However, this method is time-consuming and, more importantly, cannot be directly applied to parasites present in host tissue. PCR-based methods represent an ideal alternative but, to date, a multi-locus analysis has not been applied to the same sample. This has now been achieved with a sample of 55 neotropical isolates (Leishmania (Viannia) braziliensis, L. (V.) peruviana, L. (V.) guyanensis, L. (V.) lainsoni and L. (L.) amazonensis), using five different genes as targets, four of which encoded major Leishmania antigens (gp63, Hsp70, H2B and Cpb). Our multi-locus approach strongly supports the current taxonomy and demonstrates a highly robust method of distinguishing different strains. Within L. (V.) braziliensis, we did not encounter so far specific genetic differences between parasites isolated from cutaneous and mucosal lesions. Interestingly, results provided by each of the different antigen-genes in the species considered, were different, suggesting different selective pressures. Our work emphasises the need for a multi-disciplin ary approach to study the clinical pleomorphism of leishmaniasis.
AB - Multi-locus enzyme electrophoresis is the current gold standard for the genetic characterisation of Leishmania. However, this method is time-consuming and, more importantly, cannot be directly applied to parasites present in host tissue. PCR-based methods represent an ideal alternative but, to date, a multi-locus analysis has not been applied to the same sample. This has now been achieved with a sample of 55 neotropical isolates (Leishmania (Viannia) braziliensis, L. (V.) peruviana, L. (V.) guyanensis, L. (V.) lainsoni and L. (L.) amazonensis), using five different genes as targets, four of which encoded major Leishmania antigens (gp63, Hsp70, H2B and Cpb). Our multi-locus approach strongly supports the current taxonomy and demonstrates a highly robust method of distinguishing different strains. Within L. (V.) braziliensis, we did not encounter so far specific genetic differences between parasites isolated from cutaneous and mucosal lesions. Interestingly, results provided by each of the different antigen-genes in the species considered, were different, suggesting different selective pressures. Our work emphasises the need for a multi-disciplin ary approach to study the clinical pleomorphism of leishmaniasis.
KW - Antigens
KW - Muco-cutaneous leishmaniasis
KW - Multi-locus PCR
KW - Taxonomy
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U2 - 10.1016/j.meegid.2004.07.003
DO - 10.1016/j.meegid.2004.07.003
M3 - Article
C2 - 15639742
AN - SCOPUS:19944425914
SN - 1567-1348
VL - 5
SP - 109
EP - 116
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
IS - 2
ER -