American ginseng attenuates colitis-associated colon carcinogenesis in Mice

Impact on gut microbiota and metabolomics

Chong Zhi Wang, Chunhao Yu, Xiao Dong Wen, Lina Chen, Chun Feng Zhang, Tyler Calway, Yunping Qiu, Yunwei Wang, Zhiyu Zhang, Samantha Anderson, Yitao Wang, Wei Jia, Chun Su Yuan

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Inflammatory bowel disease is a risk factor for colorectal cancer initiation and development. In this study, the effects of American ginseng on chemically induced colitis and colon carcinogenesis were evaluated using an azoxymethane (AOM)/dextran sulfate sodium (DSS) mouse model. During the acute phase on day 15, the oral administration of ginseng (15 and 30 mg/kg/day) significantly suppressed AOM/DSS-induced colitis, as demonstrated by the disease activity index and colon tissue histology. During the chronic phase in week 13, AOM/DSS-induced tumor multiplicity was significantly suppressed by ginseng. Ginseng significantly attenuated the increase of inflammatory cytokines, such as IL1α, IL1β, IL6, G-CSF, and GM-CSF. Serum metabolomics data in the PCA plots showed good separation between the AOM/DSS model and ginseng-treated mice, and the most important endogenous metabolite changes were identified. The 16S rRNA data showed that after AOM/DSS, the microbiome community in the model group was obviously changed, and ginseng inhibited these changes. Fecal metabolomics analysis supported these findings. In conclusion, oral ginseng significantly decreased AOM/DSS- induced colitis and colon carcinogenesis by inhibiting inflammatory cytokines and restoring the metabolomics and microbiota profiles accordingly. Selective endogenous small molecules could be used as biomarkers to elucidate the effects of ginseng treatment.

Original languageEnglish (US)
Pages (from-to)803-811
Number of pages9
JournalCancer Prevention Research
Volume9
Issue number10
DOIs
StatePublished - Oct 1 2016

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Panax
Metabolomics
Azoxymethane
Colitis
Dextran Sulfate
Colon
Carcinogenesis
Microbiota
Granulocyte-Macrophage Colony-Stimulating Factor
Cytokines
Passive Cutaneous Anaphylaxis
Gastrointestinal Microbiome
Granulocyte Colony-Stimulating Factor
Inflammatory Bowel Diseases
Oral Administration
Colorectal Neoplasms
Interleukin-6
Histology
Biomarkers
Serum

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

American ginseng attenuates colitis-associated colon carcinogenesis in Mice : Impact on gut microbiota and metabolomics. / Wang, Chong Zhi; Yu, Chunhao; Wen, Xiao Dong; Chen, Lina; Zhang, Chun Feng; Calway, Tyler; Qiu, Yunping; Wang, Yunwei; Zhang, Zhiyu; Anderson, Samantha; Wang, Yitao; Jia, Wei; Yuan, Chun Su.

In: Cancer Prevention Research, Vol. 9, No. 10, 01.10.2016, p. 803-811.

Research output: Contribution to journalArticle

Wang, CZ, Yu, C, Wen, XD, Chen, L, Zhang, CF, Calway, T, Qiu, Y, Wang, Y, Zhang, Z, Anderson, S, Wang, Y, Jia, W & Yuan, CS 2016, 'American ginseng attenuates colitis-associated colon carcinogenesis in Mice: Impact on gut microbiota and metabolomics', Cancer Prevention Research, vol. 9, no. 10, pp. 803-811. https://doi.org/10.1158/1940-6207.CAPR-15-0372
Wang, Chong Zhi ; Yu, Chunhao ; Wen, Xiao Dong ; Chen, Lina ; Zhang, Chun Feng ; Calway, Tyler ; Qiu, Yunping ; Wang, Yunwei ; Zhang, Zhiyu ; Anderson, Samantha ; Wang, Yitao ; Jia, Wei ; Yuan, Chun Su. / American ginseng attenuates colitis-associated colon carcinogenesis in Mice : Impact on gut microbiota and metabolomics. In: Cancer Prevention Research. 2016 ; Vol. 9, No. 10. pp. 803-811.
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