Amelioration of insulin resistance but not hyperinsulinemia in obese mice overexpressing GLUT4 selectively in skeletal muscle

Tsu Shuen Tsao, Ellen B. Katz, David Pommer, Maureen J. Charron

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Abstract

The effects of gold-thioglucose (GTG) treatment were examined in mice overexpressing GLUT4 selectively in skeletal muscle (MLC-GLUT4 mice) and in age-matched controls. Groups of MLC-GLUT4 and control mice were injected with GTG or saline at 5 weeks of age. At 12 weeks following the injections, GTG- treated control mice exhibited a 35% increase in body weight versus saline- treated controls. Similarly, a 30% increase in body weight was observed in GTG-treated MLC-GLUT4 mice compared with saline-treated MLC-GLUT4 mice 12 weeks after the injections. In saline-treated lean MLC-GLUT4 and control mice, intraperitoneal injection of insulin decreased blood glucose in 1 hour by 63% and 38%, respectively. Insulin also decreased blood glucose by 40% in GTG-treated obese MLC-GLUT4 mice after 1 hour. However, insulin did not reduce blood glucose levels in GTG-treated obese control mice. The ability of insulin to clear blood glucose in GTG-treated obese MLC-GLUT4 mice is associated with increased skeletal muscle GLUT4 content and white adipose tissue (WAT) GLUT4 content as compared with GTG-treated obese controls. However, fasting blood glucose levels in GTG-treated obese MLC-GLUT4 and control mice were elevated by approximately 30% compared with saline-treated groups. Lastly, although GTG-treated obese MLC-GLUT4 mice exhibited improved glucose clearance in response to insulin, they nevertheless remained as hyperinsulinemic as GTG-treated obese control mice. These results suggest that genetic overexpression of GLUT4 in skeletal muscle may ameliorate the development of insulin resistance associated with obesity but cannot restore normal glucose and insulin levels. (C) 2000 by W.B. Saunders Company.

Original languageEnglish (US)
Pages (from-to)340-346
Number of pages7
JournalMetabolism: Clinical and Experimental
Volume49
Issue number3
StatePublished - 2000

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Aurothioglucose
Obese Mice
Hyperinsulinism
Insulin Resistance
Skeletal Muscle
Blood Glucose
Insulin
Body Weight
Glucose
White Adipose Tissue
Injections
Aptitude
Intraperitoneal Injections

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Amelioration of insulin resistance but not hyperinsulinemia in obese mice overexpressing GLUT4 selectively in skeletal muscle. / Tsao, Tsu Shuen; Katz, Ellen B.; Pommer, David; Charron, Maureen J.

In: Metabolism: Clinical and Experimental, Vol. 49, No. 3, 2000, p. 340-346.

Research output: Contribution to journalArticle

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