Amelioration of diabetes in mice after single-donor islet transplantation using the controlled release of gelatinized FGF-2

Jorge David Rivas-Carrillo, Nalu Navarro-Alvarez, Alejandro Soto-Gutierrez, Teru Okitsu, Yong Chen, Yasuhiko Tabata, Haruo Misawa, Hirofumi Noguchi, Shinichi Matsumoto, Noriaki Tanaka, Naoya Kobayashi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Fibroblast growth factor (FGF)-2 has been recognized to be a key element involved in angiogenesis and a putative factor involved in stem cell-mediated islet regeneration. However, the usefulness of FGF-2 in an islet transplantation setting has not yet been explored. We therefore evaluated the effect of FGF-2 on both islet culture and islet transplantation. Isolated islets were cultured in the presence of 100 ng/ml FGF-2 for a week and then the glucose-responding insulin secretion and insulin contents were measured. Gelatinized FGF-2 (100 ng), which allowed the controlled release of FGF-2, was used for islet transplantation of streptozotocin-induced diabetic mice. Islets (150 IEQ), obtained from a single donor, mixed with gelatinized FGF-2. were transplanted into the subrenal capsule of the mice and the animals were observed for 30 days. Revascularization around the islet grafts was examined. The blood glucose levels were measured and the intraperitoneal glucose tolerance test (IPGTT) was performed. The supplementation of FGF-2 maintained proper insulin secretion and insulin contents in an in vitro culture. The use of gelatinized FGF-2 facilitated revascularization and favorable islet engraftment, thus resulting in an amelioration of the blood glucose levels in diabetic mice. The utilization of FGF-2 showed increased contents of insulin in the islet grafts and revealed a similar pattern as that of normal healthy mice in IPGTT. In contrast, the transplantation of islets without FGF-2 supplementation showed poor revascularization and failed to control the blood glucose levels in the diabetic mice.

Original languageEnglish (US)
Pages (from-to)939-944
Number of pages6
JournalCell Transplantation
Volume15
Issue number10
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Islets of Langerhans Transplantation
Fibroblast Growth Factor 2
Fibroblasts
Medical problems
Insulin
Glucose
Blood Glucose
Blood
Glucose Tolerance Test
Cell culture
Grafts
Intercellular Signaling Peptides and Proteins
Transplants
Streptozocin
Stem cells
Capsules
Regeneration
Animals
Stem Cells

Keywords

  • Angiogenesis
  • Diabetes
  • FGF-2
  • Islet transplantation

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

Cite this

Rivas-Carrillo, J. D., Navarro-Alvarez, N., Soto-Gutierrez, A., Okitsu, T., Chen, Y., Tabata, Y., ... Kobayashi, N. (2006). Amelioration of diabetes in mice after single-donor islet transplantation using the controlled release of gelatinized FGF-2. Cell Transplantation, 15(10), 939-944. https://doi.org/10.3727/000000006783981323

Amelioration of diabetes in mice after single-donor islet transplantation using the controlled release of gelatinized FGF-2. / Rivas-Carrillo, Jorge David; Navarro-Alvarez, Nalu; Soto-Gutierrez, Alejandro; Okitsu, Teru; Chen, Yong; Tabata, Yasuhiko; Misawa, Haruo; Noguchi, Hirofumi; Matsumoto, Shinichi; Tanaka, Noriaki; Kobayashi, Naoya.

In: Cell Transplantation, Vol. 15, No. 10, 2006, p. 939-944.

Research output: Contribution to journalArticle

Rivas-Carrillo, JD, Navarro-Alvarez, N, Soto-Gutierrez, A, Okitsu, T, Chen, Y, Tabata, Y, Misawa, H, Noguchi, H, Matsumoto, S, Tanaka, N & Kobayashi, N 2006, 'Amelioration of diabetes in mice after single-donor islet transplantation using the controlled release of gelatinized FGF-2', Cell Transplantation, vol. 15, no. 10, pp. 939-944. https://doi.org/10.3727/000000006783981323
Rivas-Carrillo, Jorge David ; Navarro-Alvarez, Nalu ; Soto-Gutierrez, Alejandro ; Okitsu, Teru ; Chen, Yong ; Tabata, Yasuhiko ; Misawa, Haruo ; Noguchi, Hirofumi ; Matsumoto, Shinichi ; Tanaka, Noriaki ; Kobayashi, Naoya. / Amelioration of diabetes in mice after single-donor islet transplantation using the controlled release of gelatinized FGF-2. In: Cell Transplantation. 2006 ; Vol. 15, No. 10. pp. 939-944.
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AU - Okitsu, Teru

AU - Chen, Yong

AU - Tabata, Yasuhiko

AU - Misawa, Haruo

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