Alternate splicing of mRNAs encoding human mast cell growth factor and localization of the gene to chromosome 12q22-q24.

D. M. Anderson, D. E. Williams, R. Tushinski, S. Gimpel, J. Eisenman, L. A. Cannizzaro, M. Aronson, C. M. Croce, K. Huebner, D. Cosman

Research output: Contribution to journalArticle

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Abstract

Human mast cell growth factor (MGF) complementary DNAs (cDNAs) were cloned from HeLa cells using the polymerase chain reaction with oligonucleotides corresponding to murine and human MGF sequences. Sequencing of the cloned human MGF polymerase chain reaction products revealed two types of cDNA: a full length form corresponding in size to the murine cDNA, and an alternately spliced clone with a deletion of the sixth exon of the gene. Since membrane-bound MGF is predicted to be proteolytically cleaved within the sequences encoded by exon 6 to generate a soluble protein, this alternately spliced cDNA would likely encode a noncleavable, membrane-bound form of MGF. No difference in biological activity on human bone marrow cells was observed with recombinant, soluble forms of both types of human MGF protein. Our previous localization of the murine MGF gene to the Sl locus on chromosome 10 suggested (via conserved linkage groups) that the human MGF gene would be located on human chromosome 12. Therefore, rodent-human somatic cell hybrids with or without an entire human chromosome 12 and hybrids retaining partial 12 were tested by Southern blot analysis and used to show the presence of the human Mgf locus at chromosome region 12q. Chromosomal in situ hybridization localized the gene to 12q22-q24 in the region predicted by the comparative mapping of the murine Mgf/Sl locus.

Original languageEnglish (US)
Pages (from-to)373-378
Number of pages6
JournalCell growth & differentiation : the molecular biology journal of the American Association for Cancer Research
Volume2
Issue number8
StatePublished - Aug 1991
Externally publishedYes

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Stem Cell Factor
Alternative Splicing
Chromosomes
Messenger RNA
Genes
Complementary DNA
Chromosomes, Human, Pair 12
Human Chromosomes
Exons
Polymerase Chain Reaction
Chromosomes, Human, Pair 10
Membranes
Hybrid Cells
Southern Blotting
HeLa Cells
Human Activities
Oligonucleotides
Bone Marrow Cells
In Situ Hybridization
Rodentia

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Alternate splicing of mRNAs encoding human mast cell growth factor and localization of the gene to chromosome 12q22-q24. / Anderson, D. M.; Williams, D. E.; Tushinski, R.; Gimpel, S.; Eisenman, J.; Cannizzaro, L. A.; Aronson, M.; Croce, C. M.; Huebner, K.; Cosman, D.

In: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 2, No. 8, 08.1991, p. 373-378.

Research output: Contribution to journalArticle

Anderson, DM, Williams, DE, Tushinski, R, Gimpel, S, Eisenman, J, Cannizzaro, LA, Aronson, M, Croce, CM, Huebner, K & Cosman, D 1991, 'Alternate splicing of mRNAs encoding human mast cell growth factor and localization of the gene to chromosome 12q22-q24.', Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, vol. 2, no. 8, pp. 373-378.
Anderson, D. M. ; Williams, D. E. ; Tushinski, R. ; Gimpel, S. ; Eisenman, J. ; Cannizzaro, L. A. ; Aronson, M. ; Croce, C. M. ; Huebner, K. ; Cosman, D. / Alternate splicing of mRNAs encoding human mast cell growth factor and localization of the gene to chromosome 12q22-q24. In: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research. 1991 ; Vol. 2, No. 8. pp. 373-378.
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