Altered somatic hypermutation and reduced class-switch recombination in exonuclease 1-mutant mice

Philip D. Bardwell, Caroline J. Woo, Kaichun Wei, Ziqiang Li, Alberto Martin, Stephen Z. Sack, Tchaiko Parris, Winfried Edelmann, Matthew D. Scharff

Research output: Contribution to journalArticle

195 Citations (Scopus)

Abstract

The generation of protective antibodies requires somatic hypermutation (SHM) and class-switch recombination (CSR) of immunoglobulin genes. Here we show that mice mutant for exonuclease 1 (Exo1), which participates in DNA mismatch repair (MMR), have decreased CSR and changes in the characteristics of SHM similar to those previously observed in mice mutant for the MMR protein Msh2. Exo1 is thus the first exonuclease shown to be involved in SHM and CSR. The phenotype of Exo1-/- mice and the finding that Exo1 and Mlh1 are physically associated with mutating variable regions support the idea that Exo1 and MMR participate directly in SHM and CSR.

Original languageEnglish (US)
Pages (from-to)224-229
Number of pages6
JournalNature Immunology
Volume5
Issue number2
DOIs
StatePublished - Feb 2004

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Genetic Recombination
DNA Mismatch Repair
Exonucleases
Immunoglobulin Genes
Phenotype
Mouse Exo1 protein
Antibodies
exodeoxyribonuclease I
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Altered somatic hypermutation and reduced class-switch recombination in exonuclease 1-mutant mice. / Bardwell, Philip D.; Woo, Caroline J.; Wei, Kaichun; Li, Ziqiang; Martin, Alberto; Sack, Stephen Z.; Parris, Tchaiko; Edelmann, Winfried; Scharff, Matthew D.

In: Nature Immunology, Vol. 5, No. 2, 02.2004, p. 224-229.

Research output: Contribution to journalArticle

Bardwell, Philip D. ; Woo, Caroline J. ; Wei, Kaichun ; Li, Ziqiang ; Martin, Alberto ; Sack, Stephen Z. ; Parris, Tchaiko ; Edelmann, Winfried ; Scharff, Matthew D. / Altered somatic hypermutation and reduced class-switch recombination in exonuclease 1-mutant mice. In: Nature Immunology. 2004 ; Vol. 5, No. 2. pp. 224-229.
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