Altered mRNA level of matrix metalloproteinase-13 in MH7A synovial cells under mechanical loading and unloading

Hui (Herb) Sun, H. Yokota

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In an effort to elucidate the interplay between mechanical load and proteolytic gene expression in arthritic tissue degradation, we investigated cellular morphology and mRNA levels of matrix metalloproteinase-13 (MMP-13) genes under mechanical stress in human MH7A synovial cells. The cells were isolated from the knee joint of a rheumatoid arthritis patient. Using a reverse transcription-polymerase chain reaction procedure, we found that loading by an oscillatory shaker transiently decreased the level of MMP-13 mRNA and unloading by a clinostat increased its mRNA level. The unloaded cells appeared to be rounded and displayed a poorly developed track of peripheral fibers, whereas the cells under loading tended to align to the shear flow and were elongated. We also found that altering the oscillatory direction of mechanical loads contributed to a further reduction in mRNA expression of MMP-13. Our results demonstrate the role of mechanical loading and unloading in the transcriptional regulation of MMP-13 in synovial cells, and suggest the potential value of physical therapy for arthritic joints.

Original languageEnglish (US)
Pages (from-to)399-403
Number of pages5
JournalBone
Volume28
Issue number4
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Matrix Metalloproteinase 13
Messenger RNA
Arthritis
Mechanical Stress
Knee Joint
Reverse Transcription
Rheumatoid Arthritis
Joints
Gene Expression
Polymerase Chain Reaction
Genes

Keywords

  • Arthritis
  • Matrix metalloproteinase (MMP)
  • Mechanical loads
  • MH7A synovial cells
  • MMP-13
  • Transcription

ASJC Scopus subject areas

  • Physiology
  • Hematology

Cite this

Altered mRNA level of matrix metalloproteinase-13 in MH7A synovial cells under mechanical loading and unloading. / Sun, Hui (Herb); Yokota, H.

In: Bone, Vol. 28, No. 4, 2001, p. 399-403.

Research output: Contribution to journalArticle

@article{8c8b3443f1054612b75d5617d0805ee8,
title = "Altered mRNA level of matrix metalloproteinase-13 in MH7A synovial cells under mechanical loading and unloading",
abstract = "In an effort to elucidate the interplay between mechanical load and proteolytic gene expression in arthritic tissue degradation, we investigated cellular morphology and mRNA levels of matrix metalloproteinase-13 (MMP-13) genes under mechanical stress in human MH7A synovial cells. The cells were isolated from the knee joint of a rheumatoid arthritis patient. Using a reverse transcription-polymerase chain reaction procedure, we found that loading by an oscillatory shaker transiently decreased the level of MMP-13 mRNA and unloading by a clinostat increased its mRNA level. The unloaded cells appeared to be rounded and displayed a poorly developed track of peripheral fibers, whereas the cells under loading tended to align to the shear flow and were elongated. We also found that altering the oscillatory direction of mechanical loads contributed to a further reduction in mRNA expression of MMP-13. Our results demonstrate the role of mechanical loading and unloading in the transcriptional regulation of MMP-13 in synovial cells, and suggest the potential value of physical therapy for arthritic joints.",
keywords = "Arthritis, Matrix metalloproteinase (MMP), Mechanical loads, MH7A synovial cells, MMP-13, Transcription",
author = "Sun, {Hui (Herb)} and H. Yokota",
year = "2001",
doi = "10.1016/S8756-3282(00)00459-2",
language = "English (US)",
volume = "28",
pages = "399--403",
journal = "Bone",
issn = "8756-3282",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Altered mRNA level of matrix metalloproteinase-13 in MH7A synovial cells under mechanical loading and unloading

AU - Sun, Hui (Herb)

AU - Yokota, H.

PY - 2001

Y1 - 2001

N2 - In an effort to elucidate the interplay between mechanical load and proteolytic gene expression in arthritic tissue degradation, we investigated cellular morphology and mRNA levels of matrix metalloproteinase-13 (MMP-13) genes under mechanical stress in human MH7A synovial cells. The cells were isolated from the knee joint of a rheumatoid arthritis patient. Using a reverse transcription-polymerase chain reaction procedure, we found that loading by an oscillatory shaker transiently decreased the level of MMP-13 mRNA and unloading by a clinostat increased its mRNA level. The unloaded cells appeared to be rounded and displayed a poorly developed track of peripheral fibers, whereas the cells under loading tended to align to the shear flow and were elongated. We also found that altering the oscillatory direction of mechanical loads contributed to a further reduction in mRNA expression of MMP-13. Our results demonstrate the role of mechanical loading and unloading in the transcriptional regulation of MMP-13 in synovial cells, and suggest the potential value of physical therapy for arthritic joints.

AB - In an effort to elucidate the interplay between mechanical load and proteolytic gene expression in arthritic tissue degradation, we investigated cellular morphology and mRNA levels of matrix metalloproteinase-13 (MMP-13) genes under mechanical stress in human MH7A synovial cells. The cells were isolated from the knee joint of a rheumatoid arthritis patient. Using a reverse transcription-polymerase chain reaction procedure, we found that loading by an oscillatory shaker transiently decreased the level of MMP-13 mRNA and unloading by a clinostat increased its mRNA level. The unloaded cells appeared to be rounded and displayed a poorly developed track of peripheral fibers, whereas the cells under loading tended to align to the shear flow and were elongated. We also found that altering the oscillatory direction of mechanical loads contributed to a further reduction in mRNA expression of MMP-13. Our results demonstrate the role of mechanical loading and unloading in the transcriptional regulation of MMP-13 in synovial cells, and suggest the potential value of physical therapy for arthritic joints.

KW - Arthritis

KW - Matrix metalloproteinase (MMP)

KW - Mechanical loads

KW - MH7A synovial cells

KW - MMP-13

KW - Transcription

UR - http://www.scopus.com/inward/record.url?scp=0035032044&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035032044&partnerID=8YFLogxK

U2 - 10.1016/S8756-3282(00)00459-2

DO - 10.1016/S8756-3282(00)00459-2

M3 - Article

VL - 28

SP - 399

EP - 403

JO - Bone

JF - Bone

SN - 8756-3282

IS - 4

ER -