Altered mRNA level of matrix metalloproteinase-13 in MH7A synovial cells under mechanical loading and unloading

H. B. Sun, H. Yokota

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

In an effort to elucidate the interplay between mechanical load and proteolytic gene expression in arthritic tissue degradation, we investigated cellular morphology and mRNA levels of matrix metalloproteinase-13 (MMP-13) genes under mechanical stress in human MH7A synovial cells. The cells were isolated from the knee joint of a rheumatoid arthritis patient. Using a reverse transcription-polymerase chain reaction procedure, we found that loading by an oscillatory shaker transiently decreased the level of MMP-13 mRNA and unloading by a clinostat increased its mRNA level. The unloaded cells appeared to be rounded and displayed a poorly developed track of peripheral fibers, whereas the cells under loading tended to align to the shear flow and were elongated. We also found that altering the oscillatory direction of mechanical loads contributed to a further reduction in mRNA expression of MMP-13. Our results demonstrate the role of mechanical loading and unloading in the transcriptional regulation of MMP-13 in synovial cells, and suggest the potential value of physical therapy for arthritic joints.

Original languageEnglish (US)
Pages (from-to)399-403
Number of pages5
JournalBone
Volume28
Issue number4
DOIs
Publication statusPublished - May 10 2001
Externally publishedYes

    Fingerprint

Keywords

  • Arthritis
  • MH7A synovial cells
  • MMP-13
  • Matrix metalloproteinase (MMP)
  • Mechanical loads
  • Transcription

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Histology

Cite this