Altered biomarkers of mucosal immunity and reduced vaginal lactobacillus concentrations in sexually active female adolescents

Rebecca Pellett Madan, Colleen Carpenter, Tina Fiedler, Sabah Kalyoussef, Thomas C. McAndrew, Shankar Viswanathan, Mimi Kim, Marla J. Keller, David N. Fredricks, Betsy Herold

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background: Genital secretions collected from adult women exhibit in vitro activity against herpes simplex virus (HSV) and Escherichia coli (E. coli), but prior studies have not investigated this endogenous antimicrobial activity or its mediators in adolescent females. Methodology/Principal Findings: Anti-HSV and anti-E.coli activity were quantified from cervicovaginal lavage (CVL) specimens collected from 20 sexually active adolescent females (15-18 years). Soluble immune mediators that may influence this activity were measured in CVL, and concentrations of Lactobacillus jensenii and crispatus were quantified by PCR from vaginal swabs. Results for adolescents were compared to those obtained from 54 healthy, premenopausal adult women. Relative to specimens collected from adults, CVL collected from adolescent subjects had significantly reduced activity against E. coli and diminished concentrations of protein, IgG, and IgA but significantly increased anti-HSV activity and concentrations of interleukin (IL)-1α, IL-6 and IL-1 receptor antagonist. Vaginal swabs collected from adolescent subjects had comparable concentrations of L. crispatus but significantly reduced concentrations of L. jensenii, relative to adult swabs. Conclusions/Significance: Biomarkers of genital mucosal innate immunity may differ substantially between sexually active adolescents and adult women. These findings warrant further study and may have significant implications for prevention of sexually transmitted infections in adolescent females.

Original languageEnglish (US)
Article numbere40415
JournalPLoS One
Volume7
Issue number7
DOIs
StatePublished - Jul 10 2012

Fingerprint

mucosal immunity
Mucosal Immunity
Lactobacillus
Biomarkers
Viruses
Escherichia coli
biomarkers
herpes simplex
Lactobacillus jensenii
Therapeutic Irrigation
Simplexvirus
Interleukin-1 Receptors
Interleukin-1
interleukin-1
Immunoglobulin A
Interleukin-6
viruses
genitalia
Immunoglobulin G
Lactobacillus crispatus

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Altered biomarkers of mucosal immunity and reduced vaginal lactobacillus concentrations in sexually active female adolescents. / Madan, Rebecca Pellett; Carpenter, Colleen; Fiedler, Tina; Kalyoussef, Sabah; McAndrew, Thomas C.; Viswanathan, Shankar; Kim, Mimi; Keller, Marla J.; Fredricks, David N.; Herold, Betsy.

In: PLoS One, Vol. 7, No. 7, e40415, 10.07.2012.

Research output: Contribution to journalArticle

Madan, Rebecca Pellett ; Carpenter, Colleen ; Fiedler, Tina ; Kalyoussef, Sabah ; McAndrew, Thomas C. ; Viswanathan, Shankar ; Kim, Mimi ; Keller, Marla J. ; Fredricks, David N. ; Herold, Betsy. / Altered biomarkers of mucosal immunity and reduced vaginal lactobacillus concentrations in sexually active female adolescents. In: PLoS One. 2012 ; Vol. 7, No. 7.
@article{95558f5af99744b49751d39a6e455811,
title = "Altered biomarkers of mucosal immunity and reduced vaginal lactobacillus concentrations in sexually active female adolescents",
abstract = "Background: Genital secretions collected from adult women exhibit in vitro activity against herpes simplex virus (HSV) and Escherichia coli (E. coli), but prior studies have not investigated this endogenous antimicrobial activity or its mediators in adolescent females. Methodology/Principal Findings: Anti-HSV and anti-E.coli activity were quantified from cervicovaginal lavage (CVL) specimens collected from 20 sexually active adolescent females (15-18 years). Soluble immune mediators that may influence this activity were measured in CVL, and concentrations of Lactobacillus jensenii and crispatus were quantified by PCR from vaginal swabs. Results for adolescents were compared to those obtained from 54 healthy, premenopausal adult women. Relative to specimens collected from adults, CVL collected from adolescent subjects had significantly reduced activity against E. coli and diminished concentrations of protein, IgG, and IgA but significantly increased anti-HSV activity and concentrations of interleukin (IL)-1α, IL-6 and IL-1 receptor antagonist. Vaginal swabs collected from adolescent subjects had comparable concentrations of L. crispatus but significantly reduced concentrations of L. jensenii, relative to adult swabs. Conclusions/Significance: Biomarkers of genital mucosal innate immunity may differ substantially between sexually active adolescents and adult women. These findings warrant further study and may have significant implications for prevention of sexually transmitted infections in adolescent females.",
author = "Madan, {Rebecca Pellett} and Colleen Carpenter and Tina Fiedler and Sabah Kalyoussef and McAndrew, {Thomas C.} and Shankar Viswanathan and Mimi Kim and Keller, {Marla J.} and Fredricks, {David N.} and Betsy Herold",
year = "2012",
month = "7",
day = "10",
doi = "10.1371/journal.pone.0040415",
language = "English (US)",
volume = "7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "7",

}

TY - JOUR

T1 - Altered biomarkers of mucosal immunity and reduced vaginal lactobacillus concentrations in sexually active female adolescents

AU - Madan, Rebecca Pellett

AU - Carpenter, Colleen

AU - Fiedler, Tina

AU - Kalyoussef, Sabah

AU - McAndrew, Thomas C.

AU - Viswanathan, Shankar

AU - Kim, Mimi

AU - Keller, Marla J.

AU - Fredricks, David N.

AU - Herold, Betsy

PY - 2012/7/10

Y1 - 2012/7/10

N2 - Background: Genital secretions collected from adult women exhibit in vitro activity against herpes simplex virus (HSV) and Escherichia coli (E. coli), but prior studies have not investigated this endogenous antimicrobial activity or its mediators in adolescent females. Methodology/Principal Findings: Anti-HSV and anti-E.coli activity were quantified from cervicovaginal lavage (CVL) specimens collected from 20 sexually active adolescent females (15-18 years). Soluble immune mediators that may influence this activity were measured in CVL, and concentrations of Lactobacillus jensenii and crispatus were quantified by PCR from vaginal swabs. Results for adolescents were compared to those obtained from 54 healthy, premenopausal adult women. Relative to specimens collected from adults, CVL collected from adolescent subjects had significantly reduced activity against E. coli and diminished concentrations of protein, IgG, and IgA but significantly increased anti-HSV activity and concentrations of interleukin (IL)-1α, IL-6 and IL-1 receptor antagonist. Vaginal swabs collected from adolescent subjects had comparable concentrations of L. crispatus but significantly reduced concentrations of L. jensenii, relative to adult swabs. Conclusions/Significance: Biomarkers of genital mucosal innate immunity may differ substantially between sexually active adolescents and adult women. These findings warrant further study and may have significant implications for prevention of sexually transmitted infections in adolescent females.

AB - Background: Genital secretions collected from adult women exhibit in vitro activity against herpes simplex virus (HSV) and Escherichia coli (E. coli), but prior studies have not investigated this endogenous antimicrobial activity or its mediators in adolescent females. Methodology/Principal Findings: Anti-HSV and anti-E.coli activity were quantified from cervicovaginal lavage (CVL) specimens collected from 20 sexually active adolescent females (15-18 years). Soluble immune mediators that may influence this activity were measured in CVL, and concentrations of Lactobacillus jensenii and crispatus were quantified by PCR from vaginal swabs. Results for adolescents were compared to those obtained from 54 healthy, premenopausal adult women. Relative to specimens collected from adults, CVL collected from adolescent subjects had significantly reduced activity against E. coli and diminished concentrations of protein, IgG, and IgA but significantly increased anti-HSV activity and concentrations of interleukin (IL)-1α, IL-6 and IL-1 receptor antagonist. Vaginal swabs collected from adolescent subjects had comparable concentrations of L. crispatus but significantly reduced concentrations of L. jensenii, relative to adult swabs. Conclusions/Significance: Biomarkers of genital mucosal innate immunity may differ substantially between sexually active adolescents and adult women. These findings warrant further study and may have significant implications for prevention of sexually transmitted infections in adolescent females.

UR - http://www.scopus.com/inward/record.url?scp=84863713414&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863713414&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0040415

DO - 10.1371/journal.pone.0040415

M3 - Article

C2 - 22808157

AN - SCOPUS:84863713414

VL - 7

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 7

M1 - e40415

ER -