Alterations in the expression of colony-stimulating factor-1 and its receptor during an acute graft-vs-host reaction in mice

V. Praloran, C. Raventos-Suarez, A. Bartocci, J. Lucas, E. R. Stanley, J. J. Gibbons

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Abstract

During the course of an acute graft-vs-host-reaction in the mouse we observed a progressive increase in the concentration of CSF-1 in serum and liver, peaking at day 14. In contrast, there was a progressive decrease in the splenic CSF-1 concentration. In vivo studies of 125I-CSF-1 uptake and degradation and in vitro studies of 125I-CSF-1 binding by splenic cells demonstrated that whithin 24 h of the reaction the number of CSF-1 receptor+ cells had increased by 2-fold and their capacity to express the CSF-1 receptor by ~3-fold, resulting in a ~2.5-fold increase in the splenic clearance of CSF-1 from the circulation. Inasmuch as at 24 h, serum CSF-1 was not significantly altered, these results are suggestive of an increased rate of release of CSF-1 into the circulation early in the response. The splenic CSF-1-receptor bearing cells were in a Mac-1+ fraction that is consistent with a role for CSF-1 in the generation of host-derived splenic macrophages in acute graft-vs-host reaction.

Original languageEnglish (US)
Pages (from-to)3256-3261
Number of pages6
JournalJournal of Immunology
Volume145
Issue number10
Publication statusPublished - Jan 1 1990

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Praloran, V., Raventos-Suarez, C., Bartocci, A., Lucas, J., Stanley, E. R., & Gibbons, J. J. (1990). Alterations in the expression of colony-stimulating factor-1 and its receptor during an acute graft-vs-host reaction in mice. Journal of Immunology, 145(10), 3256-3261.