Alterations in the expression of colony-stimulating factor-1 and its receptor during an acute graft-vs-host reaction in mice

Vincent Praloran, Carmen Raventos-Suarez, Anna Bartocci, Judy Lucas, E. Richard Stanley, James J. Gibbons

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Abstract

During the course of an acute graft-vs-host reaction in the mouse we observed a progressive increase in the concentration of CSF-1 in serum and liver, peaking at day 14. In contrast, there was a progressive decrease in the splenic CSF-1 concentration. In vivo studies of 125I-CSF-1 uptake and degradation and in vitro studies of 125I-CSF-1 binding by splenic cells demonstrated that within 24 h of the reaction the number of CSF-1 receptor+ cells had increased by 2-fold and their capacity to express the CSF-1 receptor by ∼3-fold, resulting in a ∼2.5-fold increase in the splenic clearance of CSF-1 from the circulation. Inasmuch as at 24 h, serum CSF-1 was not significantly altered, these results are suggestive of an increased rate of release of CSF-1 into the circulation early in the response. The splenic CSF-1-receptor bearing cells were in a Mac-1+ fraction that is consistent with a role for CSF-1 in the generation of host-derived splenic macrophages in acute graft-vs-host reaction.

Original languageEnglish (US)
Pages (from-to)3256-3261
Number of pages6
JournalJournal of Immunology
Volume145
Issue number10
StatePublished - Nov 15 1990

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Graft vs Host Reaction
Macrophage Colony-Stimulating Factor
Macrophage Colony-Stimulating Factor Receptors
Serum
Macrophages

ASJC Scopus subject areas

  • Immunology

Cite this

Praloran, V., Raventos-Suarez, C., Bartocci, A., Lucas, J., Stanley, E. R., & Gibbons, J. J. (1990). Alterations in the expression of colony-stimulating factor-1 and its receptor during an acute graft-vs-host reaction in mice. Journal of Immunology, 145(10), 3256-3261.

Alterations in the expression of colony-stimulating factor-1 and its receptor during an acute graft-vs-host reaction in mice. / Praloran, Vincent; Raventos-Suarez, Carmen; Bartocci, Anna; Lucas, Judy; Stanley, E. Richard; Gibbons, James J.

In: Journal of Immunology, Vol. 145, No. 10, 15.11.1990, p. 3256-3261.

Research output: Contribution to journalArticle

Praloran, V, Raventos-Suarez, C, Bartocci, A, Lucas, J, Stanley, ER & Gibbons, JJ 1990, 'Alterations in the expression of colony-stimulating factor-1 and its receptor during an acute graft-vs-host reaction in mice', Journal of Immunology, vol. 145, no. 10, pp. 3256-3261.
Praloran V, Raventos-Suarez C, Bartocci A, Lucas J, Stanley ER, Gibbons JJ. Alterations in the expression of colony-stimulating factor-1 and its receptor during an acute graft-vs-host reaction in mice. Journal of Immunology. 1990 Nov 15;145(10):3256-3261.
Praloran, Vincent ; Raventos-Suarez, Carmen ; Bartocci, Anna ; Lucas, Judy ; Stanley, E. Richard ; Gibbons, James J. / Alterations in the expression of colony-stimulating factor-1 and its receptor during an acute graft-vs-host reaction in mice. In: Journal of Immunology. 1990 ; Vol. 145, No. 10. pp. 3256-3261.
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