Abstract
Chagas disease, characterized by acute myocarditis and chronic cardiomyopathy, is caused by infection with the protozoan parasite Trypanosoma cruzi. We sought to identify genes altered during the development of parasite-induced cardiomyopathy. Microarrays containing 27,400 sequence-verified mouse cDNAs were used to analyze global gene expression changes in the myocardium of a murine model of chagasic cardiomyopathy. Changes in gene expression were determined as the acute stage of infection developed into the chronic stage. This analysis was performed on the hearts of male CD-1 mice infected with trypomastigotes of T. cruzi (Brazil strain). At each interval we compared infected and uninfected mice and confirmed the microarray data with dye reversal. We identified eight distinct categories of mRNAs that were differentially regulated during infection and identified dysregulation of several key genes. These data may provide insight into the pathogenesis of chagasic cardiomyopathy and provide new targets for intervention.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 423-432 |
| Number of pages | 10 |
| Journal | Genomics |
| Volume | 91 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 2008 |
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Keywords
- Cardiomyopathy
- Chagas disease
- Fas
- Gene array analysis
- Infection/inflammation
- Trypanosoma cruzi; Cyclins
ASJC Scopus subject areas
- Genetics
Cite this
Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection. / Mukherjee, Shankar; Nagajyothi, Fnu; Mukhopadhyay, Aparna; Machado, Fabiana S.; Belbin, Thomas J.; Campos de Carvalho, Antonio; Guan, Fangxia; Albanese, Chris; Jelicks, Linda A.; Lisanti, Michael P.; Silva, Joao S.; Spray, David C.; Weiss, Louis M.; Tanowitz, Herbert B.
In: Genomics, Vol. 91, No. 5, 05.2008, p. 423-432.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection
AU - Mukherjee, Shankar
AU - Nagajyothi, Fnu
AU - Mukhopadhyay, Aparna
AU - Machado, Fabiana S.
AU - Belbin, Thomas J.
AU - Campos de Carvalho, Antonio
AU - Guan, Fangxia
AU - Albanese, Chris
AU - Jelicks, Linda A.
AU - Lisanti, Michael P.
AU - Silva, Joao S.
AU - Spray, David C.
AU - Weiss, Louis M.
AU - Tanowitz, Herbert B.
PY - 2008/5
Y1 - 2008/5
N2 - Chagas disease, characterized by acute myocarditis and chronic cardiomyopathy, is caused by infection with the protozoan parasite Trypanosoma cruzi. We sought to identify genes altered during the development of parasite-induced cardiomyopathy. Microarrays containing 27,400 sequence-verified mouse cDNAs were used to analyze global gene expression changes in the myocardium of a murine model of chagasic cardiomyopathy. Changes in gene expression were determined as the acute stage of infection developed into the chronic stage. This analysis was performed on the hearts of male CD-1 mice infected with trypomastigotes of T. cruzi (Brazil strain). At each interval we compared infected and uninfected mice and confirmed the microarray data with dye reversal. We identified eight distinct categories of mRNAs that were differentially regulated during infection and identified dysregulation of several key genes. These data may provide insight into the pathogenesis of chagasic cardiomyopathy and provide new targets for intervention.
AB - Chagas disease, characterized by acute myocarditis and chronic cardiomyopathy, is caused by infection with the protozoan parasite Trypanosoma cruzi. We sought to identify genes altered during the development of parasite-induced cardiomyopathy. Microarrays containing 27,400 sequence-verified mouse cDNAs were used to analyze global gene expression changes in the myocardium of a murine model of chagasic cardiomyopathy. Changes in gene expression were determined as the acute stage of infection developed into the chronic stage. This analysis was performed on the hearts of male CD-1 mice infected with trypomastigotes of T. cruzi (Brazil strain). At each interval we compared infected and uninfected mice and confirmed the microarray data with dye reversal. We identified eight distinct categories of mRNAs that were differentially regulated during infection and identified dysregulation of several key genes. These data may provide insight into the pathogenesis of chagasic cardiomyopathy and provide new targets for intervention.
KW - Cardiomyopathy
KW - Chagas disease
KW - Fas
KW - Gene array analysis
KW - Infection/inflammation
KW - Trypanosoma cruzi; Cyclins
UR - http://www.scopus.com/inward/record.url?scp=42049104924&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=42049104924&partnerID=8YFLogxK
U2 - 10.1016/j.ygeno.2008.01.008
DO - 10.1016/j.ygeno.2008.01.008
M3 - Article
C2 - 18343633
AN - SCOPUS:42049104924
VL - 91
SP - 423
EP - 432
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 5
ER -