Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection

Shankar Mukherjee, Fnu Nagajyothi, Aparna Mukhopadhyay, Fabiana S. Machado, Thomas J. Belbin, Antonio Campos de Carvalho, Fangxia Guan, Chris Albanese, Linda A. Jelicks, Michael P. Lisanti, Joao S. Silva, David C. Spray, Louis M. Weiss, Herbert B. Tanowitz

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Chagas disease, characterized by acute myocarditis and chronic cardiomyopathy, is caused by infection with the protozoan parasite Trypanosoma cruzi. We sought to identify genes altered during the development of parasite-induced cardiomyopathy. Microarrays containing 27,400 sequence-verified mouse cDNAs were used to analyze global gene expression changes in the myocardium of a murine model of chagasic cardiomyopathy. Changes in gene expression were determined as the acute stage of infection developed into the chronic stage. This analysis was performed on the hearts of male CD-1 mice infected with trypomastigotes of T. cruzi (Brazil strain). At each interval we compared infected and uninfected mice and confirmed the microarray data with dye reversal. We identified eight distinct categories of mRNAs that were differentially regulated during infection and identified dysregulation of several key genes. These data may provide insight into the pathogenesis of chagasic cardiomyopathy and provide new targets for intervention.

Original languageEnglish (US)
Pages (from-to)423-432
Number of pages10
JournalGenomics
Volume91
Issue number5
DOIs
StatePublished - May 1 2008

Keywords

  • Cardiomyopathy
  • Chagas disease
  • Fas
  • Gene array analysis
  • Infection/inflammation
  • Trypanosoma cruzi; Cyclins

ASJC Scopus subject areas

  • Genetics

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    Mukherjee, S., Nagajyothi, F., Mukhopadhyay, A., Machado, F. S., Belbin, T. J., Campos de Carvalho, A., Guan, F., Albanese, C., Jelicks, L. A., Lisanti, M. P., Silva, J. S., Spray, D. C., Weiss, L. M., & Tanowitz, H. B. (2008). Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection. Genomics, 91(5), 423-432. https://doi.org/10.1016/j.ygeno.2008.01.008