Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection

Shankar Mukherjee; Fnu Nagajyothi; Aparna Mukhopadhyay; Fabiana S. Machado; Thomas J. Belbin; Antonio Campos de Carvalho; Fangxia Guan; Chris Albanese; Linda A. Jelicks; Michael P. Lisanti; Joao S. Silva; David C. Spray; Louis M. Weiss; Herbert B. Tanowitz

doi:10.1016/j.ygeno.2008.01.008

Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection

Shankar Mukherjee, Fnu Nagajyothi, Aparna Mukhopadhyay, Fabiana S. Machado, Thomas J. Belbin, Antonio Campos de Carvalho, Fangxia Guan, Chris Albanese, Linda A. Jelicks, Michael P. Lisanti, Joao S. Silva, David C. Spray, Louis M. Weiss, Herbert B. Tanowitz

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Chagas disease, characterized by acute myocarditis and chronic cardiomyopathy, is caused by infection with the protozoan parasite Trypanosoma cruzi. We sought to identify genes altered during the development of parasite-induced cardiomyopathy. Microarrays containing 27,400 sequence-verified mouse cDNAs were used to analyze global gene expression changes in the myocardium of a murine model of chagasic cardiomyopathy. Changes in gene expression were determined as the acute stage of infection developed into the chronic stage. This analysis was performed on the hearts of male CD-1 mice infected with trypomastigotes of T. cruzi (Brazil strain). At each interval we compared infected and uninfected mice and confirmed the microarray data with dye reversal. We identified eight distinct categories of mRNAs that were differentially regulated during infection and identified dysregulation of several key genes. These data may provide insight into the pathogenesis of chagasic cardiomyopathy and provide new targets for intervention.

Original language	English (US)
Pages (from-to)	423-432
Number of pages	10
Journal	Genomics
Volume	91
Issue number	5
DOIs	https://doi.org/10.1016/j.ygeno.2008.01.008
State	Published - May 2008

Keywords

Cardiomyopathy
Chagas disease
Fas
Gene array analysis
Infection/inflammation
Trypanosoma cruzi; Cyclins

ASJC Scopus subject areas

Genetics

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Mukherjee, S., Nagajyothi, F., Mukhopadhyay, A., Machado, F. S., Belbin, T. J., Campos de Carvalho, A., Guan, F., Albanese, C., Jelicks, L. A., Lisanti, M. P., Silva, J. S., Spray, D. C., Weiss, L. M., & Tanowitz, H. B. (2008). Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection. Genomics, 91(5), 423-432. https://doi.org/10.1016/j.ygeno.2008.01.008

Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection. / Mukherjee, Shankar; Nagajyothi, Fnu; Mukhopadhyay, Aparna; Machado, Fabiana S.; Belbin, Thomas J.; Campos de Carvalho, Antonio; Guan, Fangxia; Albanese, Chris; Jelicks, Linda A.; Lisanti, Michael P.; Silva, Joao S.; Spray, David C.; Weiss, Louis M.; Tanowitz, Herbert B.

In: Genomics, Vol. 91, No. 5, 05.2008, p. 423-432.

Research output: Contribution to journal › Article › peer-review

Mukherjee, S, Nagajyothi, F, Mukhopadhyay, A, Machado, FS, Belbin, TJ, Campos de Carvalho, A, Guan, F, Albanese, C, Jelicks, LA, Lisanti, MP, Silva, JS, Spray, DC , Weiss, LM & Tanowitz, HB 2008, 'Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection', Genomics, vol. 91, no. 5, pp. 423-432. https://doi.org/10.1016/j.ygeno.2008.01.008

Mukherjee, Shankar ; Nagajyothi, Fnu ; Mukhopadhyay, Aparna ; Machado, Fabiana S. ; Belbin, Thomas J. ; Campos de Carvalho, Antonio ; Guan, Fangxia ; Albanese, Chris ; Jelicks, Linda A. ; Lisanti, Michael P. ; Silva, Joao S. ; Spray, David C. ; Weiss, Louis M. ; Tanowitz, Herbert B. / Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection. In: Genomics. 2008 ; Vol. 91, No. 5. pp. 423-432.

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title = "Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection",

abstract = "Chagas disease, characterized by acute myocarditis and chronic cardiomyopathy, is caused by infection with the protozoan parasite Trypanosoma cruzi. We sought to identify genes altered during the development of parasite-induced cardiomyopathy. Microarrays containing 27,400 sequence-verified mouse cDNAs were used to analyze global gene expression changes in the myocardium of a murine model of chagasic cardiomyopathy. Changes in gene expression were determined as the acute stage of infection developed into the chronic stage. This analysis was performed on the hearts of male CD-1 mice infected with trypomastigotes of T. cruzi (Brazil strain). At each interval we compared infected and uninfected mice and confirmed the microarray data with dye reversal. We identified eight distinct categories of mRNAs that were differentially regulated during infection and identified dysregulation of several key genes. These data may provide insight into the pathogenesis of chagasic cardiomyopathy and provide new targets for intervention.",

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note = "Funding Information: This work was supported by AHA SDG 0735252N (S.M.) and NIH Grants AI-052739 and AI-068538 (H.B.T.), HL-073732 (A.C.), and AI022730 (L.A.J.). ",

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AU - Silva, Joao S.

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AU - Weiss, Louis M.

AU - Tanowitz, Herbert B.

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