Alteration of Intramolecular Disulfides in Insulin Receptor/Kinase by Insulin and Dithiothreitol

Insulin Potentiates the Apparent Dithiothreitol-Dependent Subunit Reduction of Insulin Receptor

Peter A. Wilden, Timothy R. Boyle, Michael L. Swanson, Laurel J. Sweet, Jeffrey E. Pessin

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Dithiothreitol (DTT) was observed to increase both β-subunit autophosphorylation and exogenous substrate phosphorylation of the insulin receptor in the absence of insulin. The natural protein reducing agent thioredoxin was also observed to increase the insulin receptor β-subunit autophosphorylation. The activation of the insulin receptor/kinase by both DTT and thioredoxin was found to be additive with that of insulin. Further, the increase in the insulin receptor β-subunit autophosphorylation in the presence of DTT and insulin was demonstrated to be due to an increase in the initial rate of autophosphorylation without alteration in the extent of phosphorylation. Similarly, the increase in the exogenous substrate phosphorylation was due to an increase in the Vmax of phosphorylation without significant effect on the apparent Km of substrate binding. In the presence of relatively low concentrations of DTT, insulin was found to potentiate the apparent insulin receptor subunit reduction of the native α2β2 heterotetrameric complex into αβ heterodimers, when observed by silver staining of sodium dodecyl sulfate-polyacrylamide gels.-[3H]Ethylmaleimide ([3H]NEM) labeling in the absence of DTT pretreatment demonstrated that only the β subunit had accessible sulfhydryl group(s). However, treatment of insulin receptors with DTT increased the amount of [3H]NEM labeling in the β subunit as well as exposing sites on the α subunit. Further, incubation of the insulin receptors with the combination of DTT and insulin also demonstrated the apparent insulin-potentiated subunit reduction without any increase in the total amount of [3H]NEM labeling. These results suggest that the insulin activation of the insulin receptor/kinase involves an increased sensitivity of the insulin receptor to reducing agents without any alteration in the total number of accessible sulfhydryl groups.

Original languageEnglish (US)
Pages (from-to)4381-4388
Number of pages8
JournalBiochemistry
Volume25
Issue number15
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

Dithiothreitol
Insulin Receptor
Disulfides
Phosphotransferases
Insulin
Phosphorylation
Labeling
Thioredoxins
Reducing Agents
Substrates
Chemical activation
Silver Staining
Ethylmaleimide
Silver
Sodium Dodecyl Sulfate

ASJC Scopus subject areas

  • Biochemistry

Cite this

Alteration of Intramolecular Disulfides in Insulin Receptor/Kinase by Insulin and Dithiothreitol : Insulin Potentiates the Apparent Dithiothreitol-Dependent Subunit Reduction of Insulin Receptor. / Wilden, Peter A.; Boyle, Timothy R.; Swanson, Michael L.; Sweet, Laurel J.; Pessin, Jeffrey E.

In: Biochemistry, Vol. 25, No. 15, 1986, p. 4381-4388.

Research output: Contribution to journalArticle

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abstract = "Dithiothreitol (DTT) was observed to increase both β-subunit autophosphorylation and exogenous substrate phosphorylation of the insulin receptor in the absence of insulin. The natural protein reducing agent thioredoxin was also observed to increase the insulin receptor β-subunit autophosphorylation. The activation of the insulin receptor/kinase by both DTT and thioredoxin was found to be additive with that of insulin. Further, the increase in the insulin receptor β-subunit autophosphorylation in the presence of DTT and insulin was demonstrated to be due to an increase in the initial rate of autophosphorylation without alteration in the extent of phosphorylation. Similarly, the increase in the exogenous substrate phosphorylation was due to an increase in the Vmax of phosphorylation without significant effect on the apparent Km of substrate binding. In the presence of relatively low concentrations of DTT, insulin was found to potentiate the apparent insulin receptor subunit reduction of the native α2β2 heterotetrameric complex into αβ heterodimers, when observed by silver staining of sodium dodecyl sulfate-polyacrylamide gels.-[3H]Ethylmaleimide ([3H]NEM) labeling in the absence of DTT pretreatment demonstrated that only the β subunit had accessible sulfhydryl group(s). However, treatment of insulin receptors with DTT increased the amount of [3H]NEM labeling in the β subunit as well as exposing sites on the α subunit. Further, incubation of the insulin receptors with the combination of DTT and insulin also demonstrated the apparent insulin-potentiated subunit reduction without any increase in the total amount of [3H]NEM labeling. These results suggest that the insulin activation of the insulin receptor/kinase involves an increased sensitivity of the insulin receptor to reducing agents without any alteration in the total number of accessible sulfhydryl groups.",
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AU - Pessin, Jeffrey E.

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N2 - Dithiothreitol (DTT) was observed to increase both β-subunit autophosphorylation and exogenous substrate phosphorylation of the insulin receptor in the absence of insulin. The natural protein reducing agent thioredoxin was also observed to increase the insulin receptor β-subunit autophosphorylation. The activation of the insulin receptor/kinase by both DTT and thioredoxin was found to be additive with that of insulin. Further, the increase in the insulin receptor β-subunit autophosphorylation in the presence of DTT and insulin was demonstrated to be due to an increase in the initial rate of autophosphorylation without alteration in the extent of phosphorylation. Similarly, the increase in the exogenous substrate phosphorylation was due to an increase in the Vmax of phosphorylation without significant effect on the apparent Km of substrate binding. In the presence of relatively low concentrations of DTT, insulin was found to potentiate the apparent insulin receptor subunit reduction of the native α2β2 heterotetrameric complex into αβ heterodimers, when observed by silver staining of sodium dodecyl sulfate-polyacrylamide gels.-[3H]Ethylmaleimide ([3H]NEM) labeling in the absence of DTT pretreatment demonstrated that only the β subunit had accessible sulfhydryl group(s). However, treatment of insulin receptors with DTT increased the amount of [3H]NEM labeling in the β subunit as well as exposing sites on the α subunit. Further, incubation of the insulin receptors with the combination of DTT and insulin also demonstrated the apparent insulin-potentiated subunit reduction without any increase in the total amount of [3H]NEM labeling. These results suggest that the insulin activation of the insulin receptor/kinase involves an increased sensitivity of the insulin receptor to reducing agents without any alteration in the total number of accessible sulfhydryl groups.

AB - Dithiothreitol (DTT) was observed to increase both β-subunit autophosphorylation and exogenous substrate phosphorylation of the insulin receptor in the absence of insulin. The natural protein reducing agent thioredoxin was also observed to increase the insulin receptor β-subunit autophosphorylation. The activation of the insulin receptor/kinase by both DTT and thioredoxin was found to be additive with that of insulin. Further, the increase in the insulin receptor β-subunit autophosphorylation in the presence of DTT and insulin was demonstrated to be due to an increase in the initial rate of autophosphorylation without alteration in the extent of phosphorylation. Similarly, the increase in the exogenous substrate phosphorylation was due to an increase in the Vmax of phosphorylation without significant effect on the apparent Km of substrate binding. In the presence of relatively low concentrations of DTT, insulin was found to potentiate the apparent insulin receptor subunit reduction of the native α2β2 heterotetrameric complex into αβ heterodimers, when observed by silver staining of sodium dodecyl sulfate-polyacrylamide gels.-[3H]Ethylmaleimide ([3H]NEM) labeling in the absence of DTT pretreatment demonstrated that only the β subunit had accessible sulfhydryl group(s). However, treatment of insulin receptors with DTT increased the amount of [3H]NEM labeling in the β subunit as well as exposing sites on the α subunit. Further, incubation of the insulin receptors with the combination of DTT and insulin also demonstrated the apparent insulin-potentiated subunit reduction without any increase in the total amount of [3H]NEM labeling. These results suggest that the insulin activation of the insulin receptor/kinase involves an increased sensitivity of the insulin receptor to reducing agents without any alteration in the total number of accessible sulfhydryl groups.

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