TY - JOUR
T1 - Alteration of bile acid metabolism in the rat induced by chronic ethanol consumption
AU - Xie, Guoxiang
AU - Zhong, Wei
AU - Li, Houkai
AU - Li, Qiong
AU - Qiu, Yunping
AU - Zheng, Xiaojiao
AU - Chen, Huiyuan
AU - Zhao, Xueqing
AU - Zhang, Shucha
AU - Zhou, Zhanxiang
AU - Zeisel, Steven H.
AU - Jia, Wei
PY - 2013/9
Y1 - 2013/9
N2 - Our understanding of the bile acid metabolism is limited by the fact that previous analyses have primarily focused on a selected few circulating bile acids; the bile acid profiles of the liver and gastrointestinal tract pools are rarely investigated. Here, we determined how chronic ethanol consumption altered the bile acids in multiple body compartments (liver, gastrointestinal tract, and serum) of rats. Rats were fed a modified Lieber-DeCarli liquid diet with 38% of calories as ethanol (the amount equivalent of 4-5 drinks in humans). While conjugated bile acids predominated in the liver (98.3%), duodenum (97.8%), and ileum (89.7%), unconjugated bile acids comprised the largest proportion of measured bile acids in serum (81.2%), the cecum (97.7%), and the rectum (97.5%). In particular, taurine-conjugated bile acids were significantly decreased in the liver and gastrointestinal tract of ethanol-treated rats, while unconjugated and glycineconjugated species increased. Ethanol consumption caused increased expression of genes involved in bile acid biosynthesis, efflux transport, and reduced expression of genes regulating bile acid influx transport in the liver. These results provide an improved understanding of the systemic modulations of bile acid metabolism in mammals through the gut-liver axis.-Xie, G., Zhong, W., Li, H., Li, Q., Qiu, Y., Zheng, X., Chen, H., Zhao, X., Zhang, S., Zhou, Z., Zeisel, S. H., Jia, W. Alteration of bile acid metabolism in the rat induced by chronic ethanol consumption.
AB - Our understanding of the bile acid metabolism is limited by the fact that previous analyses have primarily focused on a selected few circulating bile acids; the bile acid profiles of the liver and gastrointestinal tract pools are rarely investigated. Here, we determined how chronic ethanol consumption altered the bile acids in multiple body compartments (liver, gastrointestinal tract, and serum) of rats. Rats were fed a modified Lieber-DeCarli liquid diet with 38% of calories as ethanol (the amount equivalent of 4-5 drinks in humans). While conjugated bile acids predominated in the liver (98.3%), duodenum (97.8%), and ileum (89.7%), unconjugated bile acids comprised the largest proportion of measured bile acids in serum (81.2%), the cecum (97.7%), and the rectum (97.5%). In particular, taurine-conjugated bile acids were significantly decreased in the liver and gastrointestinal tract of ethanol-treated rats, while unconjugated and glycineconjugated species increased. Ethanol consumption caused increased expression of genes involved in bile acid biosynthesis, efflux transport, and reduced expression of genes regulating bile acid influx transport in the liver. These results provide an improved understanding of the systemic modulations of bile acid metabolism in mammals through the gut-liver axis.-Xie, G., Zhong, W., Li, H., Li, Q., Qiu, Y., Zheng, X., Chen, H., Zhao, X., Zhang, S., Zhou, Z., Zeisel, S. H., Jia, W. Alteration of bile acid metabolism in the rat induced by chronic ethanol consumption.
KW - Blood
KW - Gastrointestinal tract
KW - Liver
KW - Metabolomics
KW - Ultraperformance liquid chromatography mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=84883395327&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883395327&partnerID=8YFLogxK
U2 - 10.1096/fj.13-231860
DO - 10.1096/fj.13-231860
M3 - Article
C2 - 23709616
AN - SCOPUS:84883395327
SN - 0892-6638
VL - 27
SP - 3583
EP - 3593
JO - FASEB Journal
JF - FASEB Journal
IS - 9
ER -