alpha-Bungarotoxin labeling and acetylcholinesterase localization at the Mauthner fiber giant synapse in the hatchetfish.

J. W. Day, D. H. Hall, L. M. Hall, M. V. Bennett

Research output: Contribution to journalArticle

Abstract

Autoradiographic and histochemical techniques have been used to characterize further the pharmacology of transmission at the Mauthner fiber giant synapse of the South American hatchetfish. [125I]alpha-Bungarotoxin was applied to hatchetfish medullae and a standard autoradiographic procedure was carried out on 3- to 4-microns sections of glutaraldehyde-fixed tissue. All Mauthner fiber giant synapses, as identified by light microscopic criteria, had closely associated silver grains. Labeling was blocked by d-tubocurarine. Glutaraldehyde-fixed slices of hatchetfish medulla were stained histochemically for acetylcholinesterase; all giant synapses that could be identified in the light microscope showed heavy deposits of reaction product. Staining was blocked by diisopropyl-fluorophosphate, which inhibits both pseudocholinesterase and acetylcholinesterase, but was not blocked by tetraisopropylpyrophosphoramide, a specific pseudocholinesterase inhibitor. This evidence strongly supports the suggestion that the Mauthner fiber giant synapse is nicotinic cholinergic.

Original languageEnglish (US)
Pages (from-to)272-279
Number of pages8
JournalJournal of Neuroscience
Volume3
Issue number2
StatePublished - Feb 1983
Externally publishedYes

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Bungarotoxins
Acetylcholinesterase
Synapses
Pseudocholinesterase
fluorophosphate
Glutaral
Light
Tubocurarine
Silver
Cholinergic Agents
Pharmacology
Staining and Labeling

ASJC Scopus subject areas

  • Neuroscience(all)

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alpha-Bungarotoxin labeling and acetylcholinesterase localization at the Mauthner fiber giant synapse in the hatchetfish. / Day, J. W.; Hall, D. H.; Hall, L. M.; Bennett, M. V.

In: Journal of Neuroscience, Vol. 3, No. 2, 02.1983, p. 272-279.

Research output: Contribution to journalArticle

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