Alkaline phosphatase band-10 fraction as a possible surrogate marker for human immunodeficiency virus type 1 infection in children

V. V. Murthy, T. A. Calvelli, Arye Rubinstein, A. Karmen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We report the utility of a possible lymphocyte fraction of alkaline phosphatase (ALP band-10) activity in serum to predict human immunodeficiency virus type 1 (HIV-1) infection in children born to HIV-1-seropositive mothers. The presence of ALP band 10 in serum consistently correlated with HIV-1 infection status as judged by positive HIV-1 culture, two consecutive HIV-1 p24 antigen results greater than 30 pg/mL in serum, and the subsequent confirmation of seroconversion to HIV-1 antibody after clearance of maternal IgG anti-HIV-1 antibody ascertained between 15 to 24 months post partum. Infection with HIV-1 was correctly identified in 31 samples from 18 patients ranging in age between 0.1 to 10 years; the absence of similar infection was noted in 14 samples from nine patients who served as controls and whose serum samples did not exhibit ALP band-10 activity. This ability of serum ALP band- 10 activity to predict HIV-1 infection status in children as young as 2 months may be useful as a surrogate marker for early identification of HIV-1 infection in infants born to HIV-1-seropositive women long before the clearance of maternal anti-HIV-1 antibodies can be ascertained.

Original languageEnglish (US)
Pages (from-to)873-877
Number of pages5
JournalArchives of Pathology and Laboratory Medicine
Volume118
Issue number9
StatePublished - 1994

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Virus Diseases
Alkaline Phosphatase
HIV-1
Biomarkers
Serum
Mothers
Antibodies
Aptitude
Infection
Lymphocytes
Antigens

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

Alkaline phosphatase band-10 fraction as a possible surrogate marker for human immunodeficiency virus type 1 infection in children. / Murthy, V. V.; Calvelli, T. A.; Rubinstein, Arye; Karmen, A.

In: Archives of Pathology and Laboratory Medicine, Vol. 118, No. 9, 1994, p. 873-877.

Research output: Contribution to journalArticle

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