Alkaline phosphatase activity in cultured meningioma cells

N. Takahara, F. Herz, A. Hirano

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The specific activity of alkaline phosphatase in cultured human meningioma cells varies over a relatively wide range. There is no correlation between the levels of activity and the histological type of meningioma from which the cultures were derived. The enzyme is heat-labile and is strongly inhibited by l-homoarginine, levamisole, and l-bromotetramisole, but unaffected by l-phenylalanine and l-phenylalanylglycylglycine. These findings indicate that meningioma cells synthesize the liver/bone/kidney form of alkaline phosphatase. In contrast to cultures derived from pituitary adenomas, glioblastomas, and astrocytomas in which prednisolone and/or sodium butyrate elicit a manifold increase of alkaline phosphatase activity, with meningioma cells the hormone causes only a slight augmentation in specific activity, and the fatty acid is ineffective. As with other cells producing the liver/bone/kidney enzyme form, no increase in activity occurs in meningioma cells growing in hyperosmolar medium.

Original languageEnglish (US)
Pages (from-to)45-50
Number of pages6
JournalActa Neuropathologica
Volume57
Issue number1
DOIs
StatePublished - Mar 1982

Fingerprint

Meningioma
Alkaline Phosphatase
Cultured Cells
Homoarginine
Kidney
Bone and Bones
Levamisole
Butyric Acid
Liver
Astrocytoma
Pituitary Neoplasms
Enzymes
Glioblastoma
Prednisolone
Phenylalanine
Fatty Acids
Hot Temperature
Hormones

Keywords

  • Alkaline phosphatase
  • Cell culture
  • Meningioma

ASJC Scopus subject areas

  • Neuroscience(all)
  • Pathology and Forensic Medicine
  • Clinical Neurology

Cite this

Alkaline phosphatase activity in cultured meningioma cells. / Takahara, N.; Herz, F.; Hirano, A.

In: Acta Neuropathologica, Vol. 57, No. 1, 03.1982, p. 45-50.

Research output: Contribution to journalArticle

Takahara, N. ; Herz, F. ; Hirano, A. / Alkaline phosphatase activity in cultured meningioma cells. In: Acta Neuropathologica. 1982 ; Vol. 57, No. 1. pp. 45-50.
@article{00a53f94174c4f96a587bfc73b16df43,
title = "Alkaline phosphatase activity in cultured meningioma cells",
abstract = "The specific activity of alkaline phosphatase in cultured human meningioma cells varies over a relatively wide range. There is no correlation between the levels of activity and the histological type of meningioma from which the cultures were derived. The enzyme is heat-labile and is strongly inhibited by l-homoarginine, levamisole, and l-bromotetramisole, but unaffected by l-phenylalanine and l-phenylalanylglycylglycine. These findings indicate that meningioma cells synthesize the liver/bone/kidney form of alkaline phosphatase. In contrast to cultures derived from pituitary adenomas, glioblastomas, and astrocytomas in which prednisolone and/or sodium butyrate elicit a manifold increase of alkaline phosphatase activity, with meningioma cells the hormone causes only a slight augmentation in specific activity, and the fatty acid is ineffective. As with other cells producing the liver/bone/kidney enzyme form, no increase in activity occurs in meningioma cells growing in hyperosmolar medium.",
keywords = "Alkaline phosphatase, Cell culture, Meningioma",
author = "N. Takahara and F. Herz and A. Hirano",
year = "1982",
month = "3",
doi = "10.1007/BF00688876",
language = "English (US)",
volume = "57",
pages = "45--50",
journal = "Acta Neuropathologica",
issn = "0001-6322",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Alkaline phosphatase activity in cultured meningioma cells

AU - Takahara, N.

AU - Herz, F.

AU - Hirano, A.

PY - 1982/3

Y1 - 1982/3

N2 - The specific activity of alkaline phosphatase in cultured human meningioma cells varies over a relatively wide range. There is no correlation between the levels of activity and the histological type of meningioma from which the cultures were derived. The enzyme is heat-labile and is strongly inhibited by l-homoarginine, levamisole, and l-bromotetramisole, but unaffected by l-phenylalanine and l-phenylalanylglycylglycine. These findings indicate that meningioma cells synthesize the liver/bone/kidney form of alkaline phosphatase. In contrast to cultures derived from pituitary adenomas, glioblastomas, and astrocytomas in which prednisolone and/or sodium butyrate elicit a manifold increase of alkaline phosphatase activity, with meningioma cells the hormone causes only a slight augmentation in specific activity, and the fatty acid is ineffective. As with other cells producing the liver/bone/kidney enzyme form, no increase in activity occurs in meningioma cells growing in hyperosmolar medium.

AB - The specific activity of alkaline phosphatase in cultured human meningioma cells varies over a relatively wide range. There is no correlation between the levels of activity and the histological type of meningioma from which the cultures were derived. The enzyme is heat-labile and is strongly inhibited by l-homoarginine, levamisole, and l-bromotetramisole, but unaffected by l-phenylalanine and l-phenylalanylglycylglycine. These findings indicate that meningioma cells synthesize the liver/bone/kidney form of alkaline phosphatase. In contrast to cultures derived from pituitary adenomas, glioblastomas, and astrocytomas in which prednisolone and/or sodium butyrate elicit a manifold increase of alkaline phosphatase activity, with meningioma cells the hormone causes only a slight augmentation in specific activity, and the fatty acid is ineffective. As with other cells producing the liver/bone/kidney enzyme form, no increase in activity occurs in meningioma cells growing in hyperosmolar medium.

KW - Alkaline phosphatase

KW - Cell culture

KW - Meningioma

UR - http://www.scopus.com/inward/record.url?scp=0020076903&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020076903&partnerID=8YFLogxK

U2 - 10.1007/BF00688876

DO - 10.1007/BF00688876

M3 - Article

C2 - 7090741

AN - SCOPUS:0020076903

VL - 57

SP - 45

EP - 50

JO - Acta Neuropathologica

JF - Acta Neuropathologica

SN - 0001-6322

IS - 1

ER -