Alkaline phosphatase activity in cultured meningioma cells

N. Takahara, F. Herz, A. Hirano

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The specific activity of alkaline phosphatase in cultured human meningioma cells varies over a relatively wide range. There is no correlation between the levels of activity and the histological type of meningioma from which the cultures were derived. The enzyme is heat-labile and is strongly inhibited by l-homoarginine, levamisole, and l-bromotetramisole, but unaffected by l-phenylalanine and l-phenylalanylglycylglycine. These findings indicate that meningioma cells synthesize the liver/bone/kidney form of alkaline phosphatase. In contrast to cultures derived from pituitary adenomas, glioblastomas, and astrocytomas in which prednisolone and/or sodium butyrate elicit a manifold increase of alkaline phosphatase activity, with meningioma cells the hormone causes only a slight augmentation in specific activity, and the fatty acid is ineffective. As with other cells producing the liver/bone/kidney enzyme form, no increase in activity occurs in meningioma cells growing in hyperosmolar medium.

Original languageEnglish (US)
Pages (from-to)45-50
Number of pages6
JournalActa neuropathologica
Volume57
Issue number1
DOIs
Publication statusPublished - Mar 1 1982

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Keywords

  • Alkaline phosphatase
  • Cell culture
  • Meningioma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

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