Aldolase mediates the association of F-actin with the insulin-responsive glucose transporter GLUT4

Aimee W. Kao, Yoichi Noda, John H. Johnson, Jeffrey E. Pessin, Alan R. Saltiel

Research output: Contribution to journalArticle

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Abstract

To identify potential proteins interacting with the insulin-responsive glucose transporter (GLUT4), we generated fusion proteins of glutathione S- transferase (GST) and the final 30 amino acids from GLUT4 (GST-G4) or GLUT1 (GST-G1). Incubation of these carboxylterminal fusion proteins with adipocyte cell extracts revealed a specific interaction of GLUT4 with fructose 1,6- bisphosphate aldolase. In the presence of aldolase, GST-G4 but not GST-G1 was able to co-pellet with filamentous (F)-actin. This interaction was prevented by incubation with the aldolase substrates, fructose 1,6-bisphosphate or glyceraldehyde 3-phosphate. Immunofluorescence confocal microscopy demonstrated a significant co-localization of aldolase and GLUT4 in intact 3T3L1 adipocytes, which decreased following insulin stimulation. Introduction into permeabilized 3T3L1 adipocytes of fructose 1,6-bisphosphate or the metabolic inhibitor 2-deoxyglucose, two agents that disrupt the interaction between aldolase and actin, inhibited insulin-stimulated GLUT4 exocytosis without affecting GLUT4 endocytosis. Furthermore, microinjection of an aldolase-specific antibody also inhibited insulin-stimulated GLUT4 translocation. These data suggest that aldolase functions as a scaffolding protein for GLUT4 and that glucose metabolism may provide a negative feedback signal for the regulation of glucose transport by insulin.

Original languageEnglish (US)
Pages (from-to)17742-17747
Number of pages6
JournalJournal of Biological Chemistry
Volume274
Issue number25
DOIs
StatePublished - Jun 18 1999
Externally publishedYes

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Glucose Transporter Type 4
Fructose-Bisphosphate Aldolase
Actins
Glutathione Transferase
Adipocytes
Insulin
Glyceraldehyde 3-Phosphate
Fusion reactions
Glucose
Protein S
Exocytosis
Deoxyglucose
Microinjections
Proteins
Endocytosis
Cell Extracts
Confocal microscopy
Fluorescence Microscopy
Confocal Microscopy
Metabolism

ASJC Scopus subject areas

  • Biochemistry

Cite this

Aldolase mediates the association of F-actin with the insulin-responsive glucose transporter GLUT4. / Kao, Aimee W.; Noda, Yoichi; Johnson, John H.; Pessin, Jeffrey E.; Saltiel, Alan R.

In: Journal of Biological Chemistry, Vol. 274, No. 25, 18.06.1999, p. 17742-17747.

Research output: Contribution to journalArticle

Kao, Aimee W. ; Noda, Yoichi ; Johnson, John H. ; Pessin, Jeffrey E. ; Saltiel, Alan R. / Aldolase mediates the association of F-actin with the insulin-responsive glucose transporter GLUT4. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 25. pp. 17742-17747.
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