Airway microbiota is associated with upregulation of the PI3K pathway in lung cancer

Jun Chieh J. Tsay, Benjamin G. Wu, Michelle H. Badri, Jose C. Clemente, Nan Shen, Peter Meyn, Yonghua Li, Ting An Yie, Tenzin Lhakhang, Evan Olsen, Vivek Murthy, Gaetane Michaud, Imran Sulaiman, Aristotelis Tsirigos, Adriana Heguy, Harvey Pass, Michael D. Weiden, William N. Rom, Daniel H. Sterman, Richard BonneauMartin J. Blaser, Leopoldo N. Segal

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Rationale: Inlung cancer,upregulationof the PI3K(phosphoinositide 3-kinase) pathway is an early event that contributes to cell proliferation, survival, and tissue invasion. Upregulation of this pathway was recently described as associated with enrichment of the lower airways with bacteria identified as oral commensals. Objectives: We hypothesize that host-microbe interactions in the lower airways of subjects with lung cancer affect known cancer pathways. Methods: Airway brushings were collected prospectively from subjects with lung nodules at time of diagnostic bronchoscopy, including 39 subjects with final lung cancer diagnoses and 36 subjects with noncancer diagnoses. In addition, samples from 10 healthy control subjects were included. 16S ribosomal RNA gene amplicon sequencing and paired transcriptome sequencing were performed on all airway samples. In addition, an in vitro model with airway epithelial cells exposed to bacteria/bacterial products was performed. Measurements and Main Results: The composition of the lower airway transcriptome in the patients with cancer was significantly different from the control subjects, which included up-regulation of ERK (extracellular signal-regulated kinase) and PI3K signaling pathways. The lower airways of patients with lung cancer were enriched for oral taxa (Streptococcus and Veillonella), which was associated with up-regulation of the ERK and PI3K signaling pathways. In vitro exposure of airway epithelial cells to Veillonella, Prevotella, and Streptococcus led to upregulation of these same signaling pathways. Conclusions: The data presented here show that several transcriptomic signatures previously identified as relevant to lung cancer pathogenesis are associated with enrichment of the lower airway microbiota with oral commensals.

Original languageEnglish (US)
Pages (from-to)1188-1198
Number of pages11
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume198
Issue number9
DOIs
StatePublished - Nov 1 2018
Externally publishedYes

Fingerprint

1-Phosphatidylinositol 4-Kinase
Microbiota
Lung Neoplasms
Mitogen-Activated Protein Kinase 6
Up-Regulation
Veillonella
Streptococcus
Transcriptome
Epithelial Cells
Prevotella
16S Ribosomal RNA
Bacteria
Neoplasms
Bronchoscopy
rRNA Genes
Cell Survival
Healthy Volunteers
Cell Proliferation
Lung
In Vitro Techniques

Keywords

  • bronchoscopy
  • lung cancer
  • microbiome

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Tsay, J. C. J., Wu, B. G., Badri, M. H., Clemente, J. C., Shen, N., Meyn, P., ... Segal, L. N. (2018). Airway microbiota is associated with upregulation of the PI3K pathway in lung cancer. American Journal of Respiratory and Critical Care Medicine, 198(9), 1188-1198. https://doi.org/10.1164/rccm.201710-2118OC

Airway microbiota is associated with upregulation of the PI3K pathway in lung cancer. / Tsay, Jun Chieh J.; Wu, Benjamin G.; Badri, Michelle H.; Clemente, Jose C.; Shen, Nan; Meyn, Peter; Li, Yonghua; Yie, Ting An; Lhakhang, Tenzin; Olsen, Evan; Murthy, Vivek; Michaud, Gaetane; Sulaiman, Imran; Tsirigos, Aristotelis; Heguy, Adriana; Pass, Harvey; Weiden, Michael D.; Rom, William N.; Sterman, Daniel H.; Bonneau, Richard; Blaser, Martin J.; Segal, Leopoldo N.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 198, No. 9, 01.11.2018, p. 1188-1198.

Research output: Contribution to journalArticle

Tsay, JCJ, Wu, BG, Badri, MH, Clemente, JC, Shen, N, Meyn, P, Li, Y, Yie, TA, Lhakhang, T, Olsen, E, Murthy, V, Michaud, G, Sulaiman, I, Tsirigos, A, Heguy, A, Pass, H, Weiden, MD, Rom, WN, Sterman, DH, Bonneau, R, Blaser, MJ & Segal, LN 2018, 'Airway microbiota is associated with upregulation of the PI3K pathway in lung cancer', American Journal of Respiratory and Critical Care Medicine, vol. 198, no. 9, pp. 1188-1198. https://doi.org/10.1164/rccm.201710-2118OC
Tsay, Jun Chieh J. ; Wu, Benjamin G. ; Badri, Michelle H. ; Clemente, Jose C. ; Shen, Nan ; Meyn, Peter ; Li, Yonghua ; Yie, Ting An ; Lhakhang, Tenzin ; Olsen, Evan ; Murthy, Vivek ; Michaud, Gaetane ; Sulaiman, Imran ; Tsirigos, Aristotelis ; Heguy, Adriana ; Pass, Harvey ; Weiden, Michael D. ; Rom, William N. ; Sterman, Daniel H. ; Bonneau, Richard ; Blaser, Martin J. ; Segal, Leopoldo N. / Airway microbiota is associated with upregulation of the PI3K pathway in lung cancer. In: American Journal of Respiratory and Critical Care Medicine. 2018 ; Vol. 198, No. 9. pp. 1188-1198.
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T1 - Airway microbiota is associated with upregulation of the PI3K pathway in lung cancer

AU - Tsay, Jun Chieh J.

AU - Wu, Benjamin G.

AU - Badri, Michelle H.

AU - Clemente, Jose C.

AU - Shen, Nan

AU - Meyn, Peter

AU - Li, Yonghua

AU - Yie, Ting An

AU - Lhakhang, Tenzin

AU - Olsen, Evan

AU - Murthy, Vivek

AU - Michaud, Gaetane

AU - Sulaiman, Imran

AU - Tsirigos, Aristotelis

AU - Heguy, Adriana

AU - Pass, Harvey

AU - Weiden, Michael D.

AU - Rom, William N.

AU - Sterman, Daniel H.

AU - Bonneau, Richard

AU - Blaser, Martin J.

AU - Segal, Leopoldo N.

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N2 - Rationale: Inlung cancer,upregulationof the PI3K(phosphoinositide 3-kinase) pathway is an early event that contributes to cell proliferation, survival, and tissue invasion. Upregulation of this pathway was recently described as associated with enrichment of the lower airways with bacteria identified as oral commensals. Objectives: We hypothesize that host-microbe interactions in the lower airways of subjects with lung cancer affect known cancer pathways. Methods: Airway brushings were collected prospectively from subjects with lung nodules at time of diagnostic bronchoscopy, including 39 subjects with final lung cancer diagnoses and 36 subjects with noncancer diagnoses. In addition, samples from 10 healthy control subjects were included. 16S ribosomal RNA gene amplicon sequencing and paired transcriptome sequencing were performed on all airway samples. In addition, an in vitro model with airway epithelial cells exposed to bacteria/bacterial products was performed. Measurements and Main Results: The composition of the lower airway transcriptome in the patients with cancer was significantly different from the control subjects, which included up-regulation of ERK (extracellular signal-regulated kinase) and PI3K signaling pathways. The lower airways of patients with lung cancer were enriched for oral taxa (Streptococcus and Veillonella), which was associated with up-regulation of the ERK and PI3K signaling pathways. In vitro exposure of airway epithelial cells to Veillonella, Prevotella, and Streptococcus led to upregulation of these same signaling pathways. Conclusions: The data presented here show that several transcriptomic signatures previously identified as relevant to lung cancer pathogenesis are associated with enrichment of the lower airway microbiota with oral commensals.

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