AID expression increased by TNF-α is associated with class switch recombination of Igα gene in cancers

Zhi Duan, Hui Zheng, Haidan Liu, Ming Li, Min Tang, Xinxian Weng, Wei Yi, Ann M. Bode, Ya Cao

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Recently, immunoglobulins (Igs) were unexpectedly found to be expressed in epithelial cancers. Immunoglobulin class switching or class switch recombination (CSR) is a natural biological process that alters a B cell's production of antibodies (immunoglobulins) from one class to another. However, the mechanism of CSR of Ig genes in cancer is still unknown. Here, we confirmed by detecting the hallmark of CSR that the Igα gene in cancer underwent CSR. Then we focused on activation-induced cytidine deaminase (AID), a crucial factor for initiating CSR. Further studies using tumor necrosis factor (TNF)-α stimulation and specific inhibitor of NF-κB revealed that TNF-α could increase AID expression through NF-κB signaling. Finally, we demonstrated that AID could co-localize with protein kinase A and bind to the switching (Sα) region of the Igα gene. Overexpression of AID obviously enhanced Igα heavy chain expression and its binding ability to the Sα region. These findings indicated that TNF-α-induced AID expression is involved with CSR in cancer.

Original languageEnglish (US)
Pages (from-to)484-491
Number of pages8
JournalCellular and Molecular Immunology
Volume13
Issue number4
DOIs
StatePublished - Jul 1 2016

Keywords

  • AID
  • Immunoglobulin
  • TNF-α
  • class switch recombination

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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