Agreement for HPV genotyping detection between self-collected specimens on a FTA cartridge and clinician-collected specimens

YaoYao Guan, Patti E. Gravitt, Roslyn Howard, Yolanda J. Eby, Shaoming Wang, Belinda Li, Changyan Feng, You Lin Qiao, Philip E. Castle

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The current method of transporting self-collected cervicovaginal specimen for HPV DNA testing relies on liquid based medium, which is challenging and expensive to transport. A novel, dry storage and transportation device, Whatman indicating FTA™ Elute Cartridge, avoids some of the pitfalls of liquid-based medium. This method has been shown to be comparable to liquid-based collection medium, but relative performance of self-collected (SC) and clinician-collected (CC) samples onto FTA cards has not been reported. The objective of this study is to compare the analytic performance of self- and clinician-collected samples onto FTA cartridges for the detection of carcinogenic HPV using Linear Array. There was a 91% agreement, 69% positive agreement, and kappa of 0.75 between the clinician-collected and self-collected specimens for detection of any carcinogenic HPV genotype. When the HPV results were categorized hierarchically according to cervical cancer risk, there was no difference in the distribution of the HPV results for the clinician- and self-collected specimens (p= 0.7). This study concludes that FTA elute cartridge is a promising method of specimen transport for cervical cancer screening programs considering using self-collected specimen and HPV testing. Larger studies with clinical endpoints are now needed to assess the clinical performance.

Original languageEnglish (US)
Pages (from-to)167-171
Number of pages5
JournalJournal of Virological Methods
Volume189
Issue number1
DOIs
StatePublished - Apr 2013
Externally publishedYes

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Uterine Cervical Neoplasms
Early Detection of Cancer
Genotype
Equipment and Supplies
DNA
Clinical Studies

Keywords

  • Cervical cancer
  • FTA cartridge
  • Human papillomavirus (HPV)
  • Self-collection

ASJC Scopus subject areas

  • Virology

Cite this

Agreement for HPV genotyping detection between self-collected specimens on a FTA cartridge and clinician-collected specimens. / Guan, YaoYao; Gravitt, Patti E.; Howard, Roslyn; Eby, Yolanda J.; Wang, Shaoming; Li, Belinda; Feng, Changyan; Qiao, You Lin; Castle, Philip E.

In: Journal of Virological Methods, Vol. 189, No. 1, 04.2013, p. 167-171.

Research output: Contribution to journalArticle

Guan, YaoYao ; Gravitt, Patti E. ; Howard, Roslyn ; Eby, Yolanda J. ; Wang, Shaoming ; Li, Belinda ; Feng, Changyan ; Qiao, You Lin ; Castle, Philip E. / Agreement for HPV genotyping detection between self-collected specimens on a FTA cartridge and clinician-collected specimens. In: Journal of Virological Methods. 2013 ; Vol. 189, No. 1. pp. 167-171.
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