Aging-related anatomical and biochemical changes in lymphatic collectors impair lymph transport, fluid homeostasis, and pathogen clearance

Valerio Zolla, Irina Tsoy Nizamutdinova, Brian Scharf, Cristina C. Clement, Daisuke Maejima, Tony Akl, Takashi Nagai, Paola Luciani, Jean Christophe Leroux, Cornelia Halin, Sabriya Stukes, Sangeeta Tiwari, Arturo Casadevall, William R. Jacobs, David R. Entenberg, David C. Zawieja, John S. Condeelis, David R. Fooksman, Anatoliy A. Gashev, Laura Santambrogio

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Summary: The role of lymphatic vessels is to transport fluid, soluble molecules, and immune cells to the draining lymph nodes. Here, we analyze how the aging process affects the functionality of the lymphatic collectors and the dynamics of lymph flow. Ultrastructural, biochemical, and proteomic analysis indicates a loss of matrix proteins, and smooth muscle cells in aged collectors resulting in a decrease in contraction frequency, systolic lymph flow velocity, and pumping activity, as measured in vivo in lymphatic collectors. Functionally, this impairment also translated into a reduced ability for in vivo bacterial transport as determined by time-lapse microscopy. Ultrastructural and proteomic analysis also indicates a decrease in the thickness of the endothelial cell glycocalyx and loss of gap junction proteins in aged lymph collectors. Redox proteomic analysis mapped an aging-related increase in the glycation and carboxylation of lymphatic's endothelial cell and matrix proteins. Functionally, these modifications translate into apparent hyperpermeability of the lymphatics with pathogen escaping from the collectors into the surrounding tissue and a decreased ability to control tissue fluid homeostasis. Altogether, our data provide a mechanistic analysis of how the anatomical and biochemical changes, occurring in aged lymphatic vessels, compromise lymph flow, tissue fluid homeostasis, and pathogen transport.

Original languageEnglish (US)
JournalAging Cell
DOIs
StateAccepted/In press - 2015

Fingerprint

Lymph
Homeostasis
Proteomics
Lymphatic Vessels
Endothelial Cells
Glycocalyx
Connexins
Oxidation-Reduction
Smooth Muscle Myocytes
Microscopy
Proteins
Lymph Nodes

Keywords

  • Aging
  • Lymphatics
  • Mass spectrometry
  • Oxidative stress
  • Proteomics

ASJC Scopus subject areas

  • Cell Biology
  • Aging

Cite this

Aging-related anatomical and biochemical changes in lymphatic collectors impair lymph transport, fluid homeostasis, and pathogen clearance. / Zolla, Valerio; Nizamutdinova, Irina Tsoy; Scharf, Brian; Clement, Cristina C.; Maejima, Daisuke; Akl, Tony; Nagai, Takashi; Luciani, Paola; Leroux, Jean Christophe; Halin, Cornelia; Stukes, Sabriya; Tiwari, Sangeeta; Casadevall, Arturo; Jacobs, William R.; Entenberg, David R.; Zawieja, David C.; Condeelis, John S.; Fooksman, David R.; Gashev, Anatoliy A.; Santambrogio, Laura.

In: Aging Cell, 2015.

Research output: Contribution to journalArticle

Zolla, V, Nizamutdinova, IT, Scharf, B, Clement, CC, Maejima, D, Akl, T, Nagai, T, Luciani, P, Leroux, JC, Halin, C, Stukes, S, Tiwari, S, Casadevall, A, Jacobs, WR, Entenberg, DR, Zawieja, DC, Condeelis, JS, Fooksman, DR, Gashev, AA & Santambrogio, L 2015, 'Aging-related anatomical and biochemical changes in lymphatic collectors impair lymph transport, fluid homeostasis, and pathogen clearance', Aging Cell. https://doi.org/10.1111/acel.12330
Zolla, Valerio ; Nizamutdinova, Irina Tsoy ; Scharf, Brian ; Clement, Cristina C. ; Maejima, Daisuke ; Akl, Tony ; Nagai, Takashi ; Luciani, Paola ; Leroux, Jean Christophe ; Halin, Cornelia ; Stukes, Sabriya ; Tiwari, Sangeeta ; Casadevall, Arturo ; Jacobs, William R. ; Entenberg, David R. ; Zawieja, David C. ; Condeelis, John S. ; Fooksman, David R. ; Gashev, Anatoliy A. ; Santambrogio, Laura. / Aging-related anatomical and biochemical changes in lymphatic collectors impair lymph transport, fluid homeostasis, and pathogen clearance. In: Aging Cell. 2015.
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AU - Nizamutdinova, Irina Tsoy

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AU - Clement, Cristina C.

AU - Maejima, Daisuke

AU - Akl, Tony

AU - Nagai, Takashi

AU - Luciani, Paola

AU - Leroux, Jean Christophe

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AU - Stukes, Sabriya

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AU - Gashev, Anatoliy A.

AU - Santambrogio, Laura

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N2 - Summary: The role of lymphatic vessels is to transport fluid, soluble molecules, and immune cells to the draining lymph nodes. Here, we analyze how the aging process affects the functionality of the lymphatic collectors and the dynamics of lymph flow. Ultrastructural, biochemical, and proteomic analysis indicates a loss of matrix proteins, and smooth muscle cells in aged collectors resulting in a decrease in contraction frequency, systolic lymph flow velocity, and pumping activity, as measured in vivo in lymphatic collectors. Functionally, this impairment also translated into a reduced ability for in vivo bacterial transport as determined by time-lapse microscopy. Ultrastructural and proteomic analysis also indicates a decrease in the thickness of the endothelial cell glycocalyx and loss of gap junction proteins in aged lymph collectors. Redox proteomic analysis mapped an aging-related increase in the glycation and carboxylation of lymphatic's endothelial cell and matrix proteins. Functionally, these modifications translate into apparent hyperpermeability of the lymphatics with pathogen escaping from the collectors into the surrounding tissue and a decreased ability to control tissue fluid homeostasis. Altogether, our data provide a mechanistic analysis of how the anatomical and biochemical changes, occurring in aged lymphatic vessels, compromise lymph flow, tissue fluid homeostasis, and pathogen transport.

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