Aging per se is an independent risk factor for cholesterol gallstone formation in gallstone susceptible mice

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Cholesterol gallstones occur rarely in childhood and adolescence and increase linearly with age in both genders. To explore whether aging per se increases cholesterol saturation of bile and gallstone prevalence, and to investigate age-related changes in hepatic and biliary lipid metabolism, we studied gallstone-susceptible C57L mice and resistant AKR mice of both genders fed 8 weeks with a lithogenic diet containing 1% cholesterol, 0.5% cholic acid, and 15% butter fat starting at (young adult) 8, (older adult) 36, and (aged) 50-weeks-of-age. After the 8-week feeding, gallstone prevalence, gallbladder size, biliary lipid secretion rate, and HMG-CoA reductase activity were significantly greater but cholesterol 7α-hydroxylase activity was lower in C57L mice of both genders compared with AKR mice. Increasing age augmented biliary secretion and intestinal absorption of cholesterol, reduced hepatic synthesis and biliary secretion of bile salts, and decreased gallbladder contractility, all of which increased susceptibility to cholesterol cholelithiasis in C57L mice. We conclude that aging per se is an independent risk factor for cholesterol gallstone formation. Because aging increases significantly biliary cholesterol hypersecretion and gallstone prevalence in C57L mice carrying Lith genes, it is highly like that Longevity (aging) genes can enhance lithogenesis of Lith (gallstone) genes.

Original languageEnglish (US)
Pages (from-to)1950-1959
Number of pages10
JournalJournal of Lipid Research
Volume43
Issue number11
DOIs
StatePublished - Nov 1 2002
Externally publishedYes

Fingerprint

Gallstones
Aging of materials
Cholesterol
Inbred AKR Mouse
Genes
Gallbladder
Cholesterol 7-alpha-Hydroxylase
Hydroxymethylglutaryl CoA Reductases
Cholic Acid
Butter
Cholelithiasis
Liver
Intestinal Absorption
Nutrition
Bile Acids and Salts
Lipid Metabolism
Bile
Young Adult
Fats
Diet

Keywords

  • Bile flow
  • Bile salt
  • Cholesterol 7α-hydroxylase
  • Gallbladder size
  • HMG-CoA reductase
  • Mucin gel
  • Phospholipid

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Aging per se is an independent risk factor for cholesterol gallstone formation in gallstone susceptible mice. / Wang, David Q.H.

In: Journal of Lipid Research, Vol. 43, No. 11, 01.11.2002, p. 1950-1959.

Research output: Contribution to journalArticle

@article{4eeafc42a89c474f8f9bfd1eac47fd37,
title = "Aging per se is an independent risk factor for cholesterol gallstone formation in gallstone susceptible mice",
abstract = "Cholesterol gallstones occur rarely in childhood and adolescence and increase linearly with age in both genders. To explore whether aging per se increases cholesterol saturation of bile and gallstone prevalence, and to investigate age-related changes in hepatic and biliary lipid metabolism, we studied gallstone-susceptible C57L mice and resistant AKR mice of both genders fed 8 weeks with a lithogenic diet containing 1{\%} cholesterol, 0.5{\%} cholic acid, and 15{\%} butter fat starting at (young adult) 8, (older adult) 36, and (aged) 50-weeks-of-age. After the 8-week feeding, gallstone prevalence, gallbladder size, biliary lipid secretion rate, and HMG-CoA reductase activity were significantly greater but cholesterol 7α-hydroxylase activity was lower in C57L mice of both genders compared with AKR mice. Increasing age augmented biliary secretion and intestinal absorption of cholesterol, reduced hepatic synthesis and biliary secretion of bile salts, and decreased gallbladder contractility, all of which increased susceptibility to cholesterol cholelithiasis in C57L mice. We conclude that aging per se is an independent risk factor for cholesterol gallstone formation. Because aging increases significantly biliary cholesterol hypersecretion and gallstone prevalence in C57L mice carrying Lith genes, it is highly like that Longevity (aging) genes can enhance lithogenesis of Lith (gallstone) genes.",
keywords = "Bile flow, Bile salt, Cholesterol 7α-hydroxylase, Gallbladder size, HMG-CoA reductase, Mucin gel, Phospholipid",
author = "Wang, {David Q.H.}",
year = "2002",
month = "11",
day = "1",
doi = "10.1194/jlr.M200078-JLR200",
language = "English (US)",
volume = "43",
pages = "1950--1959",
journal = "Journal of Lipid Research",
issn = "0022-2275",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "11",

}

TY - JOUR

T1 - Aging per se is an independent risk factor for cholesterol gallstone formation in gallstone susceptible mice

AU - Wang, David Q.H.

PY - 2002/11/1

Y1 - 2002/11/1

N2 - Cholesterol gallstones occur rarely in childhood and adolescence and increase linearly with age in both genders. To explore whether aging per se increases cholesterol saturation of bile and gallstone prevalence, and to investigate age-related changes in hepatic and biliary lipid metabolism, we studied gallstone-susceptible C57L mice and resistant AKR mice of both genders fed 8 weeks with a lithogenic diet containing 1% cholesterol, 0.5% cholic acid, and 15% butter fat starting at (young adult) 8, (older adult) 36, and (aged) 50-weeks-of-age. After the 8-week feeding, gallstone prevalence, gallbladder size, biliary lipid secretion rate, and HMG-CoA reductase activity were significantly greater but cholesterol 7α-hydroxylase activity was lower in C57L mice of both genders compared with AKR mice. Increasing age augmented biliary secretion and intestinal absorption of cholesterol, reduced hepatic synthesis and biliary secretion of bile salts, and decreased gallbladder contractility, all of which increased susceptibility to cholesterol cholelithiasis in C57L mice. We conclude that aging per se is an independent risk factor for cholesterol gallstone formation. Because aging increases significantly biliary cholesterol hypersecretion and gallstone prevalence in C57L mice carrying Lith genes, it is highly like that Longevity (aging) genes can enhance lithogenesis of Lith (gallstone) genes.

AB - Cholesterol gallstones occur rarely in childhood and adolescence and increase linearly with age in both genders. To explore whether aging per se increases cholesterol saturation of bile and gallstone prevalence, and to investigate age-related changes in hepatic and biliary lipid metabolism, we studied gallstone-susceptible C57L mice and resistant AKR mice of both genders fed 8 weeks with a lithogenic diet containing 1% cholesterol, 0.5% cholic acid, and 15% butter fat starting at (young adult) 8, (older adult) 36, and (aged) 50-weeks-of-age. After the 8-week feeding, gallstone prevalence, gallbladder size, biliary lipid secretion rate, and HMG-CoA reductase activity were significantly greater but cholesterol 7α-hydroxylase activity was lower in C57L mice of both genders compared with AKR mice. Increasing age augmented biliary secretion and intestinal absorption of cholesterol, reduced hepatic synthesis and biliary secretion of bile salts, and decreased gallbladder contractility, all of which increased susceptibility to cholesterol cholelithiasis in C57L mice. We conclude that aging per se is an independent risk factor for cholesterol gallstone formation. Because aging increases significantly biliary cholesterol hypersecretion and gallstone prevalence in C57L mice carrying Lith genes, it is highly like that Longevity (aging) genes can enhance lithogenesis of Lith (gallstone) genes.

KW - Bile flow

KW - Bile salt

KW - Cholesterol 7α-hydroxylase

KW - Gallbladder size

KW - HMG-CoA reductase

KW - Mucin gel

KW - Phospholipid

UR - http://www.scopus.com/inward/record.url?scp=0036846873&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036846873&partnerID=8YFLogxK

U2 - 10.1194/jlr.M200078-JLR200

DO - 10.1194/jlr.M200078-JLR200

M3 - Article

C2 - 12401894

AN - SCOPUS:0036846873

VL - 43

SP - 1950

EP - 1959

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 11

ER -