Aggravation of inflammatory response by costimulation with titanium particles and mechanical perturbations in osteoblast- and macrophage-like cells

Heon Goo Lee, Anny Hsu, Hana Goto, Saqib Nizami, Jonathan H. Lee, Edwin R. Cadet, Peter Tang, Roya Shaji, Chandhanarat Chandhanayinyong, Seok Hyun Kweon, Daniel S. Oh, Hesham Tawfeek, Francis Y. Lee

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The interface between bone tissue and metal implants undergoes various types of mechanical loading, such as strain, compression, fluid pressure, and shear stress, from daily activities. Such mechanical perturbations create suboptimal environments at the host bone-implant junction, causing an accumulation of wear particles and debilitating osseous integration, potentially leading to implant failure. While many studies have focused on the effect of particles on macrophages or osteoprogenitor cells, differential and combined effects of mechanical perturbations and particles on such cell types have not been extensively studied. In this study, macrophages and osteoprogenitor cells were subjected to physiological and superphysiological mechanical stimuli in the presence and absence of Ti particles with the aim of simulating various microenvironments of the host bone-implant junction. Macrophages and osteoprogenitor cells were capable of engulfing Ti particles through actin remodeling and also exhibited changes in mRNA levels of proinflammatory cytokines under certain conditions. In osteoprogenitor cells, superphysiological strain increased proinflammatory gene expression; in macrophages, such mechanical perturbations did not affect gene expression. We confirmed that this phenomenon in osteoprogenitor cells occurred via activation of the ERK1/2 signaling pathway as a result of damage to the cytoplasmic membrane. Furthermore, AZD6244, a clinically relevant inhibitor of the ERK1/2 pathway, mitigated particle-induced inflammatory gene expression in osteoprogenitor cells and macrophages. This study provides evidence of more inflammatory responses under mechanical strains in osteoprogenitor cells than macrophages. Phagocytosis of particles and mechanical perturbation costimulate the ERK1/2 pathway, leading to expression of proinflammatory genes.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume304
Issue number5
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

Titanium
Osteoblasts
Macrophages
MAP Kinase Signaling System
Gene Expression
Bone and Bones
Phagocytosis
Actins
Metals
Cell Membrane
Cytokines
Pressure
Messenger RNA

Keywords

  • Actin
  • AZD6244
  • ERK
  • Osteolysis
  • Particle
  • Strain

ASJC Scopus subject areas

  • Cell Biology
  • Physiology

Cite this

Aggravation of inflammatory response by costimulation with titanium particles and mechanical perturbations in osteoblast- and macrophage-like cells. / Lee, Heon Goo; Hsu, Anny; Goto, Hana; Nizami, Saqib; Lee, Jonathan H.; Cadet, Edwin R.; Tang, Peter; Shaji, Roya; Chandhanayinyong, Chandhanarat; Kweon, Seok Hyun; Oh, Daniel S.; Tawfeek, Hesham; Lee, Francis Y.

In: American Journal of Physiology - Cell Physiology, Vol. 304, No. 5, 2013.

Research output: Contribution to journalArticle

Lee, Heon Goo ; Hsu, Anny ; Goto, Hana ; Nizami, Saqib ; Lee, Jonathan H. ; Cadet, Edwin R. ; Tang, Peter ; Shaji, Roya ; Chandhanayinyong, Chandhanarat ; Kweon, Seok Hyun ; Oh, Daniel S. ; Tawfeek, Hesham ; Lee, Francis Y. / Aggravation of inflammatory response by costimulation with titanium particles and mechanical perturbations in osteoblast- and macrophage-like cells. In: American Journal of Physiology - Cell Physiology. 2013 ; Vol. 304, No. 5.
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AU - Cadet, Edwin R.

AU - Tang, Peter

AU - Shaji, Roya

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AB - The interface between bone tissue and metal implants undergoes various types of mechanical loading, such as strain, compression, fluid pressure, and shear stress, from daily activities. Such mechanical perturbations create suboptimal environments at the host bone-implant junction, causing an accumulation of wear particles and debilitating osseous integration, potentially leading to implant failure. While many studies have focused on the effect of particles on macrophages or osteoprogenitor cells, differential and combined effects of mechanical perturbations and particles on such cell types have not been extensively studied. In this study, macrophages and osteoprogenitor cells were subjected to physiological and superphysiological mechanical stimuli in the presence and absence of Ti particles with the aim of simulating various microenvironments of the host bone-implant junction. Macrophages and osteoprogenitor cells were capable of engulfing Ti particles through actin remodeling and also exhibited changes in mRNA levels of proinflammatory cytokines under certain conditions. In osteoprogenitor cells, superphysiological strain increased proinflammatory gene expression; in macrophages, such mechanical perturbations did not affect gene expression. We confirmed that this phenomenon in osteoprogenitor cells occurred via activation of the ERK1/2 signaling pathway as a result of damage to the cytoplasmic membrane. Furthermore, AZD6244, a clinically relevant inhibitor of the ERK1/2 pathway, mitigated particle-induced inflammatory gene expression in osteoprogenitor cells and macrophages. This study provides evidence of more inflammatory responses under mechanical strains in osteoprogenitor cells than macrophages. Phagocytosis of particles and mechanical perturbation costimulate the ERK1/2 pathway, leading to expression of proinflammatory genes.

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