Age-related induction and disappearance of carcinogen-DNA-adducts in livers of rats exposed to low levels of 2-acetylaminofluorene

E. Mullaart, M. E T I Boerrigter, P. H M Lohman, Jan Vijg

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

It was investigated whether in vivo aging of rat liver is associated with changes in the induction and rate of disappearance of DNA damage. For this purpose 6- and 36-month-old rats were intraperitoneally injected with a single, low dose (5 mg/kg body wt.) of the model liver carcinogen 2-acetylaminofluorene (AAF). Using the 32P-postlabeling assay we found that N-(deoxyguanosin-8-yl)-2-aminofluorene (dG-C8-AF) was the major DNA-adduct formed. The minor adduct N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-C8-AAF) could only be detected after doses of 20 mg/kg or more. Quantitation of adduct levels at various time points after treatment indicated a rapid induction of AF-adducts, which were already present at 6 h after treatment. The subsequent loss of AF-adducts was relatively slow, as was indicated by the presence of a substantial amount of AF-adducts as late as 21 days after treatment. Slight age-related differences in the pattern of induction and disappearance of AF-adducts and a somewhat higher level of persisting lesions in old than in young rats were observed.

Original languageEnglish (US)
Pages (from-to)373-384
Number of pages12
JournalChemico-Biological Interactions
Volume69
Issue number4
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

2-Acetylaminofluorene
DNA Adducts
Carcinogens
Liver
Rats
DNA Damage
Assays
Aging of materials
DNA
N-(deoxyguanosin-8-yl)-2-aminofluorene
N-(deoxyguanosin-8-yl)acetylaminofluorene

Keywords

  • 2-Acetylaminofluorene
  • Aging
  • DNA repair
  • Persisting adducts
  • Rat liver

ASJC Scopus subject areas

  • Toxicology

Cite this

Age-related induction and disappearance of carcinogen-DNA-adducts in livers of rats exposed to low levels of 2-acetylaminofluorene. / Mullaart, E.; Boerrigter, M. E T I; Lohman, P. H M; Vijg, Jan.

In: Chemico-Biological Interactions, Vol. 69, No. 4, 1989, p. 373-384.

Research output: Contribution to journalArticle

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