Age-dependent accumulation of alkali-labile sites in DNA of post-mitotic but not in that of mitotic rat liver cells

E. Mullaart, M. E.T.I. Boerrigter, A. Brouwer, F. Berends, J. Vijg

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

The amount of spontaneous damage in the DNA of rat liver cells was measured by using the alkaline elution assay. An age-related increase of approximately 700 detectable alkali-labile sites (80%) was found for rat parenchymal liver cells; cells from 6-month-old rats contained approximately 900 alkali-labile sites per cell while cells from 36-month-old rats contained approximately 1600 alkali-labile sites. In contrast to the situation with the postmitotic parenchymal liver cells, no age-related increase in the number of alkali-labile sites was found for the non-parenchymal liver cell fraction, which has a higher mitotic activity. These results support the hypothesis that aging takes place predominantly in postmitotic cells.

Original languageEnglish (US)
Pages (from-to)41-49
Number of pages9
JournalMechanisms of Ageing and Development
Volume45
Issue number1
DOIs
StatePublished - Oct 1988
Externally publishedYes

Keywords

  • Accumulation of DNA damage
  • Aging
  • Alkaline elution
  • Rat liver cells

ASJC Scopus subject areas

  • Aging
  • Developmental Biology

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