Age-dependent accumulation of alkali-labile sites in DNA of post-mitotic but not in that of mitotic rat liver cells

E. Mullaart, M. E T I Boerrigter, A. Brouwer, F. Berends, Jan Vijg

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The amount of spontaneous damage in the DNA of rat liver cells was measured by using the alkaline elution assay. An age-related increase of approximately 700 detectable alkali-labile sites (80%) was found for rat parenchymal liver cells; cells from 6-month-old rats contained approximately 900 alkali-labile sites per cell while cells from 36-month-old rats contained approximately 1600 alkali-labile sites. In contrast to the situation with the postmitotic parenchymal liver cells, no age-related increase in the number of alkali-labile sites was found for the non-parenchymal liver cell fraction, which has a higher mitotic activity. These results support the hypothesis that aging takes place predominantly in postmitotic cells.

Original languageEnglish (US)
Pages (from-to)41-49
Number of pages9
JournalMechanisms of Ageing and Development
Volume45
Issue number1
DOIs
StatePublished - 1988
Externally publishedYes

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Alkalies
Liver
Rats
DNA
Assays
Aging of materials
DNA Damage

Keywords

  • Accumulation of DNA damage
  • Aging
  • Alkaline elution
  • Rat liver cells

ASJC Scopus subject areas

  • Aging
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

Age-dependent accumulation of alkali-labile sites in DNA of post-mitotic but not in that of mitotic rat liver cells. / Mullaart, E.; Boerrigter, M. E T I; Brouwer, A.; Berends, F.; Vijg, Jan.

In: Mechanisms of Ageing and Development, Vol. 45, No. 1, 1988, p. 41-49.

Research output: Contribution to journalArticle

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AU - Vijg, Jan

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Y1 - 1988

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