Advantages of tandem LC-MS for the rapid assessment of tissue-specific metabolic complexity using a pentafluorophenylpropyl stationary phase

Haitao Lv, Gustavo Palacios, Kirsten Hartil, Irwin J. Kurland

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

In this study, a tandem LC-MS (Waters Xevo TQ) MRM-based MS method was developed for rapid, broad profiling of hydrophilic metabolites from biological samples, in either positive or negative ion modes without the need for an ion pairing reagent, using a reversed-phase pentafluorophenylpropyl (PFPP) column. The developed method was successfully applied to analyze various biological samples from C57BL/6 mice, including urine, duodenum, liver, plasma, kidney, heart, and skeletal muscle. As result, a total 112 of hydrophilic metabolites were detected within 8 min of running time to obtain a metabolite profile of the biological samples. The analysis of this number of hydrophilic metabolites is significantly faster than previous studies. Classification separation for metabolites from different tissues was globally analyzed by PCA, PLS-DA and HCA biostatistical methods. Overall, most of the hydrophilic metabolites were found to have a "fingerprint" characteristic of tissue dependency. In general, a higher level of most metabolites was found in urine, duodenum, and kidney. Altogether, these results suggest that this method has potential application for targeted metabolomic analyzes of hydrophilic metabolites in a wide ranges of biological samples.

Original languageEnglish (US)
Pages (from-to)2104-2112
Number of pages9
JournalJournal of Proteome Research
Volume10
Issue number4
DOIs
StatePublished - Apr 1 2011

Keywords

  • C57BL/6 mice
  • LC-MS
  • MRM
  • hydrophilic metabolites
  • metabolic complexity
  • metabolic networks
  • metabolomics
  • reversed-phase HPLC

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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