Advances in time-resolved approaches to characterize the dynamical nature of enzymatic catalysis

Time-resolved studies of enzymes date more than 100 years ago with the success of the Michaelis-Menton description of enzyme kinetics. For more than a century, approach development has been crucial toward the goal of a determination of the time evolution of atomic structure. To date, proven techniques to study enzymes include laser-induced temperature-jump (T-jump), time-resolved spectroscopic probes, laser-induced pH-Jump and rapid mixing methods. One way or another, these techniques have been applied to the study of triosephosphate isomerase (TIM), lactate dehydrogenase (LDH)-laser-induced T-Jump studies, hydride transfer in transhydrogenase and hydrolysis reaction of the Ras protein.