Advances in engineering of fluorescent proteins and photoactivatable proteins with red emission

Kiryl D. Piatkevich, Vladislav Verkhusha

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Monomeric fluorescent proteins of different colors are widely used to study behavior and targeting of proteins in living cells. Fluorescent proteins that irreversibly change their spectral properties in response to light irradiation of a specific wavelength, or photoactivate, have become increasingly popular to image intracellular dynamics and superresolution protein localization. Until recently, however, no optimized monomeric red fluorescent proteins and red photoactivatable proteins have been available. Furthermore, monomeric fluorescent proteins, which change emission from blue to red simply with time, so-called fluorescent timers, were developed to study protein age and turnover. Understanding of chemical mechanisms of the chromophore maturation or photoactivation into a red form will further advance engineering of fluorescent timers and photoactivatable proteins with enhanced and novel properties.

Original languageEnglish (US)
Pages (from-to)23-29
Number of pages7
JournalCurrent Opinion in Chemical Biology
Volume14
Issue number1
DOIs
StatePublished - Feb 2010

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Protein Engineering
Proteins
Protein Transport
Chromophores
Color
Light
Cells
Irradiation
Wavelength

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry

Cite this

Advances in engineering of fluorescent proteins and photoactivatable proteins with red emission. / Piatkevich, Kiryl D.; Verkhusha, Vladislav.

In: Current Opinion in Chemical Biology, Vol. 14, No. 1, 02.2010, p. 23-29.

Research output: Contribution to journalArticle

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