ADP-induced rocking of the kinesin motor domain revealed by single-molecule fluorescence polarization microscopy

Hernando J. Sosa, Erwin J G Peterman, W. E. Moerner, Lawrence S B Goldstein

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Kinesin is an ATP-driven molecular motor protein that moves processively along microtubules. Despite considerable research, the detailed mechanism of kinesin motion remains elusive. We applied an enhanced suite of single- and multiple-molecule fluorescence polarization microscopy assays to report the orientation and mobility of kinesin molecules bound to microtubules as a function of nucleotide state. In the presence of analogs of ATP, ADP-Pi or in the absence of nucleotide, the kinesin head maintains a rigid orientation. In the presence of ADP, the motor domain of kinesin, still bound to the microtubule, adopts a previously undescribed, highly mobile state. This state may be general to the chemomechanical cycle of motor proteins; in the case of kinesin, the transition from a highly mobile to a rigid state after ADP release may contribute to the generation of the 8 nm step.

Original languageEnglish (US)
Pages (from-to)540-544
Number of pages5
JournalNature Structural Biology
Volume8
Issue number6
DOIs
StatePublished - 2001

Fingerprint

Polarization Microscopy
Kinesin
Fluorescence Polarization
Fluorescence Microscopy
Adenosine Diphosphate
Microscopic examination
Fluorescence
Polarization
Molecules
Microtubules
Nucleotides
Molecular Motor Proteins
Adenosine Triphosphate
Single Molecule Imaging
Assays
Research

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Genetics

Cite this

ADP-induced rocking of the kinesin motor domain revealed by single-molecule fluorescence polarization microscopy. / Sosa, Hernando J.; Peterman, Erwin J G; Moerner, W. E.; Goldstein, Lawrence S B.

In: Nature Structural Biology, Vol. 8, No. 6, 2001, p. 540-544.

Research output: Contribution to journalArticle

Sosa, Hernando J. ; Peterman, Erwin J G ; Moerner, W. E. ; Goldstein, Lawrence S B. / ADP-induced rocking of the kinesin motor domain revealed by single-molecule fluorescence polarization microscopy. In: Nature Structural Biology. 2001 ; Vol. 8, No. 6. pp. 540-544.
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