Adipocytes in both brown and white adipose tissue of adult mice are functionally connected via gap junctions

Implications for Chagas disease

Shoshana Burke, Fnu Nagajyothi, Mia M. Thi, Menachem Hanani, Philipp E. Scherer, Herbert B. Tanowitz, David C. Spray

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Adipose tissue serves as a host reservoir for the protozoan Trypanosoma cruzi, the causative organism in Chagas disease. Gap junctions interconnect cells of most tissues, serving to synchronize cell activities including secretion in glandular tissue, and we have previously demonstrated that gap junctions are altered in various tissues and cells infected with T. cruzi. Herein, we examined the gap junction protein connexin 43 (Cx43) expression in infected adipose tissues. Adipose tissue is the largest endocrine organ of the body and is also involved in other physiological functions. In mammals, it is primarily composed of white adipocytes. Although gap junctions are a prominent feature of brown adipocytes, they have not been explored extensively in white adipocytes, especially in the setting of infection. Thus, we examined functional coupling in both white and brown adipocytes in mice. Injection of electrical current or the dye Lucifer Yellow into adipocytes within fat tissue spread to adjacent cells, which was reduced by treatment with agents known to block gap junctions. Moreover, Cx43 was detected in both brown and white fat tissue. At thirty and ninety days post-infection, Cx43 was downregulated in brown adipocytes and upregulated in white adipocytes. Gap junction-mediated intercellular communication likely contributes to hormone secretion and other functions in white adipose tissue and to nonshivering thermogenesis in brown fat, and modulation of the coupling by T. cruzi infection is expected to impact these functions.

Original languageEnglish (US)
Pages (from-to)893-901
Number of pages9
JournalMicrobes and Infection
Volume16
Issue number11
DOIs
StatePublished - Nov 1 2014

Fingerprint

White Adipose Tissue
White Adipocytes
Brown Adipose Tissue
Chagas Disease
Gap Junctions
Adipocytes
Brown Adipocytes
Connexin 43
Trypanosoma cruzi
Adipose Tissue
Infection
Connexins
Thermogenesis
Mammals
Coloring Agents
Down-Regulation
Fats
Hormones
Injections

Keywords

  • Adipose tissue
  • Chagas disease
  • Connexin43
  • Gap junctions
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Adipocytes in both brown and white adipose tissue of adult mice are functionally connected via gap junctions : Implications for Chagas disease. / Burke, Shoshana; Nagajyothi, Fnu; Thi, Mia M.; Hanani, Menachem; Scherer, Philipp E.; Tanowitz, Herbert B.; Spray, David C.

In: Microbes and Infection, Vol. 16, No. 11, 01.11.2014, p. 893-901.

Research output: Contribution to journalArticle

Burke, Shoshana ; Nagajyothi, Fnu ; Thi, Mia M. ; Hanani, Menachem ; Scherer, Philipp E. ; Tanowitz, Herbert B. ; Spray, David C. / Adipocytes in both brown and white adipose tissue of adult mice are functionally connected via gap junctions : Implications for Chagas disease. In: Microbes and Infection. 2014 ; Vol. 16, No. 11. pp. 893-901.
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