Adhesion molecules as prognostic factors in nasopharyngeal carcinoma

Yelizaveta Shnayder, M. Abraham Kuriakose, Herman Yee, Fang An Chen, Mark D. DeLacure, Xiaonan (Nan) Xue, Jaishree Jagirdar

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objective/Hypothesis: To identify the significance of molecular markers in determining the risk of recurrence and distant metastases in nasopharyngeal carcinoma. Study Design: In this retrospective case study, we evaluated archival nasopharyngeal carcinoma specimens for patterns of expression of E-cadherin, β-catenin, c-erb-B2, and Ki-67, which have been demonstrated to be important in other tumors. Methods: Fifty-four cases of nasopharyngeal carcinoma were identified, with a maximum follow-up of 13 years. The histopathological sections were stained using an automated immunohistochemical stainer (NexES, Ventana Medical Systems, Tucson, AZ) for E-cadherin (Zymed Laboratories [San Francisco, CA] and Transduction Laboratories [Lexington, KY] clones), β-catenin (Zymed), c-erb-B2 (Ventana Medical Systems), and Ki-67 (Novocastra, Burlingame, CA). The numbers of positively staining cells were scored as follows: 0%, 1% to 33%, 34% to 66%, or greater than 67%. Results: E-cadherin (Zymed) stained positively in only one case. The Transduction Laboratories clone demonstrated a spectrum of staining in all cases, from complete to disrupted to no identifiable membranous staining. The staining was consistently absent at the advancing tumor border, regardless of stage. The loss of β-catenin expression did not correlate with that of E-cadherin or with clinical outcomes. No staining was identified for c-erb-B2. Ki-67 staining was variable and did not correlate with clinical outcomes. Conclusions: Altered expression or loss of E-cadherin, or both, may result in loss of function, particularly at the infiltrating edge, with resultant loss of cell polarity, cell migration, and eventual metastasis. The interpretation of E-cadherin staining depends on antibody source. In contrast to recent studies, β-catenin expression is not altered and c-erb-B2 expression not identified, suggesting that these markers are not important in the prognosis of nasopnaryngeal carcinoma.

Original languageEnglish (US)
Pages (from-to)1842-1846
Number of pages5
JournalLaryngoscope
Volume111
Issue number10
StatePublished - 2001
Externally publishedYes

Fingerprint

Cadherins
Catenins
Staining and Labeling
Clone Cells
Neoplasm Metastasis
Cell Polarity
San Francisco
Nasopharyngeal carcinoma
Cell Movement
Neoplasms
Retrospective Studies
Carcinoma
Recurrence
Antibodies

Keywords

  • Adhesion molecules
  • Immunohistochemistry
  • Molecular markers
  • Nasopharyngeal carcinoma.
  • Prognosis

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Shnayder, Y., Kuriakose, M. A., Yee, H., Chen, F. A., DeLacure, M. D., Xue, X. N., & Jagirdar, J. (2001). Adhesion molecules as prognostic factors in nasopharyngeal carcinoma. Laryngoscope, 111(10), 1842-1846.

Adhesion molecules as prognostic factors in nasopharyngeal carcinoma. / Shnayder, Yelizaveta; Kuriakose, M. Abraham; Yee, Herman; Chen, Fang An; DeLacure, Mark D.; Xue, Xiaonan (Nan); Jagirdar, Jaishree.

In: Laryngoscope, Vol. 111, No. 10, 2001, p. 1842-1846.

Research output: Contribution to journalArticle

Shnayder, Y, Kuriakose, MA, Yee, H, Chen, FA, DeLacure, MD, Xue, XN & Jagirdar, J 2001, 'Adhesion molecules as prognostic factors in nasopharyngeal carcinoma', Laryngoscope, vol. 111, no. 10, pp. 1842-1846.
Shnayder Y, Kuriakose MA, Yee H, Chen FA, DeLacure MD, Xue XN et al. Adhesion molecules as prognostic factors in nasopharyngeal carcinoma. Laryngoscope. 2001;111(10):1842-1846.
Shnayder, Yelizaveta ; Kuriakose, M. Abraham ; Yee, Herman ; Chen, Fang An ; DeLacure, Mark D. ; Xue, Xiaonan (Nan) ; Jagirdar, Jaishree. / Adhesion molecules as prognostic factors in nasopharyngeal carcinoma. In: Laryngoscope. 2001 ; Vol. 111, No. 10. pp. 1842-1846.
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abstract = "Objective/Hypothesis: To identify the significance of molecular markers in determining the risk of recurrence and distant metastases in nasopharyngeal carcinoma. Study Design: In this retrospective case study, we evaluated archival nasopharyngeal carcinoma specimens for patterns of expression of E-cadherin, β-catenin, c-erb-B2, and Ki-67, which have been demonstrated to be important in other tumors. Methods: Fifty-four cases of nasopharyngeal carcinoma were identified, with a maximum follow-up of 13 years. The histopathological sections were stained using an automated immunohistochemical stainer (NexES, Ventana Medical Systems, Tucson, AZ) for E-cadherin (Zymed Laboratories [San Francisco, CA] and Transduction Laboratories [Lexington, KY] clones), β-catenin (Zymed), c-erb-B2 (Ventana Medical Systems), and Ki-67 (Novocastra, Burlingame, CA). The numbers of positively staining cells were scored as follows: 0{\%}, 1{\%} to 33{\%}, 34{\%} to 66{\%}, or greater than 67{\%}. Results: E-cadherin (Zymed) stained positively in only one case. The Transduction Laboratories clone demonstrated a spectrum of staining in all cases, from complete to disrupted to no identifiable membranous staining. The staining was consistently absent at the advancing tumor border, regardless of stage. The loss of β-catenin expression did not correlate with that of E-cadherin or with clinical outcomes. No staining was identified for c-erb-B2. Ki-67 staining was variable and did not correlate with clinical outcomes. Conclusions: Altered expression or loss of E-cadherin, or both, may result in loss of function, particularly at the infiltrating edge, with resultant loss of cell polarity, cell migration, and eventual metastasis. The interpretation of E-cadherin staining depends on antibody source. In contrast to recent studies, β-catenin expression is not altered and c-erb-B2 expression not identified, suggesting that these markers are not important in the prognosis of nasopnaryngeal carcinoma.",
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AU - Kuriakose, M. Abraham

AU - Yee, Herman

AU - Chen, Fang An

AU - DeLacure, Mark D.

AU - Xue, Xiaonan (Nan)

AU - Jagirdar, Jaishree

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N2 - Objective/Hypothesis: To identify the significance of molecular markers in determining the risk of recurrence and distant metastases in nasopharyngeal carcinoma. Study Design: In this retrospective case study, we evaluated archival nasopharyngeal carcinoma specimens for patterns of expression of E-cadherin, β-catenin, c-erb-B2, and Ki-67, which have been demonstrated to be important in other tumors. Methods: Fifty-four cases of nasopharyngeal carcinoma were identified, with a maximum follow-up of 13 years. The histopathological sections were stained using an automated immunohistochemical stainer (NexES, Ventana Medical Systems, Tucson, AZ) for E-cadherin (Zymed Laboratories [San Francisco, CA] and Transduction Laboratories [Lexington, KY] clones), β-catenin (Zymed), c-erb-B2 (Ventana Medical Systems), and Ki-67 (Novocastra, Burlingame, CA). The numbers of positively staining cells were scored as follows: 0%, 1% to 33%, 34% to 66%, or greater than 67%. Results: E-cadherin (Zymed) stained positively in only one case. The Transduction Laboratories clone demonstrated a spectrum of staining in all cases, from complete to disrupted to no identifiable membranous staining. The staining was consistently absent at the advancing tumor border, regardless of stage. The loss of β-catenin expression did not correlate with that of E-cadherin or with clinical outcomes. No staining was identified for c-erb-B2. Ki-67 staining was variable and did not correlate with clinical outcomes. Conclusions: Altered expression or loss of E-cadherin, or both, may result in loss of function, particularly at the infiltrating edge, with resultant loss of cell polarity, cell migration, and eventual metastasis. The interpretation of E-cadherin staining depends on antibody source. In contrast to recent studies, β-catenin expression is not altered and c-erb-B2 expression not identified, suggesting that these markers are not important in the prognosis of nasopnaryngeal carcinoma.

AB - Objective/Hypothesis: To identify the significance of molecular markers in determining the risk of recurrence and distant metastases in nasopharyngeal carcinoma. Study Design: In this retrospective case study, we evaluated archival nasopharyngeal carcinoma specimens for patterns of expression of E-cadherin, β-catenin, c-erb-B2, and Ki-67, which have been demonstrated to be important in other tumors. Methods: Fifty-four cases of nasopharyngeal carcinoma were identified, with a maximum follow-up of 13 years. The histopathological sections were stained using an automated immunohistochemical stainer (NexES, Ventana Medical Systems, Tucson, AZ) for E-cadherin (Zymed Laboratories [San Francisco, CA] and Transduction Laboratories [Lexington, KY] clones), β-catenin (Zymed), c-erb-B2 (Ventana Medical Systems), and Ki-67 (Novocastra, Burlingame, CA). The numbers of positively staining cells were scored as follows: 0%, 1% to 33%, 34% to 66%, or greater than 67%. Results: E-cadherin (Zymed) stained positively in only one case. The Transduction Laboratories clone demonstrated a spectrum of staining in all cases, from complete to disrupted to no identifiable membranous staining. The staining was consistently absent at the advancing tumor border, regardless of stage. The loss of β-catenin expression did not correlate with that of E-cadherin or with clinical outcomes. No staining was identified for c-erb-B2. Ki-67 staining was variable and did not correlate with clinical outcomes. Conclusions: Altered expression or loss of E-cadherin, or both, may result in loss of function, particularly at the infiltrating edge, with resultant loss of cell polarity, cell migration, and eventual metastasis. The interpretation of E-cadherin staining depends on antibody source. In contrast to recent studies, β-catenin expression is not altered and c-erb-B2 expression not identified, suggesting that these markers are not important in the prognosis of nasopnaryngeal carcinoma.

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