Adaptability of the semi-invariant natural killer T-cell receptor towards structurally diverse CD1d-restricted ligands

Sebastian Joyce, William C. Florence, Chengfeng Xia, Laura E. Gordy, Wenlan Chen, Yalong Zhang, James Scott-Browne, Yuki Kinjo, Karl O A Yu, Santosh Keshipeddy, Daniel G. Pellicci, Onisha Patel, Lars Kjer-Nielsen, James McCluskey, Dale I. Godfrey, Jamie Rossjohn, Stewart K. Richardson, Steven A. Porcelli, Amy R. Howell, Kyoko HayakawaLaurent Gapin, Dirk M. Zajonc, Peng George Wang

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The semi-invariant natural killer (NK) T-cell receptor (NKTcr) recognises structurally diverse glycolipid antigens presented by the monomorphic CD1d molecule. While the α-chain of the NKTcr is invariant, the Β-chain is more diverse, but how this diversity enables the NKTcr to recognise diverse antigens, such as an α-linked monosaccharide (α-galactosylceramide and α-galactosyldiacylglycerol) and the Β-linked trisaccharide (isoglobotriaosylceramide), is unclear. We demonstrate here that NKTcrs, which varied in their Β-chain usage, recognised diverse glycolipid antigens with a similar binding mode on CD1d. Nevertheless, the NKTcrs recognised distinct epitopic sites within these antigens, including α-galactosylceramide, the structurally similar α-galactosyldiacylglycerol and the very distinct isoglobotriaosylceramide. We also show that the relative roles of the CDR loops within the NKTcr Β-chain varied as a function of the antigen. Thus, while NKTcrs characteristically use a conserved docking mode, the NKTcr Β-chain allows these cells to recognise unique aspects of structurally diverse CD1d-restricted ligands.

Original languageEnglish (US)
Pages (from-to)3579-3590
Number of pages12
JournalEMBO Journal
Volume28
Issue number22
DOIs
StatePublished - Nov 2009

Fingerprint

Natural Killer Cell Receptors
Natural Killer T-Cells
T-Cell Antigen Receptor
Ligands
Antigens
Galactosylceramides
Glycolipids
CD1d Antigen
Trisaccharides
Monosaccharides

Keywords

  • Antigen recognition
  • Glycolipid antigens
  • NKT cells
  • Recognition logic
  • Semi-invariant T-cell receptor

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Joyce, S., Florence, W. C., Xia, C., Gordy, L. E., Chen, W., Zhang, Y., ... Wang, P. G. (2009). Adaptability of the semi-invariant natural killer T-cell receptor towards structurally diverse CD1d-restricted ligands. EMBO Journal, 28(22), 3579-3590. https://doi.org/10.1038/emboj.2009.286

Adaptability of the semi-invariant natural killer T-cell receptor towards structurally diverse CD1d-restricted ligands. / Joyce, Sebastian; Florence, William C.; Xia, Chengfeng; Gordy, Laura E.; Chen, Wenlan; Zhang, Yalong; Scott-Browne, James; Kinjo, Yuki; Yu, Karl O A; Keshipeddy, Santosh; Pellicci, Daniel G.; Patel, Onisha; Kjer-Nielsen, Lars; McCluskey, James; Godfrey, Dale I.; Rossjohn, Jamie; Richardson, Stewart K.; Porcelli, Steven A.; Howell, Amy R.; Hayakawa, Kyoko; Gapin, Laurent; Zajonc, Dirk M.; Wang, Peng George.

In: EMBO Journal, Vol. 28, No. 22, 11.2009, p. 3579-3590.

Research output: Contribution to journalArticle

Joyce, S, Florence, WC, Xia, C, Gordy, LE, Chen, W, Zhang, Y, Scott-Browne, J, Kinjo, Y, Yu, KOA, Keshipeddy, S, Pellicci, DG, Patel, O, Kjer-Nielsen, L, McCluskey, J, Godfrey, DI, Rossjohn, J, Richardson, SK, Porcelli, SA, Howell, AR, Hayakawa, K, Gapin, L, Zajonc, DM & Wang, PG 2009, 'Adaptability of the semi-invariant natural killer T-cell receptor towards structurally diverse CD1d-restricted ligands', EMBO Journal, vol. 28, no. 22, pp. 3579-3590. https://doi.org/10.1038/emboj.2009.286
Joyce, Sebastian ; Florence, William C. ; Xia, Chengfeng ; Gordy, Laura E. ; Chen, Wenlan ; Zhang, Yalong ; Scott-Browne, James ; Kinjo, Yuki ; Yu, Karl O A ; Keshipeddy, Santosh ; Pellicci, Daniel G. ; Patel, Onisha ; Kjer-Nielsen, Lars ; McCluskey, James ; Godfrey, Dale I. ; Rossjohn, Jamie ; Richardson, Stewart K. ; Porcelli, Steven A. ; Howell, Amy R. ; Hayakawa, Kyoko ; Gapin, Laurent ; Zajonc, Dirk M. ; Wang, Peng George. / Adaptability of the semi-invariant natural killer T-cell receptor towards structurally diverse CD1d-restricted ligands. In: EMBO Journal. 2009 ; Vol. 28, No. 22. pp. 3579-3590.
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