Acute but not chronic macrophage recruitment in filarial infections in mice is dependent on C-C chemokine ligand 2

Manish Ramesh, N. Paciorkowski, Y. Dash, L. Shultz, T. V. Rajan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Macrophages play an important role in the formation of granulomas and the clearance of Brugia pahangi infections in mice. However, the factors responsible for the recruitment of these cells to the site of infection are not known. In this study we examined the role of the C-C chemokine ligand 2 (CCL2; also known as macrophage chemotactic factor - MCP1) in macrophage recruitment in intraperitoneal infections with B. pahangi. We observed that CCL2 was expressed by peritoneal exudate cells and was present in the sera of wild-type mice. Serum levels of CCL2 peaked twice during the immune response, once during the early, acute phase and again during the late, chronic phase. To further elucidate the role of this chemokine in the anti-filarial immune response, we compared CCL2 deficient (CCL2-/-) mice to wild-type mice. We observed that macrophage recruitment was impaired only during the acute phase in the former. While macrophage recruitment was unaffected during the chronic phase, increased accumulation of B and T lymphocytes was seen in these mice. We further report that larval clearance and the in vitro adhesion of PECs to larvae were unimpaired in these mice.

Original languageEnglish (US)
Pages (from-to)395-404
Number of pages10
JournalParasite Immunology
Volume29
Issue number8
DOIs
StatePublished - Aug 2007
Externally publishedYes

Fingerprint

CC Chemokines
Macrophages
Ligands
Brugia pahangi
Infection
Chemotactic Factors
Exudates and Transudates
Granuloma
Serum
Chemokines
Larva
B-Lymphocytes
T-Lymphocytes

Keywords

  • Brugia
  • CCL2
  • Filaria
  • Inflammation
  • Macrophage
  • MCP1
  • Recruitment

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Cite this

Acute but not chronic macrophage recruitment in filarial infections in mice is dependent on C-C chemokine ligand 2. / Ramesh, Manish; Paciorkowski, N.; Dash, Y.; Shultz, L.; Rajan, T. V.

In: Parasite Immunology, Vol. 29, No. 8, 08.2007, p. 395-404.

Research output: Contribution to journalArticle

Ramesh, Manish ; Paciorkowski, N. ; Dash, Y. ; Shultz, L. ; Rajan, T. V. / Acute but not chronic macrophage recruitment in filarial infections in mice is dependent on C-C chemokine ligand 2. In: Parasite Immunology. 2007 ; Vol. 29, No. 8. pp. 395-404.
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